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Yang C.-Y.,University of Sichuan | Wang J.,University of Sichuan | Zhao Y.,Ohio State University | Shen L.,University of Sichuan | And 5 more authors.
Journal of Ethnopharmacology | Year: 2010

Ethnopharmacological relevance: Panax notoginseng (Burk) F.H. Chen (Araliaceae) is a well-known and commonly used traditional Chinese herb for treatment of various diseases, such as hemostasis, edema and odynolysis. Aim of study: Our aim was to investigate the mechanisms of anti-hyperglycemic and anti-obese effects of Panax notoginseng saponins (PNS) in KK-Ay mice, and explore the components in PNS for such effects. Materials and methods: KK-Ay mice received daily intraperitoneal injections of PNS 200. mg/kg or vehicle for 30 days while ginsenoside Re 14. mg/kg, Rd 15. mg/kg, Rg1 40. mg/kg, Rb1 60. mg/kg and notoginsenoside R1 6. mg/kg for 12 days. Fasting blood glucose levels (FBGL), glucose tolerance (GT), serum insulin, leptin levels, body weight changes, food intake, adipose tissues and blood fat levels were measured at different time points. Results: The PNS group had significantly lower FBGL, improved GT and smaller body weight incremental percentage after the 30-day treatment. Additionally, Rb1 exhibited significant reduction of FBGL on day 12, and Re also exhibited a decreasing trend after the 12-day treatment. Conclusions: PNS possess anti-hyperglycemic and anti-obese activities by improving insulin- and leptin sensitivity, and Rb1 is responsible for the anti-hyperglycemic effect among the five saponins in KK-Ay mice. © 2010 Elsevier Ireland Ltd.

Jiang X.,University of Sichuan | Jiang X.,National Chengdu Center for Safety Evaluation of Traditional Chinese Medicine | Yang C.-Y.,University of Sichuan | Mao M.,University of Sichuan | And 2 more authors.
Journal of Sichuan University (Medical Science Edition) | Year: 2010

Objective: To compare the transfection efficiency of two kind of recombinant adeno-associated virus-mediated transfection to rats osteoblasts with enhanced green fluorescent protein and assess the feasibility of it as a vector for gene therapy of osteoblast lesions. Methods: The osteoblasts of rats were isolated, cultured and identified with type I collagen staged digestion method. According to different multiplicity of infection (MOI) (MOI = 1× 103,1 × 104, 1 ×105,5 × 105), rAAV-EGFP was transfected into osteoblasts with rAAV only and rAAV-ADV co-transfection respectively. The expression of EGFP along with the transfection time was observed under inverted fluorescence microscope. The transfecion efficiency and fluorescence intensity was evaluated by flow cytometry. The best MOI value was analysed and the cell growth curves were obtained according to the best MOI value to evaluate the toxical effects of tAAV-EGFP. Results: The cultured cells possessed the biological behaviors of osteoblasts. The transfecion efficiency of the rAAV was increased with the increasing of MOI. The EGFP expression reached the maximum on day 5 in ADV(-) group, the transfection efficiency of rAAVZ/6-EGFP and rAAV2/9-EGFP was 90. 2% and 66. 1% respectively when MOI was 1 X 105 and no significant increasewas observedwhen MOI was 5 × 105. In ADV(+) group, EGFP expression reached its maximum on day 3, the transfection efficiency of rAAV2/6-EGFP and rAAV2/9-EGFP was 47. 6% and 30. 5% respectively when MOI was 5×105. And no significant biologic effects on the cyto-activity was observed. Conclusion: The transfection efficiency of two kind of virus vectors was both very high and rAAV2/6's is higher than that of rAAV2/9. This suggested the Potential of rAAV-EGFP as a safe and efficient vector for Gene therapy.

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