National Institute for Chemical Pharmaceutical R and D ICCF Bucharest

Bucharest, Romania

National Institute for Chemical Pharmaceutical R and D ICCF Bucharest

Bucharest, Romania
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Grigore A.,National Institute for Chemical Pharmaceutical R and D ICCF Bucharest | Ionita C.,National Institute for Chemical Pharmaceutical R and D ICCF Bucharest
Romanian Biotechnological Letters | Year: 2013

Pharmacological activity of Eupatorium cannabinum L. hydroalcoholic extract have been studied with the use of two in vitro assays - DPPH reduction spectrophotometric assay, and evaluation of cytotoxic effect on Jurkat cells by MTS assay. Also, HPTLC fingerprint and HPLC analysis were performed in order to elucidate the chemical composition of the extract. Thus, it was found that the extract contains 56.86mg% caffeic acid, 1.26mg% eupatorin, 18.06mg% eupatilin, 97.03mg% quercetin, 762.63mg% rutin, 68.83mg% β-ecdysone, among other unidentified compounds. It was showed the dose-response dependency in the DPPH assay, the extract exhibiting an inhibition of DPPH radical of 59% at 3mg/ mL concentration, comparing to caffeic acid (0.1mg/ mL) used as standard. In the cytotoxicity assay on Jurkat cells, the extract (7- 500 μg/mL) showed a dose-response cytotoxic effect, both at 24 and 48 hours. Moreover the single-dose toxicity test proved that the extract is non-toxic at the dose of 125mg/ 20gw and it is safe to use. This study contributes to the knowledge of E. cannabinum, add a scientific proof of the known traditional uses and, in the same time, opens the way for further investigations. © 2013 University of Bucharest.


George B.,University of Bucharest | Corina B.,National Institute for Chemical Pharmaceutical R and D ICCF Bucharest | Gheorghe C.,University of Bucharest
Romanian Biotechnological Letters | Year: 2015

Oxidative stress, that generates reactive oxygen and nitrogen species, is caused by an imbalance occurred at the cellular level and causes the appearance of several disease, including atherosclerosis, diabetes, neurodegenerative diseases and cancer. We investigated by two methods: DPPH and total antioxidant capacity, antioxidant capacity and free radical scavenger potential of three hydroalcoholic extracts from: Humulus lupulus (cones) and Vaccinium myrtillus (leaves) and a combined extract obtained as a mixture of the two species, compared with the reference substances chlorogenic acid and rutin, highlighting the chemical composition of the extracts in total polyphenolic compounds. Reference substances have a similar behavior in both test methods, meanwhile the studied samples have opposite behaviors. In DPPH assay Vaccinium myrtillus extract has a higher scavenger capacity than Humulus lupus extract at the most concentrations and test times leading to a direct dependence between the inhibition potential of DPPH and composition in total polyphenolic compounds. In phosphomolybdenum assay Humulus lupulus extract has total antioxidant capacity higher than the Vaccinium myrtillus extract, at all tested concentrations, which may be due to the different reactivity of the extracts compounds with phosphomolybdic reagent, taking into account both time and reaction temperature, as well as the chemical complexity of the extracts. Antioxidant activity of combined extract tends to achieve a balance compared with the results for individual extracts, in the determinations made by the two methods, including in the case of quantitative chemical composition. © 2015 University of Bucharest.


Bubueanu C.,National Institute for Chemical Pharmaceutical R and D ICCF Bucharest | Gheorghe C.,University of Bucharest | Pirvu L.,National Institute for Chemical Pharmaceutical R and D ICCF Bucharest | Bubueanu G.,Horia Hulubei National Institute of Physics and Nuclear Engineering
Romanian Biotechnological Letters | Year: 2013

Antioxidant effect of Lamium album and Lamium purpureum butanol extracts was estimated using two methods: DPPH and chemiluminescence. Quantitative determination of phenolic compounds (Folin- Ciocalteu) was made in order to establish a correlation between the chemical composition of the extracts and antioxidant potential. The results showed strong antioxidant activity of the two extracts for both methods that were experimented. The n-BuOH extracts of the species possessed dose dependent scavenger activity evaluated after 30 min. of incubation with extracts, in all dose levels tested, whereas, the L. purpureum extract (1% concentration) exhibited the highest scavenging activity compared with Lamium album extract and reference substances. Five seconds after initiating the chemiluminiscence reaction measurements performed showed highest total antioxidant activity for Lamium album extract (1% concentration) compared with Lamium purpureum extract and reference substances. Differences in results between the two experienced methods may be due of reaction mechanisms, different reactivity - time-dependent reaction occurring - of compounds contained in these extracts. © 2013 University of Bucharest.


Grigore A.,National Institute for Chemical Pharmaceutical R and D ICCF Bucharest | Paraschiv I.,National Institute for Chemical Pharmaceutical R and D ICCF Bucharest | Colceru-Mihul S.,National Institute for Chemical Pharmaceutical R and D ICCF Bucharest | Bubueanu C.,National Institute for Chemical Pharmaceutical R and D ICCF Bucharest | And 2 more authors.
Romanian Biotechnological Letters | Year: 2010

In this paper two extraction methods of the Thymus vulgaris L. volatile oil have been comparatively investigated - steam distillation and extraction with non-polar solvents - reflected in oil quality and in the pharmacological activity. The qualitative analysis was performed by high performance thin layer chromatography (HPTLC) and the quantitative analysis by gas chromatography (GC). The antioxidant potential was determined by phosphomolybdenum reduction assay and DPPH assay. Results show that the Thymus vulgaris used for the present study belongs to thymol chemotype. Volatile oil obtained by steam distillation contains high amounts of thymol and p-cymene. For the sample obtained by non-polar solvent extraction, the above mentioned terpenes are the only volatile compounds detected by GC. Both samples exhibit antioxidant activity, slightly higher for volatile oil obtained by steam distillation. The study proves that the scavenging activity is not entirely due to volatile compounds but also to other liposoluble substances. © 2010 University of Bucharest.


Armatu A.,National Institute for Chemical Pharmaceutical R and D ICCF Bucharest | Colceru-Mihul S.,National Institute for Chemical Pharmaceutical R and D ICCF Bucharest | Bubueanu C.,National Institute for Chemical Pharmaceutical R and D ICCF Bucharest | Draghici E.,National Institute for Chemical Pharmaceutical R and D ICCF Bucharest | Pirvu L.,National Institute for Chemical Pharmaceutical R and D ICCF Bucharest
Romanian Biotechnological Letters | Year: 2010

Antioxidative effects of crude extracts from some Romanian Lamiaceae species have been studied with the use of three in vitro assays - DPPH reduction spectrophotometric assay, phosphomolybdenum method and chemiluminescence assay. Also, HPTLC fingerprints and total phenolic content of the extracts were analyzed in order to establish a relationship between chemical composition and pharmacological activity. All the assays confirmed the good antioxidant potential of the Lamiaceae species and in particular of the Nepetoideae subfamily. The most efficient activity was exhibited by Salvia officinalis L., Rosmarinus officinalis L. and Thymus vulgaris L. extracts, probably due to a high content of polyphenols. On the other hand, Lamioideae species like Lamium album L. and Leonurus cardiaca L. have a polyphenolic low content and, consequently, prove a weak or no antioxidant activity. The results were different depending on the applied test. Although Origanum vulgare L. has the highest content of polyphenols expressed as gallic acid, it showed a good scavenging activity only on DPPH free radical. © 2010 University of Bucharest.

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