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Lian L.,Fujian Academy of Agricultural science | Lian L.,Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China | Lian L.,South China Base of National Key Laboratory of Hybrid Rice of China | Lian L.,National Engineering Laboratory of Rice | And 25 more authors.
Molecular Biology Reports | Year: 2014

Phosphoenolpyruvate carboxylase (PEPC) is known to play a key role in the initial fixation of CO2 in C4 photosynthesis. The PEPC gene from sugarcane (a C4 plant) was introduced into indica rice (Hang2), a process mediated by Agrobacterium tumefaciens. Integration patterns and copy numbers of the gene was confirmed by DNA blot analysis. RT-PCR and western blotting results showed that the PEPC gene was expressed at both the mRNA and protein levels in the transgenic lines. Real-time PCR results indicated that expression of the sugarcane PEPC gene occurred mostly in green tissues and changed under high temperature and drought stress. All transgenic lines showed higher PEPC enzyme activities compared to the untransformed controls, with the highest activity (11.1 times higher than the controls) being observed in the transgenic line, T34. The transgenic lines also exhibited higher photosynthetic rates. The highest photosynthetic rate was observed in the transgenic line, T54 (22.3 μmol m-2 s-1; 24.6 % higher than that in non-transgenic plants) under high-temperature conditions. Furthermore, the filled grain and total grain numbers for transgenic lines were higher than those for non-transgenic plants, but the grain filling (%) and 1,000-grain weights of all transgenic lines remained unchanged. We concluded that over-expression of the PEPC gene from sugarcane in indica rice (Hang2) resulted in higher PEPC enzyme activities and higher photosynthesis rates under high-temperature conditions. © 2014 The Author(s). Source


Huang F.,Fujian Academy of Agricultural science | Huang F.,Incubator of National Key Laboratory of Crop Germplasm Enhancement and Molecular Breeding Between Fujian and the Ministry of science and Technology Key Laboratory of Hybrid Rice Germplasm Enhancement and Molecular Breeding of South China | Huang F.,South China Base of National Key Laboratory of Hybrid Rice of China | Huang F.,National Engineering Laboratory of Rice | And 25 more authors.
Molecular Breeding | Year: 2014

Rice blast, caused by the fungus Magnaporthe oryzae, is one of the diseases most responsible for significantly decreasing the yield and quality of commercially grown rice. This study investigated the blast resistance response of japonica rice variety Yunyin (YY) treated with the Sichuan-43 isolate of M. oryzae. Seedlings of YY exhibited stronger resistance to blast than those of Lijiangxintuanheigu (LTH). The gene expression profile of YY treated with Sichuan-43 was determined using whole-genome microarray technology. Bioinformatics was used to identify putative resistance-related genes from the large number of genes assayed. Five candidate genes were further characterized by gene ontology classification analysis and pathway enrichment analysis, and were then integrated into various types of gene network regulation diagrams. Systematic bioinformatic analysis of the microarray provided a transcriptome map of YY, which helped to elucidate the mechanisms involved in blast resistance. Our results enhance the current understanding of the effects of rice blast resistance genes at the transcription level, and will facilitate further exploration of the molecular mechanism of blast resistance in YY. © 2014, Springer Science+Business Media Dordrecht. Source


Xu H.,Fujian Academy of Agricultural science | Xu H.,Incubator of National Key Laboratory of Fujian Crop Germplasm Innovation | Xu H.,Key Laboratory of Germplasm Innovation | Xu H.,South China Base of National Key Laboratory of Hybrid Rice of China | And 39 more authors.
Plant Biotechnology Journal | Year: 2015

Summary: Lipid peroxidation plays a major role in seed longevity and viability. In rice grains, lipid peroxidation is catalyzed by the enzyme lipoxygenase 3 (LOX3). Previous reports showed that grain from the rice variety DawDam in which the LOX3 gene was deleted had less stale flavour after grain storage than normal rice. The molecular mechanism by which LOX3 expression is regulated during endosperm development remains unclear. In this study, we expressed a LOX3 antisense construct in transgenic rice (Oryza sativa L.) plants to down-regulate LOX3 expression in rice endosperm. The transgenic plants exhibited a marked decrease in LOX mRNA levels, normal phenotypes and a normal life cycle. We showed that LOX3 activity and its ability to produce 9-hydroperoxyoctadecadienoic acid (9-HPOD) from linoleic acid were significantly lower in transgenic seeds than in wild-type seeds by measuring the ultraviolet absorption of 9-HPOD at 234 nm and by high-performance liquid chromatography. The suppression of LOX3 expression in rice endosperm increased grain storability. The germination rate of TS-91 (antisense LOX3 transgenic line) was much higher than the WT (29% higher after artificial ageing for 21 days, and 40% higher after natural ageing for 12 months). To our knowledge, this is the first report to demonstrate that decreased LOX3 expression can preserve rice grain quality during storage with no impact on grain yield, suggesting potential applications in agricultural production. © 2014 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd. Source


Luo X.,Fujian Academy of Agricultural science Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China | Luo X.,Incubator of National Key Laboratory of Fujian Crop Germplasm Innovation and Molecular Breeding between Fujian and Ministry of science and Technology | Luo X.,South China Base of National Key Laboratory of Hybrid Rice of China | Luo X.,National Engineering Laboratory of Rice | And 20 more authors.
Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis | Year: 2016

A new method based on near-infrared reflectance spectroscopy (NIRS) analysis was explored to determine the content of rice-resistant starch instead of common chemical method which took long time was high-cost. First of all, we collected 62 spectral data which have big differences in terms of resistant starch content of rice, and then the spectral data and detected chemical values are imported chemometrics software. After that a near-infrared spectroscopy calibration model for rice-resistant starch content was constructed with partial least squares (PLS) method. Results are as follows: In respect of internal cross validation, the coefficient of determination (R2) of untreated, pretreatment with MSC+1thD,pretreatment with 1thD+SNV were 0.920 2,0.967 0 and 0.976 7 respectively. Root mean square error of prediction(RMSEP)were 1.533 7,1.011 2 and 0.837 1 respectively. In respect of external validation, the coefficient of determination (R2) of untreated, pretreatment with MSC+1thD, pretreatment with 1thD+SNV were 0.805, 0.976 and 0.992 respectively. The average absolute error was 1.456, 0.818, 0.515 respectively. There was no significant difference between chemical and predicted values (Turkey multiple comparison), so we think near infrared spectrum analysis is more feasible than chemical measurement. Among the different pretreatment, the first derivation and standard normal variate (1thD+SNV) have higher coefficient of determination (R2) and lower error value whether in internal validation and external validation. In other words, the calibration model has higher precision and less error by pretreatment with 1thD+SNV. © 2016, Peking University Press. All right reserved. Source

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