National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines

Beijing, China

National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines

Beijing, China
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Wang S.,Chinese Institute of Materia Medica | Wang S.,Shaanxi University of Chinese Medicine | Hao L.-J.,Chinese Institute of Materia Medica | Zhu J.-J.,Chinese Institute of Materia Medica | And 7 more authors.
Phytomedicine | Year: 2014

The traditional after-harvesting drying method of C. morifolium cv. Hang-ju (HJ) is sun drying, but recently sulfur fumigation is increasingly used as a cheap and convenient method. However, the effects of sulfur fumigation on chemical constituents and potential activities of HJ were unknown. A comprehensively comparison of the chemical profiles between non-fumigated HJ (NHJ) and sulfur-fumigated HJ (SHJ) was conducted by HPLC fingerprints analysis and the discrepant peaks were identified or tentatively assigned by HPLC-ESI/MSn. Dramatic chemical changes were found that the contents of 4 flavonoid aglycones remarkably increased while those of 7 glycosides significantly reduced which suggested that sulfur-fumigation induced flavonoid glycosides transformed into aglycons by hydrolysis reaction. A significant loss of hydroxycinnamoylquinic acids showed the sulfur fumigation was a destructive effect on HJ. Principal component analysis (PCA) was employed to rapidly discriminate NHJ and SHJ samples. By ICP-OES analysis, it was found that the residue of sulfur of SHJ were three times higher than NHJ (p < 0.05). The antioxidant activity of NHJ and SHJ were evaluated by DPPH and FRAP assay, and the results showed that NHJ had much stronger antioxidant activities than SCF (p < 0.05). Combining the results of chemical analysis, residue of sulfur and pharmacological evaluation, it showed that the sulfur fumigation was a destructive effect on HJ. © 2013 Elsevier GmbH.


Gao H.,Chinese Institute of Materia Medica | Gao H.,National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines | Wang Z.,Chinese Institute of Materia Medica | Wang Z.,National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines | And 2 more authors.
Frontiers of Medicine in China | Year: 2011

Traditional Chinese medicine (TCM) has been widely used for the prevention and treatment of various diseases for a long time in China. Due to its proven efficacy, wide applications, and low side effect, TCM has increasingly attracted worldwide attention. However, one of the biggest challenges facing the clinical practice of TCM is the uncontrollable quality. In this review, the progress of the development and the current status of quality standard as well as new quality control techniques introduced in Chinese Pharmacopoeia (2010 edition), such as liquid chromatography hyphenated mass spectrometry (LC-MS), fingerprint, quantitative analysis of multicomponents by single-marker (QAMS), thin layer chromatography bio-autographic assay (TLC-BAA), and DNA molecular marker technique, are briefly overviewed. © Higher Education Press and Springer-Verlag Berlin Heidelberg 2011.


Li Y.-R.,Chinese Institute of Materia Medica | Li Y.-R.,National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines | Xu L.,Chinese Institute of Materia Medica | Li C.,Chinese Institute of Materia Medica | And 5 more authors.
Journal of Asian Natural Products Research | Year: 2014

A phytochemical investigation on the whole plant of Aconitum tanguticum (Ranunculaceae) resulted in the isolation and characterization of two new phenylpropanoid glycosides (1 and 2). Their structures were elucidated as 4-hydroxyphenethoxy-8-O-β-d-[6-O-(4-O-β-d-glucopyranosyl)-sinapoyl] -glucopyranoside (1) and 3,4-dimethoxy-trans-cinnamic acid-9-O-β-d- allopyranoside (2) on the basis of spectroscopic data (1D NMR, 2D NMR, and MS) and comparison with the literature data. © 2014 © 2014 Taylor & Francis.


Xu L.,Chinese Institute of Materia Medica | Xu L.,National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines | Xu L.,Jiangxi University of Traditional Chinese Medicine | Luo M.,Chinese Institute of Materia Medica | And 7 more authors.
Journal of Asian Natural Products Research | Year: 2013

Three new phenolic glycosides, such as (Z)-sinapic acid-4-O-β-d- allopyranoside (1), 3,4-dihydroxyphenethoxy-8-O-β-d-[6-O-(4-O-β-d- glucopyranosyl)-feruloyl]-glucopyranoside (2), and 4-dihydroxyphenethoxy-8-O- β-d-[6-O-(4-O-β-d-glucopyranosyl)-feruloyl]-glucopyranoside (3) were isolated from the EtOH extract of whole plant of Aconitum tanguticum (Maxim.) Stapf. The structures of the new compounds were elucidated by spectroscopic methods, and the total 1H NMR and 13C NMR chemical shifts were assigned. © 2013 Copyright Taylor and Francis Group, LLC.


Xu L.,Chinese Institute of Materia Medica | Xu L.,National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines | Xu L.,Jiangxi University of Traditional Chinese Medicine | Zhang X.,Hunan University | And 6 more authors.
Journal of Asian Natural Products Research | Year: 2013

Two new flavonol glycosides characterized as quercetin 3-O-α-l- rhamnopyranosyl-(1 → 2)-[β-d-glucopyranosyl-(1 → 3)-α-l-(4-O-trans-p-coumaroylrhamnopyranosyl)-(1 → 6)]-β-d-galactopyranoside-7-O-α-l-rhamnopyranoside (1) and kaempferol 3-O-α-l-rhamnopyranosyl-(1 → 2)-[β-d-glucopyranosyl-(1 → 3)-α-l-(4-O-trans-p-coumaroyl rhamnopyranosyl)-(1 → 6)]-β-d-galactopyranoside-7-O-α-l-rhamnopyranoside (2), together with two known flavonol glycosides quercetin 3-O-α-l-rhamnopyranosyl-(1 → 2)- [α-l-rhamnopyranosyl-(1 → 6)]-β-d-galactopyranoside-7-O- α-l-rhamnopyranoside (3) and kaempferol 3-O-α-l-rhamnopyranosyl-(1 → 2)-[α-l-rhamnopyranosyl-(1 → 6)]-β-d-galactopyranoside-7- O-α-l-rhamnopyranoside (4), were isolated from the whole plant of Aconitum tanguticum (Maxim.) Stapf. The structures of the new compounds were elucidated by spectroscopic methods, and the total 1H and 13C NMR chemical shifts were assigned. © 2013 Copyright Taylor and Francis Group, LLC.


Xu L.,Chinese Institute of Materia Medica | Li Y.-R.,Chinese Institute of Materia Medica | Li C.,Chinese Institute of Materia Medica | Li C.,National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines | And 4 more authors.
Zhongguo Zhongyao Zazhi | Year: 2013

Nineteen compounds were isolated from the whole plants of Aconitum tanguticum by means of various of chromatographic techniques such as silica gel, ODS, sephadex LH-20 and preparative HPLC, and their structures were elucidated as syringin(1), vanillic acid-4-O-β-D-allopyranoside(2), (E)-ferulic acid 4-O-β-D-allopyranoside(3), (E)-ferulic acid-4-O-β-glucopysoside(4), (E)-sinapic acid-4-O-β-glucopyranoside(5), (E)-4-hydroxycinnamyl alcohol 4-O-β-D-glucopyranoside(6), quercetin 3-O-α-L-rhamnopyranosyl-(1→2)- [α-L-rhamnopyranosyl-(1→6)]- β-D-galactopyranoside-7-O-α-L-rhamnopyranoside(7), kaempferol 3-O-α-L-rhamnopyranosyl-(1→2)- [α-L-rhamnopyranosyl-(1→6)]- β-D-galactopyranside-7-O-α-L-rhamnopyranoside(8), quercetin 3-O-α-L-rhamnopyranosyl-(1→6)- β-D-glucopyranoside-7-O-α-L-rhamnopyranoside(9), kaempferol 3-O-[β-D-glucopyranosyl-(1→3)- (4-O-trans-p-coumaroyl)]-α-L-rhamnopyranosyl- (1→6)-β-D-galactopyranside-7-O-α- L-rhamnopyranoside(10), quercetin 3-O-[β-D-glucopyranosyl- (1→3)-(4-O-trans-p-coumaroyl)]- α-L-rhamnopyranosyl-(1→6)-β- D-galactopyranoside-7-O-α-L-rhamnopyranoside(11), salidroside(12), 2-(3, 4-dihydroxyphenyl)ethanol 1-O-β-D-glucopyranoside(13), (7S, 8R)-dehydrodiconiferyl alcohol-9'-O-β-D-glucopyranoside(14), citrusin B(15), heteratisine(16), tanaconitine(17), shanzhiside methyl ester(18)and icariside B1(19). Except compounds 4, 13, 16 and 17, the other compounds were separated from the species for the first time.


Yin G.-P.,Chinese Institute of Materia Medica | Yin G.-P.,National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines | Li L.-C.,Southwest University of Science and Technology | Zhang Q.-Z.,Chinese Institute of Materia Medica | And 9 more authors.
Journal of Natural Products | Year: 2014

Eleven new sesquiterpenoids, wenyujinins A-K (1-11), and a new monoterpenoid, wenyujinin L (12), were isolated from the rhizomes of Curcuma wenyujin. Their structures and relative configurations were elucidated using 1D and 2D NMR, X-ray crystallographic analysis, and HRESIMS data. The absolute configurations of 1, 2, 3, 4, 6, 8, 9, and 10 were determined by comparison of the experimental and calculated ECD spectra. The absolute configuration of 5 was determined from the ECD data of the [Rh2(OCOCF3)4] complex, whereas those of 7 and 12 were determined from the ECD spectra of the compounds alone. Compounds 7 and 7a strongly inhibited the induction of NO production by LPS, with IC50 values of 7.6 and 8.5 μM, respectively. Compounds 6 and 10 moderately inhibited NO production with IC50 values of 47.7 and 48.6 μM, respectively. © 2014 The American Chemical Society and American Society of Pharmacognosy.


Gao H.,Chinese Institute of Materia Medica | Gao H.,National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines | Song Z.,Chinese Pharmacopoeia Commission | Wang Z.,Chinese Institute of Materia Medica | And 4 more authors.
Zhongguo Zhongyao Zazhi | Year: 2012

Based on the previous literatures, the overview on the history and recent advance of the quantitative analysis of multi-components by single-marker (QAMS) was summarized. The key questions of QAMS were also highlighted. It could be considered as a feasible method for the quality control of Traditional Chinese Medicines.


Li C.,Chinese Institute of Materia Medica | Li C.,National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines | Lin L.,Hunan University | Luo M.,Chinese Institute of Materia Medica | And 5 more authors.
Zhongguo Zhongyao Zazhi | Year: 2011

Objective: To study the chemical constituents in the fruits of Siraitia grosvenorii. Method: Isolation and purification of the constituents were carried out on column chromatography. Their structures were identified by NMR and MS spectral analysis. Result: Six compounds were isolated and elucidated as mogroside IIIA1 (1), siamenoside I (2), mogroside IVa (3), mogroside IVe (4), mogroside V (5) and 11-oxo-mogroside V(6), respectively. Conclusion: Compound 1, mogrol-24-O-β-D-glucopyranosyl(1→2)-[β-D- glucopyranosyl(1→6)]-β-D-glucopyranoside, was identified as a new natural product from the fruits of S. grosvenorii.


PubMed | Hunan University, National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines and Chinese Institute of Materia Medica
Type: Journal Article | Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica | Year: 2014

Nineteen compounds were isolated from the whole plants of Aconitum tanguticum by means of various of chromatographic techniques such as silica gel, ODS, sephadex LH-20 and preparative HPLC, and their structures were elucidated as syringin (1), vanillic acid-4-O-beta-D-allopyranoside (2), (E) -ferulic acid 4-O-beta-D-allopyranoside (3), (E) -ferulic acid-4-O-beta-glucopysoside (4), (E) -sinapic acid-4-O-beta-glucopyranoside (5), (E) 4-hydroxycinnamyl alcohol 4-O-beta-D-glucopyranoside (6), quercetin 3-O-alpha-L-rhamnopyranosyl-(1 --> 2) -[alpha-L-rhamnopyranosyl-(1 --> 6)] -beta-D-galactopyranoside-7-O-alpha-L-rhamnopyranoside (7), kaempferol 3-O-alpha-L-rhamnopyranosyl-(1 --> 2) - [alpha-L-rhamnopyranosyl-(1 --> 6)] -beta-D-galactopyranside-7-O-alpha-L-rhamnopyranoside (8), quercetin 3-O-alpha-L-rhamnopyranosyl-(1 --> 6) -beta-D-glucopyranoside-7-O-alpha-L-rhamnopyranoside (9), kaempferol 3-O-[beta-D-glucopyranosyl-(1 --> 3)-(4-O-trans-p-coumaroyl) ] -alpha-L-rhamnopyranosyl-(1 --> 6) -beta-D-galactopyranside-7-O-alpha-L-rhamnopyranoside (10), quercetin 3-O- [beta-D-glucopyranosyl-(1 --> 3 ) -(4-O-trans-p-coumaroyl)] -alpha-L-rhamnopyranosyl-(1--> 6) -beta-D-galactopyranoside-7-O-alpha-L-rhamnopyranoside (11), salidroside (12), 2-(3,4-dihydroxyphenyl) ethanol 1-O-beta-D-glucopyranoside (13), (7S, 8R) -dehydrodiconiferyl alcohol-9-O-beta-D-glucopyranoside (14), citrusin B (15), heteratisine (16), tanaconitine (17), shanzhiside methyl ester (18) and icariside B1 (19). Except compounds 4, 13, 16 and 17, the other compounds were separated from the species for the first time.

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