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Iezhov V.V.,Si Georgievsky Crimean State Medical University | Mizin V.I.,Crimean State Humanitarian University | Yalaneckyy A.Y.,National Institute for Vine and Wine Magarach
NATO Science for Peace and Security Series A: Chemistry and Biology

As was established in previous investigations, consumption of grape's polyphenols, containing in food concentrate Enoant, increase effectiveness of recuperative sanitaria treatment in patients with arterial hypertension and chronic bronchitis. In current article, we present the results of investigation of the wine stress-limiting effects caused by alcohol and polyphenol components. The investigation was performed in 60 patients with arterial hypertension, 60 patients with chronic bronchitis and 60 patients with chronic fatigue syndrome. White table dry wine was used under complex recuperative sanitaria treatment in patients with arterial hypertension and chronic bronchitis and the Ukrainian sparkling wine was used under complex recuperative spa treatment in patients with chronic fatigue syndrome. The dry wine was prepared from white grape "Rkacitely" along with Kakhetian technology. The analysis of data reveals that the most positive wine effect is a successful coping with stress. We had seen significantly increased changes in psychological and morphological reactions, including positive dynamics of the following: patient's self indicated feeling, amount of total complaints and stress reactions, adaptation, diastolic blood pressure, minute blood volume, right carotid artery rheographic index and Gentch test. Also, about quarter of patients had noted the diminishing of wish to drink strong alcohol beverages. The results are in good accordance with the previously known action of grape's wines to prevent heart and vessels diseases. © 2013 Springer Science+Business Media Dordrecht. Source

Goryslavets S.,National Institute for Vine and Wine Magarach | Risovanna V.,National Institute for Vine and Wine Magarach | Bacilieri R.,French National Institute for Agricultural Research | Hausman J.-F.,Center De Recherche Public Gabriel Lippmann | And 3 more authors.
Cytology and Genetics

Four bred grapevine varieties released for commercial cultivation in Ukraine, namely 'Antey Magarachskii', 'Rubinovyi Magaracha', 'Granatovyi Magaracha' and 'Rubin Golodrigi', and their putative parental forms were genotyped using six microsatellite loci. Genotypes were compared with breeding records to verify genetic relationships among varieties. Results of the analysis confirmed four of six parent-offspring relationships. Results of the analysis allow to assume that genotype 'Seyve Villard 20347' is the direct parent of 'Antey Magarachskii' instead of its grandparent. The first-studied accession believed to be that of 'Granatovyi Magaracha' was identified as impurity. In order to verify the parentage of 'Granatovyi Magaracha', rest accessions of that variety and its putative parent 'Antey Magarachskii' were additionally genotyped at 13 nuclear loci and at three chloroplast loci. The parent-offspring relationship was confirmed, as all 'Granatovyi Magaracha' accessions had a common allele with the parent variety 'Antey Magarachskii' at each locus and the same chlorotype A. Different 'Granatovyi Magaracha' accessions could have been obtained via vegetative propagation of two seedlings which arose from one crossing. © Allerton Press, Inc., 2010. Source

Levchenko S.V.,National Institute for Vine and Wine Magarach | Volynkin V.A.,National Institute for Vine and Wine Magarach
Acta Horticulturae

Aroma-forming components in the varietal musts of three newly-bred cultivars, 'Tsitronnyi Magaracha', 'Spartanets Magaracha', and the elite form 'Muscat JIM', and five hybrid seedlings resulting from crosses with at least one of them as a parent and having different intensities of muscat aroma were identified by gas chromatography. Terpene compounds, unsaturated alcohols and aldehydes concentrations were identified. Linalool, its oxides and isomers, and limonene were the major terpene compounds. The chromatography also revealed the presence of substances masking the muscat aroma. Source

Mizin V.I.,National Institute for Vine and Wine Magarach | Ogay Y.A.,National Institute for Vine and Wine Magarach
NATO Science for Peace and Security Series C: Environmental Security

Modern medicine pays a great attention to environmental security, i.e. to elimination of adverse health effects due to environmental factors. From the end of twentieth century, the environment became a source of urban and industry induced threats, such as: air and water microbe and chemical pollution, increased levels of ionizing radiation, and high risk of traumas. Essentially, the food, as very important environmental factor, has also changed in the modern era due to new agriculture technologies, refining, synthetic food additives, the influences of ultra-high and ultra-low temperatures, gene engineering, etc. On the other hand, one of the important achievements of the modern medicine is an introduction of functional foods for maintenance of homeostasis of human metabolism under the current environmental conditions. Imbalance between free radicals in organism induced by a variety of stresses and dietary antioxidants is one of the factors damaging human health. Bioantioxidants, i.e. antioxidants naturally engaged in metabolism, are of special value for medical purposes of prevention, treatment and rehabilitation. The adverse effects related to the bioantioxidant deficiency are generated by the accumulation of products arising from oxidation induced by free radicals. An increased need for bioantioxidants may emerge under specific conditions: (1) stress, (2) exposure to ionizing radiation, xenobiotics and another harmful man-made factors of the environment, (3) too much or too little physical activity due to traumas and rescue efforts, (4) administration of pharmaceuticals activating processes of oxidation induced by free radicals, such as antibiotics, narcotic and non-narcotic analgesics, and non-steroid anti-inflammatory preparations. The alcohol-free dietary concentrate Enoant developed by the National Institute for Vine and Wine "Magarach" represents a practical approach to use the Cabernet Sauvignon grape for prevention and treatment of various maladies. Experimental and clinical studies have shown curative and preventive benefits of Enoant as a part of methodology used in catastrophe medicine. These may contribute to the achievement, with better effectiveness, of clinical goals in complex treatment and rehabilitation of humans, possessing impaired functions of the cardio-respiratory system, red blood cells, the antioxidant system and lipid metabolism by improving the immunity and lessening of toxic side effects of radiation and pharmacotherapy. Data from experiments and clinical studies on effects exerted by Enoant indicate that the criteria for optimization of medical technologies should include both a sufficient daily dose of the concentrate and the absence of individual negative reactions or grape-induced allergy. Source

Goryslavets S.,National Institute for Vine and Wine Magarach | Bacilieri R.,Montpellier SupAgro | Risovannaya V.,National Institute for Vine and Wine Magarach | Memetova E.,National Institute for Vine and Wine Magarach | Laucou V.,Montpellier SupAgro
Vitis - Journal of Grapevine Research

A total of 76 accessions of Crimean autochthonous grape cultivars from the collection of the National Institute of Vine and Wine "Magarach" were genotyped using 22 nuclear and 3 chloroplast microsatellite loci (SSR) to characterize their genetic diversity. The total number of alleles was 238, the mean number of alleles per locus was 10.8 and the range of expected heterozygosity was 0.4-0.88. Several synonyms were identified based on the comparison of microsatellite profiles with INRA and EVD databases. Morphological, eno-carpological and eno-chemical characterization of number of cultivars using BBCH scale and OIV descriptors were performed. A short synopsis of the origin and historical development of Crimean autochthonous grape cultivars is presented. Source

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