National Institute Mt Kassab Of Orthopedic

Manouba, Tunisia

National Institute Mt Kassab Of Orthopedic

Manouba, Tunisia
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Hamza S.,National Institute Mt Kassab Of Orthopedic | Hamza S.,Tunisian National Institute of Applied Sciences and Technology | Bouchemi M.,Tunisian National Institute of Applied Sciences and Technology | Slimane N.,National Institute Mt Kassab Of Orthopedic | Azari Z.,Laboratory of Biomechanics
Materials Science and Engineering C | Year: 2013

Bone substitutes are more and more used in bone surgery because of their biologic safety, clinic efficiency and facility to synthesize. Bone substitutes with active osteogenic properties, associating biomaterials with organic macromolecule components of the extracellular matrix (protein, GAG) are recommended. Nevertheless, we should have a simple technique to control interactions between proteins and the material. Natural coral and nacre have been found to be impressive bone graft substitutes. In this work, we characterize nacre and coral powder using energy dispersive X-ray analysis (EDX). We used electrochemical impedance spectroscopy (EIS) and attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy to evaluate bovine serum albumin (BSA) as model protein, adsorbed to these biomaterial surfaces. In order to understand the nacre/coral-protein interfacial compatibility, it is necessary to investigate the wettability. © 2012 Elsevier B.V.


Hamza S.,National Institute Mt Kassab Of Orthopedic | Hamza S.,Tunisian National Institute of Applied Sciences and Technology | Slimane N.,National Institute Mt Kassab Of Orthopedic | Azari Z.,Laboratory of Biomechanics | Pluvinage G.,Laboratory of Biomechanics
Applied Surface Science | Year: 2013

The main objective of this work is to develop resistant compact material samples with different porosities from coral and nacre adapted to the filling of bone cavities. The characterization of materials was conducted using scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and laser granulometry. The micro-hardness and the influence of porosity on the mechanical behavior of these biomaterials under compression as well as three-points bending tests were also assessed. Both materials showed similar particles size ranging from 50 to 100 μm in diameter, distributed according to the Gauss curve. The modal particle size, the median D50 and D 90-D10 are the most important parameters which allow for the distinction between coral and nacre samples. The two biomaterials showed a micro hardness (138-167 HV for coral and 261-340 HV for nacre) higher than that of bovine bones (55-70 HV). The maximum compression stresses were 32.82 MPa for coral and 37.06 MPa for nacre at 50% of porosity. S-N curve with ASME format is constructed to predict the fatigue life extended from 101 to 10 6 cycles, which reveals an endurance limit at a compression stress ratio of about 10. © 2012 Elsevier B.V. All rights reserved.

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