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Arifuzzaman M.,Khulna University | Khatun M.R.,National Institute of Biotechnology | Rahman H.,Khulna University
African Journal of Biotechnology | Year: 2010

A total of 55 actinomycetes isolates from soil sample of Karanjal region in Sundarbans were characterized for morphological identification and antimicrobial activity. Four general such as Actinomyces, Nocardia, Streptomyces and Micromonospora with total numbers of isolates were 27, 14, 11 and 3, respectively, were identified from the sample. Twenty actinomycetes isolates produced antibiotic against one or more gram-negative pathogenic bacteria such as Shigella boydii, Shigella flexneri-AN-31153, Shigella sonnei, Pseudomonas, Shigella dysenterriae type-1, Vibrio cholerae-0139, Salmonella typhi-Ao-12014, Plesiomonas, Hafnia spp., Vibrio cholerae-OGET, and Escherichia coli-186LT. The study indicated that Sundarbans' soil had diverse group of actinomycetes and three of the tested isolates had a broader spectrum antibacterial activity which showed potential as a source of antibiotics for pharmaceutical interest. © 2010 Academic Journals.

Abdus Subhan M.,Bangladesh University | Saifur Rahman M.,Bangladesh University | Alam K.,Bangladesh University | Mahmud Hasan M.,National Institute of Biotechnology
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy | Year: 2014

A novel ligand (E)-2-styryl-1H-imidazo [4, 5-f] [1, 10] phenanthroline(L) has been synthesized from 1,10-phenanthroline-5,6-dione. Its transition metal complexes, [FeLCl4][L-H] and [CuL2](NO3) 2 have also been synthesized. Besides, three mixed ligand lanthanide metal complexes of Phendione and β-diketones have been synthesized, namely [Eu(TFN)3(Phendione)] (TFN = 4,4,4-trifluoro-1(2-napthyl)-1,3- butanedione), [Eu(HFT)3(Phendione)] (HFT = 4,4,5,5,6,6,6-heptafluoro- 1-(2-thienyl)-1,3-hexanedione), [Yb(HFA)3(Phendione)] (hfa = hexafluoroacetylacetonate). The synthesized ligands and metal complexes have been characterized by FTIR, UV-Visible spectroscopy and PL spectra. DNA binding activities of the complexes and the ligands have been studied by DNA gel electrophoresis. DNA binding studies showed that Fe complex of the synthesized ligand is more potent DNA binding and damaging agent compare to others under study. The synthesized compounds were also screened for their antimicrobial activities by disc diffusion method against three microbes, namely Escherichia coli, Staphylococcus aureus, Proteus penneri. The lanthanide complexes of phendione showed great antibacterial activities. © 2013 Elsevier B.V. All rights reserved.

Khatun M.R.,National Institute of Biotechnology | Arifuzzaman M.,Khulna University | Ashraf A.,Khulna University
Asian Journal of Animal and Veterinary Advances | Year: 2011

Genetic abnormalities are not very common. About one third of total calves die due to various reasons, among these a significant number die from different disorder. When they do occur, they cause economic losses. One of the main constraints for cattle improvement is insufficient knowledge on chromosome inheritance pattern, different genetic disease and their chromosomal view. A slight slip in Chromosome number or structure brings obvious disorder though out the life, it may leads to death. Karyotype analysis is an effective way for identification of this disorder of chromosome. Identification of genetic disorder or unique character through karyotyping and possible remedy may protect the loss of cattle, which in turn increases the total productivity. Various abnormal karyotype, karyotype analysis, karyotype preparation were mainly reviewed in this study. © 2011 Academic Journals Inc.

Dash B.K.,Islamic University of Technology | Dash B.K.,Jessore University of Science and Technology | Rahman M.M.,Islamic University of Technology | Sarker P.K.,National Institute of Biotechnology
BioMed Research International | Year: 2015

A study was carried out with a newly isolated bacterial strain yielding extracellular amylase. The phylogenetic tree constructed on the basis of 16S rDNA gene sequences revealed this strain as clustered with the closest members of Bacillus sp. and identified as Bacillus subtilis BI19. The effect of various fermentation conditions on amylase production through shake-flask culture was investigated. Rice flour (1.25%) as a cheap natural carbon source was found to induce amylase production mostly. A combination of peptone and tryptone as organic and ammonium sulfate as inorganic nitrogen sources gave highest yield. Maximum production was obtained after 24 h of incubation at 37°C with an initial medium pH 8.0. Addition of surfactants like Tween 80 (0.25 g/L) and sodium lauryl sulfate (0.2 g/L) resulted in 28% and 15% increase in enzyme production, respectively. Amylase production was 3.06 times higher when optimized production conditions were used. Optimum reaction temperature and pH for crude amylase activity were 50°C and 6.0, respectively. The crude enzyme showed activity and stability over a fair range of temperature and pH. These results suggest that B. subtilis BI19 could be exploited for production of amylase at relatively low cost and time. © 2015 Biplab Kumar Dash et al.

Sen M.K.,Islamic University | Nasrin S.,National Institute of Biotechnology | Rahman S.,Jessore University of Science and Technology | Jamal A.H.M.,Islamic University
Asian Pacific Journal of Tropical Biomedicine | Year: 2014

Objective: To study callus induction from different explants (internode, leaf, root) and in vitro plantlets propagation from medicinally important plant Achyranthes aspera L. Methods: Sterilized explants were prepared by using 0.1% HgCl2 and 0.5% Bavistin and callus was obtained when cultured onto Murashige Skoog's (MS) medium by using different concentrations and combination of 2,4-D, NAA, BAP, IAA, IBA with 3% sucrose and 0.8% agar. Induced callus was immediately transferred to MS medium containing at different concentrations of phytohormones for shootlets and rootlets induction respectively. Results: Sterilization treatment of 0.1% HgCl2 for 2-3 min and Bavistin 0.5% for 10-12 min showed the highest percentage of asepsis and survival rate. Maximum induction of callus was obtained from a combination of 2.0 mg/L 2,4-D and 0.5 mg/L NAA from leaf. Highest shootlets number (4.83±0.17) and length (3.8±0.16) cm were observed on full strength MS medium when fortified with BAP 4.0 mg/L and KIN 0.5 mg/L. Concerted efforts of BAP 2.0 mg/L and NAA 0.5 mg/L on full strength MS medium showed highest leaf number (6.77±0.94). In vitro raised shoots were allowed to root on different strengths of MS medium fortified with IAA and IBA at different concentrations. Experimentally, 3.0 mg/L IBA was enabled to induce maximum rootlets number (10.0±9.82) on full strength MS medium. Afterwards, regenerated shoots with well developed roots were successfully subjected to hardening process and were acclimatized. The survived plantlets showed 66.67% survival frequency without any morphological abnormality. Conclusions: The results demonstrated that different explants were good source of callus induction, morphology analysis as well as indirect plantlets regeneration. © 2014 Asian Pacific Tropical Biomedical Magazine.

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