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Greater Noida, India

Singh M.,Indian Veterinary Research Institute | Singh D.K.,Indian Veterinary Research Institute | Shivaramu K.V.,Indian Veterinary Research Institute | Biswas R.,Indian Veterinary Research Institute | And 5 more authors.
Indian Journal of Animal Sciences | Year: 2010

In this study, an attempt was made to use PCR in diagnosis of sheep brucellosis using serum as sample; and the results were compared with those obtained in RBPT. Out of 36 samples tested 19 were found positive for brucellosis by RBPT. PCR detected 13 samples as positive.

Bisht A.,Indian Pharmacopoeia Commission | Singh S.,National Institute of Biologicals | Marwaha N.,Jawaharlal Institute of Postgraduate Medical Education & Research
Asian Journal of Transfusion Science | Year: 2013

A centralized hemovigilance program to assure patient safety and to promote public health has been launched for the first time in India on Dec 10, 2012 in 60 medical colleges in the first phase along with a well-structured program for monitoring adverse reactions associated with blood transfusion and blood product administration. National Institute of Biologicals (NIB) will be the National Coordinating Centre for Hemovigilance. This program will be implemented under overall ambit of Pharmacovigilance Program of India (PvPI), which is being coordinated by Indian Pharmacopoeia Commission (IPC). All medical colleges of the country will be enrolled in this program by the year 2016 in order to have a National Centre of Excellence for Hemovigilance at NIB, which will act as a global knowledge platform.

Grover S.S.,National Center for Disease control | Negi S.S.,National Center for Disease control | Negi S.S.,National Institute of Biologicals | Singh S.,National Center for Disease control | And 2 more authors.
Biologicals | Year: 2012

The level of circulating tetanus toxin, antitoxin and their individual influence on the outcome of tetanus cases were determined in unimmunized 125 neonatal and 39 infant cases of tetanus. PHA (passive haemagglutination) test showed 40% positive cases for toxin while its absence in the remaining cases indicated of either toxin fixation to the central nervous system (CNS) or it got neutralized by antitoxin. TN (toxin neutralization) and PHA test carried out in 46 sera samples revealed a strong positive correlation (r = 0.9) showing that 35/46 (76%) and 38/46 (82.6%) samples were positive for antitoxin, respectively. 25.4% of the neonate and infant cases and 34% of the control group had a protective serum tetanus antitoxin level. 42.5% of the paired sera from unimmunized mothers and their neonates showing nonprotective antitoxin levels suggested that a high level of antitoxin is needed for transplacental transfer, although transfer may not play a decisive role in the resistance against the disease. The presence of toxin or antitoxin in the clinical cases did not affect the outcome of the disease, although in neonates, presence of toxin was found to be a bad prognostic sign. This study explicitly advocates for the need to improve the vaccination coverage strategy. © 2012 The International Alliance for Biological Standardization.

Rajput M.K.,National Institute of Biologicals
Journal of Clinical and Diagnostic Research | Year: 2016

This review is confined to triplex nucleic acid testing (NAT) assays to be used on fully automated platform. Around the world, these assays are being used at various transfusion medicine centres or blood banks to screen blood units for HBV, HCV and HIV. These assay systems can screen up to 1000 blood units for HBV, HCV and HIV simultaneously in a day. This area has been dominated by mainly two manufacturers: M/s Gen-Probe-Novartis and M/s Roche Molecular Systems. The triplex NAT assay systems of both manufacturers are licensed by United States Food and Drug Administration. There is not much awareness about the technology and procedures used in these assays. The main objective of this review is to create awareness about the technology and procedure of these assays. © 2016, Journal of Clinical and Diagnostic Research. All rights reserved.

Rajput M.K.,National Institute of Biologicals | Rajput M.K.,Jawaharlal Nehru University
Genes and Genomics | Year: 2014

This review is confined to a handful and essential information on retrotransposons from various systems/hosts. The main objective of this review to explain that retrotransposons play a significant role in evolution by structuring and restructuring of genomes. The implications due to their structure and method of propagation makes them agents of genome evolution and biodiversity. The obesity of complex genomes is granted by diverse population of retrotransposons. The diversity at every level of biological organization including species, individual organism and molecules such as nucleic acid and proteins gives rise to evolution. The mechanism of regulation and activation of retrotransposons indicates that the retrotranaposons, which were earlier considered as junk, are very important for development of an organism and these elements play significant role in biological evolution. The similarity of retrotransposons with endoretroviruses brings them closer to retroviruses and helps to understand phylogenetics. The continued inheritance of retrotransposons may be due to evolutionary process that creates and preserves traits that are seemingly fitted for the functional roles they perform. © 2014, The Genetics Society of Korea and Springer-Science and Media.

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