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Madison, WI, United States

Huang Y.,National Cooperative Drug Discovery Group | Huang S.-X.,National Cooperative Drug Discovery Group | Ju J.,National Cooperative Drug Discovery Group | Tang G.,National Cooperative Drug Discovery Group | And 3 more authors.
Organic Letters | Year: 2011

Leinamycin (LNM, 1) biosynthesis is proposed to involveβ-alkylation of the polyketide intermediate, catalyzed by LnmKLM. Inactivation of lnmK, lnmL, or lnmM afforded mutant strains that accumulated LNM K-1 (2), K-2 (3), K-3 (4), and isomers LNM K-1′ (5), K-2′ (6), and K-3′ (7) whose polyketide origin was established by feeding experiments with sodium [1- 13C]acetate. These findings confirm the indispensability of LnmKLM in 1 biosynthesis and suggest that β-alkylation proceeds on the growing polyketide intermediate while bound to the LNM polyketide synthase. © 2011 American Chemical Society. Source

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