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Hassan M.N.,Bangladesh Agricultural University | Rahman M.,Bangladesh Agricultural University | Hossain M.B.,Noakhali Science and Technology University | Hossain M.B.,Biology Group | And 3 more authors.
Egyptian Journal of Aquatic Research | Year: 2013

Presence of nitrofuran and chloramphenicol in farmed prawn and shrimp is a major concern in the export sector of Bangladesh. Rejections of consignments by the foreign buyers have been recurrent for the last couple of years due to detection of these banned antibiotics. The increasingly complex requirements for food safety assurance and traceability set by major export markets represent a threat to the trade of this significant sector. In this study, the status and trends of the presence of nitrofuran and chloramphenicol in freshwater prawn Macrobrachium rosenbergii (De Man) and marine shrimp Penaeus monodon (Fabricius) and in their feeds in the Southwest coastal region of Bangladesh were investigated. The prawn/shrimp farmers, feed manufacturers and feed sellers were interviewed with well structured pre-tested Questionnaires. Antibiotic residues present in the animal muscles, feeds and feed ingredients were detected using LC-MS-MS by the Fish Inspection and Quality Control Wing of the Department of Fisheries. The study reveals that farmers did not deliberately use those banned antibiotics, but these chemicals were detected in many M. rosenbergii and P. monodon samples in 2008, 2009 and 2010, in both fresh muscles, pre-export and post-export consignments. The percentage of contamination with this banned antibiotics in M. rosenbergii samples were 17.74%, 22.89%, 13.60% and in P. monodon samples were 12.65%, 15.79%, 11.85% in 2008, 2009 and 2010 respectively. Antibiotic residue data of 2008, 2009 and 2010 showed: (i) contamination of nitrofuran and chloramphenicol was more in M. rosenbergii than P. monodon; (ii) among four nitrofuran metabolites, nitrofurazone was found more frequently but in smaller quantities in 2009 and 2010; (iii) contamination of nitrofuran metabolites was more in prawns fed with commercial feed than those with home-made feed; and (iv) there has been a decreasing trend of the presence of nitrofuran and chloramphenicol in prawn and shrimp of Bangladesh. © 2013 .

Teixeira B.,National Institute Of Biological Resources Inrb Ip L Ipimar | Teixeira B.,University of Aveiro | Marques A.,National Institute Of Biological Resources Inrb Ip L Ipimar | Ramos C.,National Institute Of Biological Resources Inrb Ip L Ipimar | And 7 more authors.
Industrial Crops and Products | Year: 2012

There is a growing interest of industry to replace synthetic chemicals by natural products with bioactive properties from plant origin. The aim of this study was to determine the chemical composition of European pennyroyal (Mentha pulegium) essential oil and to characterize the in vitro antioxidant and antimicrobial activities of its water (hot and cold) and ethanolic extracts and of the essential oil. The essential oil revealed menthone, pulegone and neo-menthol as the main constituents, comprising 35.9, 23.2 and 9.2% of the essential oil, respectively. The hot water extract exhibited the highest antioxidant activity and phenol content. In contrast, the extracts were not very effective to inhibit the growth of the seven foodborne spoilage and pathogenic bacteria tested, but the essential oil showed antibacterial activity against all bacterial strains. In conclusion, extracts and essential oil of M. pulegium from Mediterranean origin have huge potential as an alternative to chemical additives for the food industry. © 2011 Elsevier B.V.

Pereira C.,University of Aveiro | Santos L.,University of Aveiro | Silva A.P.,University of Aveiro | Silva Y.J.,University of Aveiro | And 4 more authors.
Marine Pollution Bulletin | Year: 2015

The recurrent emergence of infections outbreaks associated with shellfish consumption is an important health problem, which results in substantial economic losses to the seafood industry. Even after depuration, shellfish is still involved in outbreaks caused by pathogenic bacteria, which increases the demand for new efficient strategies to control the shellfish infection transmission. Phage therapy during the shellfish depuration is a promising approach, but its success depends on a detailed understanding of the dynamics of bacterial communities in the harvesting waters. This study intends to evaluate the seasonal dynamics of the overall bacterial communities, disease-causing bacterial populations and bacterial sanitary quality indicators in two authorized harvesting-zones at Ria de Aveiro.During the hot season, the total bacterial community presented high complexity and new prevalent populations of the main shellfish pathogenic bacteria emerged. These results indicate that the spring/summer season is a critical period during which phage therapy should be applied. © 2014 Elsevier Ltd.

Barrento S.,National Institute Of Biological Resources Inrb Ip L Ipimar | Barrento S.,University of Porto | Marques A.,National Institute Of Biological Resources Inrb Ip L Ipimar | Vaz-Pires P.,University of Porto | Nunes M.L.,National Institute Of Biological Resources Inrb Ip L Ipimar
Aquaculture Research | Year: 2012

The trade of live Cancer pagurus in Portugal is totally dependent on imports, mainly from UK. Animals are transported for 48-58 h in conditions that promote high mortalities (40-60%) due to cumulative stressors. The present work aimed to optimize transport, by comparing the physiological responses of edible crab during different simulated live shipment systems for 48 h and recovery conditions: (a) immersed crabs at 12°C; and (b) air-exposed crabs at 4°C and subsequent recovery at 16°C with and without pre-acclimation. Both simulated transport systems induced physiological stress, culminating in 25% mortality in immersed conditions and only 4% in air-exposed conditions. Both systems elicited anaerobic responses marked by hyperglycaemia and increased l-lactate concentration in haemolymph. At the end of the recovery period only air-exposed animals resumed to l-lactate and pH control values, the lowest mortality was achieved with crabs acclimated at the lowest temperature (from 6 to 16°C at 1.5°C/h). Overall, results showed that crabs can be transported in air-exposed conditions at 4°C and acclimated from 6 to 16°C and reach similar physiological conditions than the traditional transport in immersed conditions at 12°C. © 2011 Blackwell Publishing Ltd.

Oliveira H.,National Institute Of Biological Resources Inrb Ip L Ipimar | Oliveira H.,Polytechnic Institute of Coimbra | Oliveira H.,University of Porto | Oliveira H.,The Interdisciplinary Center | And 5 more authors.
Comprehensive Reviews in Food Science and Food Safety | Year: 2012

The main objective of this review is to summarize the present state of knowledge of different ways of processing cod, giving emphasis to salting, drying, and desalting steps. As an introduction, a description of the main characteristics of the Gadidae family and cod species, the general chemical composition of fresh cod, and a reference to farmed cod is included. Statistics on world fishery and aquaculture cod production are also reviewed. It is expected that this review will contribute to build a current picture of the technical and scientific knowledge on the cod processing situation, helping to move forward and to support future developments in this important seafood industry. © 2012 Institute of Food Technologists®.

Barrento S.,National Institute Of Biological Resources Inrb Ip L Ipimar | Marques A.,National Institute Of Biological Resources Inrb Ip L Ipimar | Teixeira B.,National Institute Of Biological Resources Inrb Ip L Ipimar | Mendes R.,National Institute Of Biological Resources Inrb Ip L Ipimar | And 3 more authors.
Journal of Food Composition and Analysis | Year: 2010

The brown crab, Cancer pagurus, is a valued decapod species captured mainly in the UK and France. In Portugal, Scottish crabs and females of all species are less expensive than crabs caught in the English Channel and males. In this work the proximate chemical composition, cholesterol, fatty acid and amino acid content of female and male C. pagurus edible tissues from the Scottish coast and English Channel were compared. There was no evidence that the fishing ground influenced the chemical composition of tissues, but there were significant differences between tissues and sexes. Muscle was richer in protein, but poorer in fat and cholesterol, than gonads and hepatopancreas. Ovaries had more protein, fat, cholesterol and amino acids than testes. The fatty acid profiles in muscle and gonads were dominated by PUFA, while hepatopancreas was richer in MUFA and SFA. Lower n-3 fatty acid content and n-3/. n-6 fatty acid ratio in hepatopancreas contributed to higher atherogenic and thrombogenic indices. Considering the chemical composition, there is no reason for price differentiation between crabs from different locations. As far as sex is concerned the principal difference that might increase male crabs' value is the meat yield content of claws, which was higher than females' claws. © 2010 Elsevier Inc.

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