Radu N.,Romanian National Institute for Research and Development in Chemistry and Petrochemistry |
Salageanu A.,National Institute for Research and Development in Microbiology and Immunology |
Tanasescu C.,Lucian Blaga University
Molecular Crystals and Liquid Crystals | Year: 2016
The processes of regeneration and repair tissues that occur under action of any substance are based on the effects determinated by this substance on some soluble mediators (cytokines, growth factors, pro-/anti-inflammatory agents) and on the macrophages involved in process. In this respect in this paper we will present an in vitro study of three kinds of biomaterials (P1, P2 and P3) obtained by submerged biosynthesis. These materials were synthesised using three strains of Monascus sp. The biocompatibility studies, performed with the three biomaterials on the adherent cells lines, type L929 murine fibroblast or RAW 264.7 murine macrophage, showed that only the biomaterial P1 is biocompatible. In the presence of biomaterial P2 or P3, the cell viability is under 70% and it is not depending on their concentration, meaning that these biomaterials are not biocompatible. In order to evaluate P1 biomaterial capacity to activate secretion of mediators involved in the process of tissue repair, for this biomaterial was determined the ability of inducing the secretion of VEGF (vascular endothelial growth factor) for the human monocyte THP-1 cells line. The results obtained in vitro have been confirmed that in the presence of biomaterial P1, the macrophages THP-1 adopt a profile associated with accelerating the process of tissue repair characterized by the absence of the secretion of pro-inflammatory cytokines (TNF-α, IL-6) respectively by the secretion of anti-inflammatory cytokines (IL-1RA). © 2016 Taylor & Francis Group, LLC.
Stavri H.,National Institute for Research and Development in Microbiology and Immunology |
Bucurenci N.,National Institute for Research and Development in Microbiology and Immunology |
Ulea I.,National Institute for Research and Development in Microbiology and Immunology |
Costache A.,National Institute for Research and Development in Microbiology and Immunology |
And 2 more authors.
Indian Journal of Medical Research | Year: 2012
Background & objectives: Purified protein derivative (PPD) is currently the only available skin test reagent used worldwide for the diagnosis of tuberculosis (TB). The aim of this study was to develop a Mycobacterium tuberculosis specific skin test reagent, without false positive results due to Bacillus Calmette-Guerin (BCG) vaccination using recombinant antigens. Methods: Proteins in PPD IC-65 were analyzed by tandem mass spectrometry and compared to proteins in M. tuberculosis culture filtrate; 54 proteins were found in common. Top candidates MPT64, ESAT 6, and CFP 10 were overexpressed in Escherichia coli expression strains and purified as recombinant proteins. To formulate optimal immunodiagnostic PPD cocktails, the antigens were evaluated by skin testing guinea pigs sensitized with M. tuberculosis H37Rv and BCG. Results: For single antigens and a cocktail mixture of these antigens, best results were obtained using 3 μg/0.1 ml, equivalent to 105 TU (tuberculin units). Each animal was simultaneously tested with PPD IC65, 2 TU/0.1 ml, as reference. Reactivity of the multi-antigen cocktail was greater than that of any single antigen. The skin test results were between 34.3 and 76.6 per cent the level of reactivity compared to that of the reference when single antigens were tested and 124 per cent the level of reactivity compared to the reference for the multi-antigen cocktail. Interpretation & conclusions: Our results showed that this specific cocktail could represent a potential candidate for a new skin diagnostic test for TB.