National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine

Beijing, China

National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine

Beijing, China

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Liang J.,Chinese Institute of Materia Medica | Liang J.,National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine | Gao H.,Chinese Institute of Materia Medica | Gao H.,National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine | And 7 more authors.
Evidence-based Complementary and Alternative Medicine | Year: 2014

Chemical profiling is always the first task in the standardization and modernization of Traditional Chinese Medicine. HPLC and LC-MS were employed to find out the common chromatographic peaks in various batches of Tianshu Capsule (TSC) and the contribution of the characteristic peaks from individual herbs to the whole chromatographic profile of TSC sample. A total of 38 constituents were identified in TSC sample based on the comparison of retention time and UV spectra with authentic compounds as well as by summarized MS fragmentation rules and matching of empirical molecular formula with those of published components. This is the first systematic report on the chemical profiling of the commercial TSC product, which provides the sufficiently chemical evidence for the global quality evaluation of TSC products. © 2014 Juanjuan Liang et al.


Chen L.,Tokyo University of Pharmacy and Life Science | Chen L.,Chinese Institute of Materia Medica | Chen L.,National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine | Kotani A.,Tokyo University of Pharmacy and Life Science | And 6 more authors.
Analytical Sciences | Year: 2015

We have proposed an assessment methods to estimate the measurement relative standard deviation (RSD) of chromatographic peaks in quantitative HPLC for herbal medicines by the methodology of ISO 11843 Part 7 (ISO 11843-7:2012), which provides detection limits stochastically. In quantitative HPLC with UV detection (HPLC-UV) of Scutellaria Radix for the determination of baicalin, the measurement RSD of baicalin by ISO 11843-7:2012 stochastically was within a 95% confidence interval of the statistically obtained RSD by repetitive measurements (n = 6). Thus, our findings show that it is applicable for estimating of the repeatability of HPLC-UV for determining baicalin without repeated measurements. In addition, the allowable limit of the "System repeatability" in "Liquid Chromatography" regulated in a pharmacopoeia can be obtained by the present assessment method. Moreover, the present assessment method was also successfully applied to estimate the measurement RSDs of quantitative three-channel liquid chromatography with electrochemical detection (LC-3ECD) of Chrysanthemi Flos for determining caffeoylquinic acids and flavonoids. By the present repeatability assessment method, reliable measurement RSD was obtained stochastically, and the experimental time was remarkably reduced. 2015 © The Japan Society for Analytical Chemistry.


Chen L.,Tokyo University of Pharmacy and Life Science | Chen L.,Chinese Institute of Materia Medica | Chen L.,National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine | Kotani A.,Tokyo University of Pharmacy and Life Science | And 6 more authors.
Chemical and Pharmaceutical Bulletin | Year: 2015

For the determination of seven caffeoylquinic acids [neochlorogenic acid (NcA), cryptochlorogenic acid (CcA), chlorogenic acid (CA), caffeic acid (CfA), isochlorogenic acid A (Ic A), isochlorogenic acid B (Ic B), isochlorogenic acid C (Ic C)] and two flavonoids [luteolin 7-O-glucoside (LtG) and luteolin (Lt)], a three-channel liquid chromatography with electrochemical detection (LC-3ECD) method was established. Chromatographic peak heights were proportional to each concentration, ranging from 2.5 to 100 ng/mL for NcA, CA, CcA, and CfA, and ranging from 2.5 to 250 ng/mL for LtG, Ic B, Ic A, Ic C, and Lt, respectively. The present LC-3ECD method was applied to the quantitative analysis of caffeoylquinic acids and flavonoids in four cultivars of Chrysanthemum morifolium flowers and their sulfur-fumigated products. It was found that 60% of LtG and more than 47% of caffeoylquinic acids were lost during the sulfur fumigation processing. Sulfur fumigation showed a destructive effect on the C. morifolium flowers. In addition, principle component analyses (PCA) were performed using the results of the quantitative analysis of caffeoylquinic acids and flavonoids to compare the "sameness" and "differences" of these analytes in C. morifolium flowers and the sulfur-fumigated products. PCA score plots showed that the four cultivars of C. morifolium flowers were clearly classified into four groups, and that significant differences were also found between the non-fumigated C. morifolium flowers and the sulfur-fumigated products. Therefore, it was demonstrated that the present LC-3ECD method coupled with PCA is applicable to the variation analysis of different C. morifolium flower samples. © 2015 The Pharmaceutical Society of Japan.


Dong H.,Jilin University | Ren J.-X.,Changchun University of Chinese Medicine | Wang J.-J.,Chinese Institute of Materia Medica | Wang J.-J.,National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine | And 8 more authors.
Evidence-based Complementary and Alternative Medicine | Year: 2016

Hirudo (Shuizhi in Chinese) is an important Chinese medicine, which possesses many therapeutic properties for the treatment of the cerebral hemorrhage and other thrombosis-related diseases. The phytochemical investigation gave more than 51 compounds including pteridines, phosphatidylcholines, glycosphingolipids, and sterols, as well as some bioactive peptides from the Shuizhi derived from three animal species recorded in the current Chinese Pharmacopoeia. The pharmacological studies on the Shuizhi have revealed various activities such as anticoagulation, antithrombosis, antiatherosclerosis, antiplatelet aggregation, antitumor and anti-inflammatory as well as hemorheology improvement, and protective effects against cerebral ischemia-reperfusion injury. However, some important issues based on the traditional uses of Shuizhi are still not clear. The aim of the present review is to provide comprehensive knowledge on the ethnopharmacology, phytochemistry, and pharmacological activities of Shuizhi. It will provide a potential guidance in exploring main active compounds of Shuizhi and interpreting the action mechanism for the further research. © 2016 Han Dong et al.


Gao H.,Chinese Institute of Materia Medica | Gao H.,National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine | Wu X.,Chinese Institute of Materia Medica | Wu X.,National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine | And 6 more authors.
Zhongguo Zhongyao Zazhi | Year: 2011

Eight compounds were isolated from Bufonis periostracum by repeated column chromatography on silica gel, ODS and Sephadex LH-20 and their structures were characterized as palmitatic acid cholesteryl ester(1), cholesterol(2), 5α, 8α-epidioxycholesta-6-en-3β-ol(3) , cholest-5-en-3β, 7β-diol(4), cholest-7-en-3β, 5α, 6β-triol(5), 3-octaddecyloxy-1, 2-propanediol(6), isisamide(7) and bufothionine(8) on the base of spectral analysis. Compounds 1-8 were isolated from Bufonis periostracum for the first time and compounds 3,5,6,7 were obtained from Bufo bufo gargarizans and Bufo genus for the first time. The bioassays showed all tested samples displayed no antitumor activity against the cell lines such as A549, BeL 7402, HGC-27 and HL-60, except the control compound bufalin.


Chen L.,Tokyo University of Pharmacy and Life Science | Chen L.,Chinese Institute of Materia Medica | Chen L.,National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine | Hakamata H.,Tokyo University of Pharmacy and Life Science | And 6 more authors.
Journal of Pharmaceutical and Biomedical Analysis | Year: 2014

A novel three-channel isocratic elution high performance liquid chromatography with electrochemical detection (3LC-ECD) system was developed for the simultaneous determination of various bioactive redox components in Shuang-Huang-Lian (SHL) preparations. This 3LC-ECD system consists of three isocratic elution flow ways, two switching valves, four columns, and three electrochemical detectors. Through alternately rotating switching valves to change the elution flow way, six caffeoylquinic acids, four flavonoids, and one phenylethanoid glycoside compound were simultaneously determined within 70. min by the present 3LC-ECD method, in which three compounds of protocatechuic aldehyde, propyl gallate, and kaempferol were used as internal standards. The results demonstrated that the present 3LC-ECD method has achieved desired linearity (r>. 0.999), precision (relative standard deviation <2.5%), accuracy (recovery, 95.6-103.6%), and high sensitivity (limit of detection, 0.11-0.90. ng/mL). In addition, we successfully determined the contents of these 11 bioactive redox components in 14 batches of SHL oral liquid and 12 batches of SHL lyophilized powder for injection produced by different manufacturers in China. © 2014 Elsevier B.V.


Ma Y.,Chinese Institute of Materia Medica | Gao H.,Chinese Institute of Materia Medica | Gao H.,National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine | Liu J.,China National Center for Biotechnology Development | And 6 more authors.
Journal of Liquid Chromatography and Related Technologies | Year: 2014

The qualitative and quantitative analyses by HPLC and LC-DAD-MS/MS were conducted for the quality consistency evaluation of Compound Kushen Injection (CKI). For fingerprint analysis, 8 peaks were selected as the characteristic peaks in the HPLC chromatograms of 27 different batches of CKI samples and their similarities were more than 0.90, except the four samples. For the typical HPLC profile, peak identification was carried out by HPLC and LC-MS/MS analyses and as a result, 21 chromatographic peaks were identified or characterized based on the comparison of retention time and UV spectra with authentic compounds as well as by summarized MS fragmentation rules. Six of these identified compounds, including oxymatrine, oxysophocarpine, N-methylcytisine, matrine, sophocarpine, and trifolirhizin, were simultaneously determined and the results showed that the total amounts of six marker compounds were variable, to some extent, which was deduced to be related to the quality of the crude materials and the manufacturing process employed. Supplemental materials are available for this article. Go to the publisher's online edition of the Journal of Liquid Chromatography & Related Technologies to view the supplemental file. © 2014 Copyright Taylor and Francis Group, LLC.


Li Z.-Y.,Chinese Institute of Materia Medica | Li Z.-Y.,National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine | Gao H.-M.,Chinese Institute of Materia Medica | Gao H.-M.,National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine | And 7 more authors.
Helvetica Chimica Acta | Year: 2012

The new secoiridoid sulfonates 1-3 were isolated from the 50% EtOH/H 2O extract of the sulfiting-processed Lonicera japonica Flos (LJF) by semi-prep. HPLC, and their structures were identified on the basis of mass spectrometry and NMR spectroscopy. HPLC and LC-DAD-MS/MS analyses of the different samples of LJF obtained by various process techniques suggested that the sulfur fumigation led to the decrease of secologanic acid (4) and the formation of secologanic acid-derived sulfonate 1 and its derivatives 2, 2a, and 3 in the crude materials, which revealed that sulfur fumigation, the traditional process technique, could alter the phytochemical profiles of some Chinese herbal medicines. Copyright © 2012 Verlag Helvetica Chimica Acta AG, Zürich, Switzerland.


Zhang H.-F.,Chinese Institute of Materia Medica | Zhang H.-F.,Henan University of Traditional Chinese Medicine | Zhang H.-F.,National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine | Yan L.-H.,Chinese Institute of Materia Medica | And 5 more authors.
Zhongguo Zhongyao Zazhi | Year: 2013

Eleven compounds were isolated from the leaves of Epimedium pubescens by means of various chromatographic techniques such as silica gel, MCI, Sephadex LH-20 and preparative HPLC. Their structures were identified as anhydroicaritin(1), icarisideII(2), 2″-O-rhamonosyl-icarisideII(3), desmethylanhydroicaritin(4), baohuosaideII(5), epimedokoreanin B(6), acuminatin(7), tricin(8), kaempferol(9), daidzein(10) and 4-hydroxy ethyl benzoate(11) on the basis of physicochemical properties and spectroscopic data analysis. Among them, compound 11 was isolated from Epimedium species for the first time, and other compounds were obtained from this plant for the first time.


Wang Z.-M.,Chinese Institute of Materia Medica | Wang Z.-M.,National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine
Zhongguo Zhongyao Zazhi | Year: 2013

Recently the safety of Heshouwu become a focus, but the reasons of its hepotoxicity are confused. On the basis of literature research, some findings on species and usage custom maybe supply some clues to explain the reasons of its hepotoxicity. Heshouwu had red Heshouwu (male) and white Heshouwu (female) in ancient literature, and traditional usage was use of the male and female together. The Latin name of red Heshouwu is Fallopia multiflora, and that of the white one is F.multiflora var. multiflora.

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