National Engineering Research Center for Wheat

Zhengzhou, China

National Engineering Research Center for Wheat

Zhengzhou, China
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Feng Y.-L.,Henan Agricultural University | Wang K.-T.,Henan Agricultural University | Ma C.,Henan Agricultural University | Ma C.,Henan University of Science and Technology | And 4 more authors.
Biochemical and Biophysical Research Communications | Year: 2015

Abstract Vernalization requirement is an important characteristic in crop breeding. Wheat is a widely grown crop in the world that possesses enormous economic significance. To better understand the gene networks in vernalization process, we performed a high-throughput RNA sequencing analysis comparing the transcriptomes of spring and winter wheat cultivars, with and without vernalization (unpublished data). In this study, we selected six unigenes (CL14010, CL12788, CL176, Unigene 16777, CL8746 and Unigene10196) from our transcriptome analysis based on their expression differences to further characterize their function. Transient silencing of the six unigenes individually were achieved through virus-induced gene silencing (VIGS) using BSMV vector. The period from germination to spike differentiation were recorded and compared between plants underwent VIGS silencing and the control. Our result showed that VIGS of the six unigenes significantly shortened the period from seedling to double ridge (DR) stage. Resulting in SD period ranging from 59.8 ± 0.60 to 65.8 ± 0.48 days, compared to 85.0 ± 0.73 days in the control. The results indicated that these six unigenes function as suppressors in vernalization process and silence or down-regulation of these genes promoted flower development in wheat. Further characterization of these six unigenes and their function in vernalization and flowering control is needed. © 2015 Elsevier Inc. All rights reserved.


Lu H.,Henan Agricultural University | Wang C.,Henan Agricultural University | Wang C.,National Engineering Research Center for Wheat | Guo T.,Henan Agricultural University
Agronomy Journal | Year: 2013

Glutenin macropolymer (GMP), a major glutenin fraction, is affected by both genotypic and environmental factors. However, few studies have focused on the effects of heat stress on the characteristics of GMP in wheat (Triticum aestivum L.) grains. The highyielding winter wheat cultivar, Zhengmai 366, was grown in pots at the Scientific and Educational Station of Henan Agricultural University during the 2010-2011 and 2011-2012 growing seasons to investigate the response of GMP particle to high temperature (HT). The experiment was performed with a completely randomized design using three replicates. Plants were grown in the natural environment and then transferred to a climate-controlled greenhouse for two periods of HT treatments (38°C): (i) from 7 days after anthesis (DAA) to 10 DAA, and (ii) from 17 to 20 DAA, before being transferred back to the natural environment. Glutenin macropolymer particle size distribution was analyzed by laser diffraction. Results showed that heat stress led to a significant reduction in the proportion of GMP particles <10 μm, and a concomitant increase in particles >60 μm, yet had little effect on the number distribution of GMP particles. The proportion of GMP particles >60 μm was positively correlated with GMP and protein concentrations, but negatively correlated with protein yield. Heat stress significantly reduced the protein and starch yields despite the fact that the proportion of GMP particles >60 μm and the concentrations of GMP and protein all increased. © 2013 by the American Society of Agronomy, 5585 Guilford Road, Madison, WI 53711. All rights reserved.


Yan Y.-T.,National Engineering Research Center for Wheat | Li Y.-C.,National Engineering Research Center for Wheat | Meng F.-R.,Henan Agricultural University | Li J.-H.,National Engineering Research Center for Wheat | Yin J.,National Engineering Research Center for Wheat
Plant Physiology Communications | Year: 2010

Four spring cultivars with differently dominant vernalization genes were used to hybridize with a winter wheat cultivar 'Jing 841'. The F1 generation plants were identified by using PCR amplification specific to the dominant VRN1 genes, and some phenotypes of F1 generation plants were also analyzed. The results showed that the dominant allelic VRN1 genes were successfully introduced into the F1 plants and their seedling-heading periods were generally shorted due to the introduction of these dominant allelic sites. In three of four cross combinations, prominent differences of grains per spike were found between reciprocal crosses, which might be due to the cytoplasmic inheritance, and among them, the strong heterosis was observed in two crosses ('Xinchun 2' and 'Yumai 18' was used as the mother and father, respectively). In the four combinations, the character of thousand-grain weight all showed strong heterosis.


Lu H.F.,Henan Agricultural University | Wang C.Y.,Henan Agricultural University | Wang C.Y.,National Engineering Research Center for Wheat | Guo T.C.,Henan Agricultural University | And 2 more authors.
Shengtai Xuebao/ Acta Ecologica Sinica | Year: 2014

To explore the physiological mechanisms of stresses on nitrogen metabolism and protein accumulation in wheat grains, a pot experiment was performed at the Scientific & Educational Park of Henan Agricultural University from 2010 to 2012. Zhengmai 366 and Yunong 949, wheat cultivars with different gluten-strengths, were grown in 24-cm pots with 10 kg loam soil for the experiment, which was performed using a completely randomized design in three replicates. Plants were initially grown in the field environment and were then transferred to a climate-controlled greenhouse for the high temperature (HT) treatment. HT began from the 8th day after anthesis (DAA) and ended at the 11th DAA, with a high temperature of 38 °C for five hours from 11:00 to 16:00, after which the plants were returned to the field environment. The concentration of protein and its components, and the activities of glutamine synthetase (GS) and glutamic-pyruvic transaminase (GPT) in the grains were measured to evaluate the effects of the stresses. The results showed that HT, drought stress (DS) and their combination significantly increased protein and its components, concentrations, yet decreased the ratio of glutenin to gliadin. The protein accumulation was also reduced by stresses. For example, for Zhengmai 366, the protein yield significantly decreased by 20.7% under HT, by 16.2% under DS, and by 26.1% under a combination of HT and DS. In Yunong 949, the protein yield decreased by 12.4% under HT, by 11.9% under DS and by 15.8% under a combination of HT and DS. The grain weight significantly declined by 23.2% under HT, by 18.0% under DS and by 29.9% under a combination of HT and DS, in Zhengmai 366, and decreased by 24.0% under HT, by 16.0% under DS, and by 29.9% under a combination of HT and DS, in Yunong 949. However, the activities of GS and GPT were promoted under HT, DS and their combination. A correlation analysis showed that the activity of GS was positively correlated with the protein concentration in mature wheat grains at significant or extremely significant levels at 8, 17, 23, and 29 DAA. However, GS activity was negatively correlated with protein yield at extremely significant levels at 23 and 35 DAA, and it was negatively correlated with grain yield at significant or extremely significant levels at 8, 17, 23, 29 and 35 DAA. The activity of GPT was positively correlated with protein concentration at extremely significant levels at 11 and 17 DAA. However, GPT activity was negatively correlated with protein yield at extremely significant levels at 8, 17 and 23 DAA and negatively correlated with grain yield at significant or extremely significant levels at 8, 11, 17 and 23 DAA. The results also indicated that, under the present experimental conditions, the influence of the HT treatment was greater than that of the DS, and an overlap effect existed between HT and DS. In addition, Zhengmai 366, a high-gluten cultivar, showed more sensitivity to stresses than Yunong 949, a mid-gluten cultivar. These results may aid in understanding how HT and DS influence protein accumulation and may be useful in improving wheat quality.


Lu H.,Henan Agricultural University | Wang C.,Henan Agricultural University | Wang C.,National Engineering Research Center for Wheat | Guo T.,Henan Agricultural University | And 6 more authors.
Starch/Staerke | Year: 2014

High temperature (HT) and drought stress (DS) pose serious limitations to wheat (Triticum aestivum L.) grain development, which strongly influences grain yield and quality. In this study, we investigated changes in starch characteristics and starch granules of the high-yielding winter wheat cultivar Zhengmai 366 in response to HTand DS, both alone and in combination. HT, DS and the combination of these stressors led to a significant reduction in the proportion of A-type starch granules (>9.9 mm) to B-type starch granules (<9.9 mm), and the effects of HTand DS were significantly additive. The volume proportion of A-type starch granules (>9.9 mm) was positively correlated with the contents of AM, AP and starch as well as the grain yield (thousand grains weight) and was negatively correlated with the ratio of AM to AP, whereas there was a contrary correlation between the volume proportion of B-type starch granules (<9.9mm) and these values. HT, DS and HT plus DS led to a significant reduction in the content of AP and starch and grain yield, but these treatments had less of an impact on AM content. DS reduced the size of the small starch granules, while HTreduced the size of the large granules. The changes in morphology and size distribution of starch granules led to a reduction in starch content under both stress conditions, consequently reducing grain yield. HTplus DS had a much greater influence on starch characteristics and yield than HT or DS alone. © 2013 WILEY-VCH Verlag GmbH & Co.

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