National Institute For Digestive Diseases Of Bellis

Castellana Grotte, Italy

National Institute For Digestive Diseases Of Bellis

Castellana Grotte, Italy
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Antoniotti S.,University of Turin | Ruffinatti F.A.,University of Turin | Ruffinatti F.A.,University of Piemonte Orientale | Torriano S.,University of Turin | And 5 more authors.
Neuroscience Letters | Year: 2016

The repressor element-1 silencing transcription factor (REST) has emerged as a key controller of neuronal differentiation and has been shown to play a critical role in the expression of the neuronal phenotype; however, much has still to be learned about its role at specific developmental stages and about the functional targets affected. Among these targets, calcium signaling mechanisms are critically dependent on the developmental stage and their full expression is a hallmark of the mature, functional neuron. We have analyzed the role played by REST in GN11 cells, an immortalized cell line derived from gonadotropin hormone releasing hormone (GnRH) neurons at an early developmental stage, electrically non-excitable and with a strong migratory activity. We show for the first time that functional voltage-dependent calcium channels are expressed in wild type GN11 cells; down-regulation of REST by a silencing approach shifts these cells towards a more differentiated phenotype, increasing the functional expression of P/Q-type channels and reducing their migratory potential. © 2016 Elsevier Ireland Ltd

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