Lin J.,Fuzhou University |
Lin J.,National Engineering Laboratory for Highly Efficiently Enzyme Expression |
Lan S.,Fuzhou University |
Yang J.,Fuzhou University |
And 5 more authors.
Journal of Chinese Institute of Food Science and Technology | Year: 2013
Objective: The strain screened from nature was identified and its laccase-producing conditions were optimized to increase laccase activity. Method: A strain HYB07 with high yield of laccase was obtained by screening with PDA plates containing guaiacol and Remazol brilliant blue R, respectively. Strain HYB07 was identified as Cerrena uni-color via 18s rDNA sequence analysis and morphology observation. Based on high nitrogen and low carbon medium (LM3), the carbon sources, nitrogen sources, inducer and initial pH were optimized. Furthermore, the fermentation conditions such as inoculum volume, temperature, shaking speed and liquid volume in flask were explored. The optimal laccase-producing conditions were achieved: glucose 2%, Ammonium tartrate 1.5%, ABTS 0.05 mmol/L, initial pH5.0, inoculum volume 8%, temperature 25°C, shaking speed 200 r/min, liquid volume in flask 60 mL/250 mL, fermentation period 6 d. Conclusion: Under the optimal conditions, the maximum laccase activity was 79733 U/L, 41.5% higher than fermented with LM3 medium. Moreover, laccase production was significantly affected by Cu2+, and laccase activity was decreased by 87.64% absence of Cu2+.