National Institute of Agricultural Biotechnology

Suigen, South Korea

National Institute of Agricultural Biotechnology

Suigen, South Korea

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Kim S.-Y.,Sangji University | Lee B.-M.,National Institute of Agricultural Biotechnology | Cho J.-Y.,Sangji University
Biotechnology Letters | Year: 2010

Two genes involved in central carbon metabolism were inactivated to modulate intracellular glucose 6-phosphate and to evaluate its effects on xanthan production in wild-type Xanthomonas oryzae pv. oryzae. Upon the inactivation of the phosphogluconate dehydratase gene (edd), intracellular glucose 6-phosphate increased from 0.05 to 1.17 mmol/g (dry cell wt). This was accompanied by increased xanthan production of up to 2.55 g/l (culture medium). In contrast, inactivation of 6-phosphogluconate dehydrogenase gene (gndA) did not influence intracellular glucose 6-phosphate nor xanthan production. The intracellular availability of glucose 6-phosphate is proposed as a rate-limiting factor in xanthan production, and it may be possible to increases production of xanthan by modulating the activities of enzymes in central carbon metabolism. © Springer Science+Business Media B.V. 2009.


Jang S.-G.,Sangji University | Jang S.-G.,National Institute of Agricultural Biotechnology | Lee B.-M.,National Institute of Agricultural Biotechnology | Cho J.-Y.,Sangji University
Journal of Industrial Microbiology and Biotechnology | Year: 2012

In this study, the glucose 6-phosphate dehydrogenase gene (XOO2314) was inactivated in order to modulate the intracellular glucose 6-phosphate, and its eVects on xanthan production in a wild-type strain of Xanthomonas oryzae were evaluated. The intracellular glucose 6-phosphate was increased from 17.6 to 99.4 μmol g -1 (dry cell weight) in the gene-disrupted mutant strain. The concomitant increase in the glucose 6-phosphate was accompanied by an increase in xanthan production of up to 2.23 g l -1 (culture medium). However, in deWned medium supplemented with 0.4% glucose, the growth rate of the mutant strain was reduced to 52.9% of the wild-type level. Subsequently, when a family B ATP-dependent phosphofructokinase from Escherichia coli was overexpressed in the mutant strain, the growth rate was increased to 142.9%, whereas the yields of xanthan per mole of glucose remained approximately the same. © Society for Industrial Microbiology and Biotechnology 2011.


Madhaiyan M.,Chungbuk National University | Madhaiyan M.,National University of Singapore | Poonguzhali S.,Chungbuk National University | Kwon S.-W.,National Institute of Agricultural Biotechnology | Sa T.-M.,Chungbuk National University
International Journal of Systematic and Evolutionary Microbiology | Year: 2010

A Gram-positive bacterium, designated strain CBMB205 T, was isolated from the rhizosphere soil of traditionally cultivated, field-grown rice. Cells were strictly aerobic, motile, rod-shaped and formed endospores. The best growth was achieved at 30 °C and pH 7.0 in ammonium mineral salts (AMS) medium containing 600 mM methanol. A comparative 16S rRNA gene sequence-based phylogenetic analysis placed strain CBMB205 T in a clade with the species Bacillus amyloliquefaciens, Bacillus vallismortis, Bacillus subtilis, Bacillus atrophaeus, Bacillus mojavensis and Bacillus licheniformis and revealed pairwise similarities ranging from 98.2 to 99.2 %. DNA-DNA hybridization experiments revealed a low level (<36 %) of DNA-DNA relatedness between strain CBMB205 T and its closest relatives. The major components of the fatty acid profile were C 15 : 0 anteiso, C 15 : 0 iso, C 16 : 0 iso and C 17 : 0 anteiso. The diagnostic diamino acid of the cell wall was meso-diaminopimelic acid. The G+C content of the genomic DNA was 45.0 mol%. The lipids present in strain CBMB205 T were diphosphatidylglycerol, phosphatidylglycerol, a minor amount of phosphatidylcholine and two unknown phospholipids. The predominant respiratory quinone was MK-7. Studies of DNA-DNA relatedness, morphological, physiological and chemotaxonomic analyses and phylogenetic data based on 16S rRNA gene sequencing enabled strain CBMB205 T to be described as representing a novel species of the genus Bacillus, for which the name Bacillus methylotrophicus sp. nov. is proposed. The type strain is CBMB205 T (=KACC 13105 T=NCCB 100236 T). © 2010 IUMS.


Zhao W.-G.,Kongju National University | Zhao W.-G.,Chinese Academy of Agricultural Sciences | Chung J.-W.,Kongju National University | Cho Y.-I.,Kongju National University | And 8 more authors.
Comptes Rendus - Biologies | Year: 2010

This study was conducted to assess the genetic diversity and population structure of 139 Lycium chinense accessions using 18 simple sequence repeat (SSR) markers. In total, 108 alleles were detected. The number of alleles per marker locus ranged from two to 17, with an average of six. The gene diversity and polymorphism information content value averaged 0.3792 and 0.3296, with ranges of 0.0793 to 0.8023 and 0.0775 to 0.7734, respectively. The average heterozygosity was 0.4394. The model-based structure analysis revealed the presence of three subpopulations, which was consistent with clustering based on genetic distance. An AMOVA analysis showed that the between-population component of genetic variance was less than 15.3%, in contrast to 84.7% for the within-population component. The overall FST value was 0.1178, indicating a moderate differentiation among groups. The results could be used for future L. chinense allele mining, association mapping, gene cloning, germplasm conservation, and designing effective breeding programs. © 2010 Académie des sciences. Published by Elsevier Masson SAS. All rights reserved.


Cho Y.-G.,National Institute of Agricultural Biotechnology | Cho Y.-G.,Chungbuk National University | Kang H.-J.,Honam Agricultural Research Institute | Lee Y.-T.,Honam Agricultural Research Institute | And 3 more authors.
Plant Biotechnology Reports | Year: 2010

Quantitative trait loci (QTL) associated with six physical traits of cooked rice and seven chemical properties of rice grain were identified using a recombinant inbred (RI) population of rice evaluated over 3 years at the National Honam Agricultural Research Institute in Korea. The RI population consisted of 164 lines derived from a cross between Milyang23 and Gihobyeo, and the genetic map consisted of 414 molecular markers. A total of 49 QTL were identified for the 13 physico-chemical properties using composite interval mapping. Of these, 13 QTL were identified for 2 or more years, while 36 were detected in only 1 year. Five QTL were identified over all 3 years and will be useful for marker-assisted improvement of rice grain quality in Korea. The two QTL with the highest LOD scores, adhesiveness1. 2 and potassium content7. 1, provide a valuable starting point for positional cloning of genes underlying these QTL. © Korean Society for Plant Biotechnology and Springer 2010.


Cho Y.-I.,Kongju National University | Chung J.-W.,Kongju National University | Lee G.-A.,National Institute of Agricultural Biotechnology | Ma K.-H.,National Institute of Agricultural Biotechnology | And 3 more authors.
Genes and Genomics | Year: 2010

Millets such as proso millet have excellent nutritional properties and could become a basic resource for crop breeding programs and food diversification. In this study, 25 polymorphic microsatellite markers were developed and characterized through construction of an SSR-enriched library from genomic DNA of proso millet (Panicum miliaceum L.). In total, 110 alleles were detected, with an average of 4.4 alleles per locus. Values of major allele frequency (M AF ) and expected heterozygosity (H E ) ranged from 0.36 to 0.98 (mean = 0.73) and from 0.04 to 0.74 (mean = 0.37), respectively. The mean genetic similarity coefficient was 0.3711, indicating that among 50 accessions of proso millet there was wide genetic variation. The newly developed microsatellite markers should be useful tools for assessing genetic diversity, understanding population structure, and breeding of proso millet. © 2010 The Genetics Society of Korea and Springer Netherlands.


Khaing A.A.,Kongju National University | Moe K.T.,Kongju National University | Chung J.-W.,National Institute of Agricultural Biotechnology | Baek H.-J.,National Institute of Agricultural Biotechnology | Park Y.-J.,Kongju National University
Plant Breeding | Year: 2013

The genus Amaranthus has gained much attention, particularly for its high economic and nutritional value. It is a genus of taxonomic complexity with many interspecific hybrids. For effective conservation and management of the germplasm collected, the development of a core set of accessions is especially important. A core set of 63 accessions was successfully developed from an entire collection of 634 accessions using the powercore 1.0. Among the tested methods for developing the core set - the advanced M strategy using the modified heuristic method (HCC), randomly chosen collections and stratified random collections - HCC was found to be best, with 100% coverage of alleles and minimum redundancy. Allele frequency distribution of the core set developed with HCC was highly correlated with that of entire collections (r = 0.91). Model-based structure analysis revealed the presence of three subpopulations and 11 admixtures in the selected core set, which is basically consistent with clustering based on the genetic distance. The results from this study will provide effective information for future germplasm conservation and improvement programmes in Amaranthus. © 2013 Blackwell Verlag GmbH.


Shin K.-S.,National Institute of Agricultural Biotechnology | Park S.-Y.,Chungbuk National University | Paek K.-Y.,Chungbuk National University
In Vitro Cellular and Developmental Biology - Plant | Year: 2013

Suboptimal environmental conditions inside closed culture vessels can be detrimental to in vitro growth and survival of plantlets during the acclimatization process. In this study, the environmental factors that affected Doritaenopsis plantlet growth and the relationship between growth and sugar metabolism were investigated. Cultures were maintained under heterotrophic, photoautotrophic, or photomixotrophic conditions under different light intensities and CO2 concentrations. Photoautotrophic growth of Doritaenopsis hybrid plantlets could be promoted significantly by increasing the light intensity and CO2 concentration in the culture vessel. The concentration of different sugars in the leaves of in vitro-grown plantlets varied with different cultural treatments through a 10-wk culture period. Starch, reducing sugars, and nonreducing sugar contents were higher in plantlets grown under photoautotrophic and photomixotrophic conditions than in heterotrophically grown plantlets. Net photosynthesis rates were also higher in photoautotrophically and photomixotrophically grown plantlets. These results support the hypothesis that pyruvate, produced by the decarboxylation of malate, is required for optimal photoautotrophy under high photosynthetic photon flux density. Growth was greatest in plantlets grown under CO2-enriched photoautotrophic and photomixotrophic conditions with high photosynthetic photon flux density. The physiological status of in vitro-grown Crassulacean acid metabolism (CAM)-type Doritaenopsis showed a transition from C3 to CAM prior to acclimatization. © 2013 The Society for In Vitro Biology.


Verma N.,National Bureau of Plant Genetic Resources | Rana M.K.,National Bureau of Plant Genetic Resources | Negi K.S.,National Bureau of Plant Genetic Resources | Kumar G.,National Bureau of Plant Genetic Resources | And 3 more authors.
Indian Journal of Biotechnology | Year: 2010

Inter-population diversity in 54 Indian Perilla landraces and 18 accessions of exotic origin was investigated using sequence tagged microsatallite (STMS) markers. The STMS markers clearly distinguished the Indian accessions from the exotic ones. Neighborhood-joining (NJ) clustering pattern revealed association of geographical diversity and genetic diversity for Indian Perilla germplasm. Population genetic parameters studied for 14 Indian Perilla populations from two distinct regions (North-eastern region and North-western Himalayas, Uttarakhand State) revealed greater diversity for accessions from the later region. Analysis of molecular variance, revealed that bulk of the variations existed between populations within groups, followed by variations within populations and between groups. The summary of group-wise F-statistics and gene flow revealed greater population differentiation for Uttarakhand populations as compared to accessions from North-eastern region of India.


PubMed | National Institute of Agricultural Biotechnology
Type: Journal Article | Journal: Mycobiology | Year: 2013

In this study, a total of 300 isolates of Penicillium and related teleomorphic genera were collected from soils of 17 locations in Korea from April to May, 2004. Ninety four isolates were identified as the species of Penicillium subgenus Furcatum based on cultural and morphological characteristics and -tubulin gene sequences. Among the species, Korean isolates of P. brasilianum Bat. and P. daleae K. M. Zalessky were phylogenetically identical to the reference species based on DNA sequence of the -tubulin gene. Here we described and illustrated P. brasilianum and P. daleae that are new in Korea.

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