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Zhou B.,National Engineering Laboratory of Rice and By products Processing | Zhou B.,Hunan Province Key Laboratory of Grain and Oil Processing and Quality Control | Zhou B.,Central South University of forestry and Technology | Kang J.,National Engineering Laboratory of Rice and By products Processing | And 2 more authors.
Advanced Materials Research | Year: 2012

According to the results of the single temperature on Monascus anka mutant growth and monascus yellow pigments production in this paper, two stages in the fermentation temperature control strategy has been operated as following: the temperature was 34°C to improve the Monascus growth before culture 48 hour and then 32 was carried out to improve the yellow pigments production. The results demonstrated that two stages temperature control at 96 hours, the yield of monascus yellow pigments can arrive 164.51 OD units, it was 180.35%, 2165.48%, 107.66%, 5.76%, 33.23%, 36.74% higher than that of 26°C, 28°C, 30°C, 32°C, 34°C, 36°C fermentation, respectively. The maximum specific growth rate(μ) and the maximum specific production rate of yellow pigments(q Y) could arrive 0.0528 h -1 and 0.404 OD.g -1.h -1, and it was 5.30% and 3.22% higher than that of 34°C and 32°C, respectively. The specific production rate of yellow pigments was varied from 0.2 OD.g -1.h -1 to 0.4 OD.g -1.h -1 in whole fermentation. Two stage temperature control strategy could improve the yellow pigments production, increase μ and q Y and short the culture time. © (2012) Trans Tech Publications, Switzerland. Source

Ren J.,Central South University of forestry and Technology | Ren J.,National Engineering Laboratory of Rice and By products Processing | Ren J.,Hunan University | Ma L.,Central South University of forestry and Technology | And 7 more authors.
Biosensors and Bioelectronics | Year: 2013

Emergence of drug-resistance tuberculosis (DR-TB) represents a serious threat to the world. Most countries, especially the developing countries, lack efficacious and inexpensive tools for early detection of Mycobacterium tuberculosis (M. tuberculosis) resistance to antituberculosis drugs and for preventing the carriers from acquiring further resistance. This report describes an indirect series piezoelectric (ISP) system for simultaneous and early detection of M. tuberculosis resistance to antituberculosis drugs. The ISP system can continually monitor the frequency shift change profiles of 32 detection cells resulting from the changes of volatile metabolites group during M. tuberculosis growth. The quantification detection time (QDT) obtained from the frequency shift change profile was found to be characterisitc of M. tuberculosis resistance. A drug-free chamber was used as a control, in which 1% of M. tuberculosis isolate was added. By comparing the QDT obtained from growth of M. tuberculosis isolate in each drug-containing chamber with that in a drug-free chamber, forty strains of clinical M. tuberculosis isolates were categorized by our method. Comparison was also made using the agar proportion method (APM) and the BACTEC™ MGIT™ 960 system. The mean time for interpretive category of M. tuberculosis resistance measured by the ISP system is (111.7±28.45). h, which is more rapid than by the APM [(600.0±50.30). h] and the BACTEC™ MGIT™ 960 system [(140.3±39.52). h]. The present system is accurate, rapid and cost-effective with a wide application in early detection of DR-TB in the developing countries. © 2012 Elsevier B.V. Source

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