National Commission for Biotechnology NCBT

Damascus, United States

National Commission for Biotechnology NCBT

Damascus, United States
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Alnaasan Y.,International Center for Agricultural Research in the Dry Areas | Kumari S.G.,International Center for Agricultural Research in the Dry Areas | Van Leur J.A.G.,Australian Department of Primary Industries and Fisheries | Haj Kassem A.A.,University of Aleppo | Azmeh F.,National Commission for Biotechnology NCBT
Phytopathologia Mediterranea | Year: 2013

Reverse transcription-polymerase chain reaction analysis with two primer sets of luteoviruses was used to characterize an isolate of Chickpea chlorotic stunt virus (CpCSV, genus Polerovirus, family Luteoviridae) (SC402-08) collected from Lattakia, Syria, during the 2007-2008 chickpea growing season. Sequence analysis revealed that the coat protein gene of the isolate shared nucleotide sequence identities ranging from 97 to 98% with the CpCSV isolates from Egypt, Morocco and Syria. The capsid protein was separated as a protein of approximately 20 kDa in sodium dodecyl sulphate polyacrylamide gel electrophoresis, and was visually detected by its reaction with CpCSV monoclonal antibody in Western blot. SC402-08 isolate of CpCSV was purified from faba bean-infected plants, and yielded 112-182 μg of purified virions kg-1 of infected tissue. The purified preparation was injected into a white rabbit, and an antiserum was obtained and used to detect CpCSV in infected tissues by tissue-blot immunoassay. The antiserum obtained was able to detect CpCSV by the immunoassay up to a dilution of 1:1,024,000. © Firenze University Press.

Bogaerts S.,Lupinelaan 25 | Sparreboom M.,Naturalis Biodiversity Center | Pasmans F.,Ghent University | Almasri A.,National Commission for Biotechnology NCBT | And 3 more authors.
Salamandra | Year: 2013

The distribution, ecology and conservation status of the Syrian urodeles Salamandra infraimmaculata and Ommatotriton vittatus are poorly known. We present the results of a field study, conducted in February 2009. Salamandra infraimmaculata was found at six localities, ranging from 228 to 960 m a.s.l., and co-occurred with O. vittatus at three localities. All localities were near small, clear streams or springs. Temperatures ranged from 9.4 to 16.4°C, pH 7.5-8.5, GH 3-18 and KH 3-18. The distribution model of S. infraimmaculata reveals that the distribution of this species is nearly entirely shaped by precipitation in the coldest quarter "92.9% contribution to the model". The rarity of suitable surface waters is probably the main reason for the supposed scarcity of this species in northwestern Syria. Tapping a water source for drinking water resulted in one case in extensive mortality of larvae. Ommatotriton vittatus was found at nine different localities, ranging from 172 to 960 m a.s.l. Habitat characteristics, water quality and morphological data were recorded. The average total length of adult O. vittatus was 116 mm (range 93-138 mm, n = 22) for males and 93 mm (range 86-108 mm, n = 34) for females. Mean weight was 6.7 g for males and 3.9 for females. The mean body condition index of females was comparable to that of males. Water temperatures ranged from 8.7 to 14.6°C, pH 7.5-8.5, GH 3-18 and KH 3-18. The distribution model of O. vittatus reveals that the distribution of this species is mainly shaped by precipitation, both during the winter and summer periods. The collection of large numbers of adult O. vittatus for fish bait was observed. © 2013 Deutsche Gesellschaft für Herpetologie und Terrarienkunde e.V. (DGHT).

Alburaki M.,French National Center for Scientific Research | Alburaki M.,National Commission for Biotechnology NCBT | Moulin S.,French National Center for Scientific Research | Legout H.,French National Center for Scientific Research | And 3 more authors.
Apidologie | Year: 2011

Honeybees (Apis mellifera) from Syria (n=1,624), Lebanon (n=177), and Iraq (n=35) were collected for genetic analysis. These samples were analyzed by the cleaved amplified polymorphisms technique using mitochondrial DNA (mtDNA) as a molecular marker in their COI-COII intergenic region (Cytochrome Oxidase I and II). Polymerase chain reaction amplicons (500-950 bp) were subjected to restriction analysis by DraI enzyme. The restriction profiles were analyzed and compared with the existing mtDNA markers on polyacrylamide gels. Sixteen new haplotypes were identified, 14 of them on the African lineage A, one new haplotype on the North Mediterranean lineage C and one other on the West Mediterranean lineage M. DNA sequences of all these haplotypes were subsequently determined. We also identified five other haplotypes that had been described in a previous study. These five haplotypes were renamed and grouped more appropriately with the subgroup Z, rather than with the Oriental lineage O. The sequence structures of these novel haplotypes clearly display the specific characteristics of the African branch (A). However, they differ from the African haplotypes by a new restriction site; therefore, they have been moved into a new subgroup. We conclude that A. mellifera syriaca subspecies does not belong to the Oriental lineage O, but is situated geographically in an area of contact between the A and O lineages. © INRA, DIB-AGIB and Springer Science+Business Media B.V., 2011.

Monawekh R.,National Commission for Biotechnology NCBT | Azzam H.,Damascus University | Abbas S.,General Commission for Scientific Agricultural Researches | Murshed R.,Damascus University
International Journal of ChemTech Research | Year: 2014

The present study was carried out to evaluate the genetic diversity in barley (Hordeumvulgare L.) using SSR markers. Thirty two simple sequence repeats (SSRs) markers were used to characterize fifty five barley genotypes. Five markers showed monomorphic band profiles, while 27 discriminatory markers, generated clear patterns with high polymorphism markers produced a total of 203 alleles. The number of alleles per marker ranged from 2 to 36 with a mean of 7.52 alleles per locus. Eleven unique alleles were observed in nine genotypes. The Polymorphic information content (PIC) values ranged from 0.28 to 0.96 with a mean value of 0.65, and the gene diversity ranged from 0.31 to 0.96 with a mean value of 0.70.Our results showed that the gene diversity values were very similar to the PIC values and the number of alleles detected by SSR marker correlated positively with the genetic diversity value (r= 0.67**). The dendrogram discriminated all the genotypes and clustered them separately in two major groups according to their pedigree, and showed high levels of polymorphism among the genotypes included in this study.

Murshed R.,Damascus University | Najla S.,Damascus University | Albiski F.,National Commission for Biotechnology NCBT | Kassem I.,National Commission for Biotechnology NCBT | And 2 more authors.
Journal of Agricultural Science and Technology | Year: 2015

Salt stress negatively impacts crops yield throughout the world. Nine varieties of potato (Solanum tuberosum L.) were screened for salt stress tolerance by measuring in-vitro growth of the aerial plant parts, as well as roots. Salt stress was evaluated by adding 25, 50, 75, 100, 125, 150 and 200 mM of NaCl to Murashige- Skoog (MS) medium and compared to MS medium without NaCl. Plant length and stem thickness, leaf area, roots number, length, and thickness, and plant fresh and dry weights were measured. Osmotic pressure (Ψ medium, MPa) and electrical conductivity (ECmedium, mS cm-1) of media ranged from −0.2 to −0.91 MPa and 5.8 to 24 mS cm-1, respectively. Salt stress adversely affected the plant growth, and varieties differed in their responses. Progressive reduction in the studied parameters occurred as NaCl levels increased. Grouping all the varieties by cluster analysis, based on the growth parameters response to salt stress, resulted in three distinct groups: (1) salt tolerant group of two varieties, namely, Taurus and Sultana; (2) moderately salt tolerant group of four varieties, namely, Loane, Diamant, Amarin, and Sylvana; and (3) salt sensitive group of three varieties, namely, Toscana, Soraya, and Kenita. The response variation of these potato varieties under NaCl indicated the possibility of using them for developing salt tolerant varieties for production in Syria. © 2015, Tarbiat Modares University. All rights reserved.

Besher S.,National Commission for Biotechnology NCBT | Al-Ammouri Y.,National Commission for Biotechnology NCBT | Murshed R.,Damascus University
Physiology and Molecular Biology of Plants | Year: 2014

Green wild plants (dirctly before flowering) and seeds of Hyoscyamus aureus were collected from natural habitat at Al Qalamon region in Syria. Seeds were surface sterilized and cultured in vitro, after 21 days from germination stem-derived callus was induced on two different nutrient media. Tropane alkaloids were extracted from wild plants and 30 days old in vitro plants and callus, and then analyzed using GC-MS. Genetic variation was also studied between the wild and in vitro plants and the callus culture lines using twenty ISSR markers. The results showed that there were significant variations in tropane alkaloids contents between the wild plants, the in vitro plants and the callus culture lines. The highest content of hyoscyamine was in callus on line A medium, but the highest content of scopolamine was in the wild plants. However, the lowest content of tropane alkaloids was in callus on line B medium. Also the ISSR analyses showed that there was genetic variation between the wild and in vitro plants and the callus culture lines. © 2014 Prof. H.S. Srivastava Foundation for Science and Society.

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