Time filter

Source Type

Wang Z.-M.,Chinese Institute of Materia Medica | Wang Z.-M.,National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine
Zhongguo Zhongyao Zazhi | Year: 2013

Recently the safety of Heshouwu become a focus, but the reasons of its hepotoxicity are confused. On the basis of literature research, some findings on species and usage custom maybe supply some clues to explain the reasons of its hepotoxicity. Heshouwu had red Heshouwu (male) and white Heshouwu (female) in ancient literature, and traditional usage was use of the male and female together. The Latin name of red Heshouwu is Fallopia multiflora, and that of the white one is F.multiflora var. multiflora. Source

Chen L.,Tokyo University of Pharmacy and Life Science | Chen L.,Chinese Institute of Materia Medica | Chen L.,National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine | Kotani A.,Tokyo University of Pharmacy and Life Science | And 6 more authors.
Analytical Sciences | Year: 2015

We have proposed an assessment methods to estimate the measurement relative standard deviation (RSD) of chromatographic peaks in quantitative HPLC for herbal medicines by the methodology of ISO 11843 Part 7 (ISO 11843-7:2012), which provides detection limits stochastically. In quantitative HPLC with UV detection (HPLC-UV) of Scutellaria Radix for the determination of baicalin, the measurement RSD of baicalin by ISO 11843-7:2012 stochastically was within a 95% confidence interval of the statistically obtained RSD by repetitive measurements (n = 6). Thus, our findings show that it is applicable for estimating of the repeatability of HPLC-UV for determining baicalin without repeated measurements. In addition, the allowable limit of the "System repeatability" in "Liquid Chromatography" regulated in a pharmacopoeia can be obtained by the present assessment method. Moreover, the present assessment method was also successfully applied to estimate the measurement RSDs of quantitative three-channel liquid chromatography with electrochemical detection (LC-3ECD) of Chrysanthemi Flos for determining caffeoylquinic acids and flavonoids. By the present repeatability assessment method, reliable measurement RSD was obtained stochastically, and the experimental time was remarkably reduced. 2015 © The Japan Society for Analytical Chemistry. Source

Dai L.-P.,Henan University of Traditional Chinese Medicine | Dai L.-P.,Chinese Institute of Materia Medica | Dai L.-P.,Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment | Li C.,Chinese Institute of Materia Medica | And 9 more authors.
Molecules | Year: 2015

Three types of ent-kaurane diterpenoids were isolated from the aerial parts of Isodon excisoides, including three new diterpenoids, 1α,7α,14β-trihydroxy-20-acetoxy-entkaur- 15-one (1); 1α,7α,14β,18-tetrahydroxy-20-acetoxy-ent-kaur-15-one (2); and 1α-acetoxy- 14β-hydroxy-7α,20-epoxy-ent-kaur-16-en-15-one (3); together with six known diterpenes henryin (4); kamebanin (5); reniformin C (6); kamebacetal A (7); kamebacetal B (8); and oridonin (9). The structures of the isolated compounds were elucidated by means of nuclear magnetic resonance spectroscopy and high-resolution mass spectrometry in conjunction with published data for their analogs, as well as their fragmentation patterns. Compounds 5 and 9 were isolated from Isodon excisoides for the first time. To explore the structure-activity relationships of the isolated compounds, they were tested for their cytotoxic effects against five human cancer cell lines: HCT-116, HepG2, A2780, NCI-H1650, and BGC-823. Most of the isolated compounds showed certain cytotoxic activity against the five cancer cell lines with IC50 values ranging from 1.09-8.53 μM. Among the tested compounds, compound 4 exhibited the strongest cytotoxic activity in the tested cell lines, with IC50 values ranging from 1.31-2.07 μM. Compounds 1, 6, and 7 exhibited selective cytotoxic activity. © 2015 by the authors; licensee MDPI, Basel, Switzerland. Source

Dong H.,Jilin University | Ren J.-X.,Changchun University of Chinese Medicine | Wang J.-J.,Chinese Institute of Materia Medica | Wang J.-J.,National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine | And 8 more authors.
Evidence-based Complementary and Alternative Medicine | Year: 2016

Hirudo (Shuizhi in Chinese) is an important Chinese medicine, which possesses many therapeutic properties for the treatment of the cerebral hemorrhage and other thrombosis-related diseases. The phytochemical investigation gave more than 51 compounds including pteridines, phosphatidylcholines, glycosphingolipids, and sterols, as well as some bioactive peptides from the Shuizhi derived from three animal species recorded in the current Chinese Pharmacopoeia. The pharmacological studies on the Shuizhi have revealed various activities such as anticoagulation, antithrombosis, antiatherosclerosis, antiplatelet aggregation, antitumor and anti-inflammatory as well as hemorheology improvement, and protective effects against cerebral ischemia-reperfusion injury. However, some important issues based on the traditional uses of Shuizhi are still not clear. The aim of the present review is to provide comprehensive knowledge on the ethnopharmacology, phytochemistry, and pharmacological activities of Shuizhi. It will provide a potential guidance in exploring main active compounds of Shuizhi and interpreting the action mechanism for the further research. © 2016 Han Dong et al. Source

Ma Y.,Chinese Institute of Materia Medica | Gao H.,Chinese Institute of Materia Medica | Gao H.,National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine | Liu J.,China National Center for Biotechnology Development | And 6 more authors.
Journal of Liquid Chromatography and Related Technologies | Year: 2014

The qualitative and quantitative analyses by HPLC and LC-DAD-MS/MS were conducted for the quality consistency evaluation of Compound Kushen Injection (CKI). For fingerprint analysis, 8 peaks were selected as the characteristic peaks in the HPLC chromatograms of 27 different batches of CKI samples and their similarities were more than 0.90, except the four samples. For the typical HPLC profile, peak identification was carried out by HPLC and LC-MS/MS analyses and as a result, 21 chromatographic peaks were identified or characterized based on the comparison of retention time and UV spectra with authentic compounds as well as by summarized MS fragmentation rules. Six of these identified compounds, including oxymatrine, oxysophocarpine, N-methylcytisine, matrine, sophocarpine, and trifolirhizin, were simultaneously determined and the results showed that the total amounts of six marker compounds were variable, to some extent, which was deduced to be related to the quality of the crude materials and the manufacturing process employed. Supplemental materials are available for this article. Go to the publisher's online edition of the Journal of Liquid Chromatography & Related Technologies to view the supplemental file. © 2014 Copyright Taylor and Francis Group, LLC. Source

Discover hidden collaborations