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Ji L.,Beijing Forestry University | Wang Q.,Beijing Forestry University | Teixeira da Silva J.A.,Kagawa University | Yu X.N.,Beijing Forestry University | Yu X.N.,National Engineering Research Center for Floriculture
Scientia Horticulturae | Year: 2012

With rich germplasm resources, species of the genus Paeonia are exceptionally diverse in their flower color, flower type, plant height and so on. It is also well known that the genus has important ornamental and medicinal values. Hence, many researches especially Chinese take great efforts to study the genetic diversity. Even so, complex genetic backgrounds, vague genetic relationships and mixed cultivars still give rise to severe restrictions in breeding programmes. This review discusses recent literature on genetic diversity of Paeonia sect. Moutan DC and sect. Paeonia, which contain relatively rich germplasm resources from the level of comparative morphology, palynology, cytology, isozyme and molecular markers and DNA sequence analysis. Meantime, the advance research direction of genetic diversity in Paeonia is proposed. Comprehensively utilizing evidence in many levels and balanced development between tree peony and herbaceous peony are highlighted. © 2012 Elsevier B.V.

Shen M.,Beijing Forestry University | Wang Q.,Beijing Forestry University | Yu X.,Beijing Forestry University | Yu X.,National Engineering Research Center for Floriculture | Teixeira da Silva J.A.,Kagawa University
Scientia Horticulturae | Year: 2012

This review highlights the advances made on various aspects of herbaceous peony (Paeonia lactiflora Pall.) tissue culture, including the in vitro culture of underground buds, leaves and petioles, the induction of cluster buds, and callus induction. Tissue culture has the ability of speeding up the rate of propagation, reducing breeding time and meeting the needs of mass production. Problems that are currently being faced in herbaceous peony tissue culture are highlighted and possible viable solutions are provided. Since the vast majority of peony literature is published in Chinese, this review provides a unique and valuable resource and spring-board from which to initiate or further studies related to the in vitro culture of herbaceous peony. © 2012 Elsevier B.V.

Hao L.,Beijing Forestry University | Ma H.,Beijing Forestry University | Teixeira da Silva J.A.,P.O. Box 7 | Yu X.,Beijing Forestry University | Yu X.,National Engineering Research Center for Floriculture
Journal of the American Society for Horticultural Science | Year: 2016

The pollen morphology of 26 cultivars of herbaceous peony (Paeonia L.) was examined by scanning electron microscopy (SEM). Pollen grains of the cultivars were mostly subprolate to perprolate, tricolporate, or ellipsoidal in equatorial view, but rounded-trifid in polar view. The poles of pollen grains were mainly flat when viewed in cross-section and only a few appeared to have a circular or arc shape. In addition, the surface of the pollen grains was psilate, and the muri were partly or fully protuberant with a reticulate surface sculpture. However, the shapes and sizes of pollen grains as well as surface ornamentation varied significantly among cultivars with different chromosome numbers. The pollen grains from diploid cultivars were regular and full, and the reticulation holes were evenly distributed, while most of the pollen grains from triploid cultivars were empty flat shells with some heteromorphic pollen whose surface ornamentation was mostly rugulate-reticulate exine. The pollen grains from tetraploid cultivars were the largest although pollen morphology and surface ornamentation differed between Athena and Cream Delight. Six indices [polar axis length (P), equator axis length (E), P/E, pollen perforation diameter (D), ridge width (W), and D/W] were used in Q cluster analysis, which divided the 26 cultivars into three groups. Group I included eight diploid, four triploid, and one tetraploid cultivar. Group II included 12 triploid cultivars with complex genetic backgrounds. Group III included only one tetraploid cultivar Cream Delight. This work may provide an important palynological basis for studying taxonomy and hybrid breeding of herbaceous peony cultivars. © 2016, American Society for Horticultural Science. All rights reserved.

Wu H.,Beijing Forestry University | Yu X.,Beijing Forestry University | Yu X.,National Engineering Research Center for Floriculture | Da Silvac J.A.T.,Kagawa University | Lu G.,Beijing Forestry University
New Zealand Journal of Crop and Horticultural Science | Year: 2011

The underground buds of herbaceous peony (Paeonia lactiflora Pall.) 'Zhong Sheng Fen' were used to investigate the effects of indole-3-acetic acid (IAA) on culture initiation, benzyladenine (BA) on axillary shoot induction, and three auxins (IAA, indole-3-butyric acid [IBA] and 1-naphthyleneacetic acid [NAA]) on axillary shoot proliferation. In addition, methods to rejuvenate hyperhydric microshoots were established. Our results showed that the best initiation medium for 'Zhong Sheng Fen' was half-strength Murashige and Skoog (MS) medium supplemented withdouble the concentration of Ca 2+ (880 mg L -1), 1 mg L -1) BA, 0.5 mg L -1) gibberellic acid (GA 3) and 0.1 mg L -1) IAA. Axillary shoots were successfully induced by 2.0 mg L -1) BA. Several methods allowed the successful rejuvenation of hyperhydric microshoots, allowing them to develop normally. These included the addition of 3 g L -1) activated charcoal, the removal of ammonium nitrate from the medium, doubling the concentration of Ca 2+ or eliminating BA from the medium. © 2011 The Royal Society of New Zealand.

Wang Q.,Beijing Forestry University | Wang Q.,Tianjin Chengjian University | Cheng T.,National Engineering Research Center for Floriculture | Yu X.,Beijing Forestry University | And 3 more authors.
South African Journal of Botany | Year: 2014

Although herbaceous peony cultivars have been introduced and promoted widely, a key limitation to their use is their level of cold hardiness. In this study, the physiological and biochemical responses of six herbaceous peony cultivars to different temperatures and periods of exposure to freezing temperatures were assessed by analyzing changes in six indices. Under our experimental conditions, relative electric conductivity, soluble sugar concentration, soluble protein concentration, and malondialdehyde concentration generally increased as temperature declined, while proline content decreased. Superoxide dismutase activity fluctuated. After the same indicators were used to assess cold resistance over time at - 20 °C, the six cultivars could be ranked, in decreasing levels of cold resistance, as: 'Da Fu Gui', 'Fen Yu Nu', 'Kansas', 'Monsieur Jules Elie', 'Taff', and 'Pink Hawaiian Coral'. © 2014 South African Association of Botanists.

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