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Oduoye O.T.,National Center for Genetic Resources and Biotechnology
Forest Science and Technology | Year: 2013

Samples from nine species of Anthocleista were collected from major extant forest and herbaria in the West African countries of Nigeria, the Republic of Benin and Ghana for identification and conservation. They were assessed in the field and in the laboratory for their morphological and anatomical characteristics. Their leaf shapes are elliptic, oblong, obovate or oblanceolate. The leaf length ranged from 8 cm to 70 cm; width was between 3 cm and 28 cm. They generally possess either anisocytic or anomocytic stomata, and two species are amphistomatic in nature. Trichomes are either stellate, dendritic or both with varying number of arms and arm length. Their leaf epidermal cuticle is coated with either soft wax or scale and plate types of ornamentation. Principal component analysis and unweighted pair group method with arithmetic average revealed that the leaf shape and size, phyllotaxy, inflorescence, epidermal cell dimension, stomata indices, trichome density and wax coating are sufficient to delimit this economically useful genus in their populations both for their medicinal importance and conservation purposes. © 2013 Korean Forest Society.

Adesoye A.I.,University of Ibadan | Emese A.,University of Ibadan | Olayode O.M.,National Center for Genetic Resources and Biotechnology
Kasetsart Journal - Natural Science | Year: 2012

Studies on in vitro organogenesis of African yam bean (Sphenostylis stenocarpa) were carried out with the aim of developing a rapid regeneration system for this crop. Embryo and leaf explants were cultured on Murashige and Skoog (MS) containing varying concentrations and combinations of 6-benzyl aminopurine (BAP), kinetin and α-naphthalene acetic acid (NAA). The maximum number of shoots per explant (4.5) and percentage multiple shoot induction (100%) were obtained in MS supplemented with 0.05 mg.L-1 NAA and 0.5 mg.L-1 BAP. The maximum shoot length (135 mm) was obtained on a medium with 1.0 mg.L-1 kinetin and 0.1 mg.L-1 NAA. When cotyledonary node explants and shoot tip explants were cultured on media with BAP and kinetin singly, each at 1.0, 1.5 and 2.0 mg.L-1, both explants produced the maximum number of shoots (4.75) and shoot length (25 mm) on 2.0 mg.L-1 BAP while the least responses were obtained on 1.0 mg.L-1 kinetin. There was no organ formation from leaves as they all produced calli. Multiple shoots from the embryo produced roots directly on shoot induction medium while shoot tip-derived multiple shoots rooted when tested on both 0.25 and 0.5 mg.L-1 NAA. Shoots from cotyledonary nodes did not produce roots. Successfully rooted plantlets obtained from this study is the first report of in vitro plant regeneration in African yam bean. This procedure for direct organ differentiation would facilitate micropropagation and improvement of this species through genetic transformation.

Borokini T.I.,National Center for Genetic Resources and Biotechnology
Tropical Conservation Science | Year: 2011

With very scanty information on invasive alien species (IAS) in Nigeria, this study was conducted to identify the invasive species in the field gene bank of the National Centre for Genetic Resources and Biotechnology (NACGRAB), Ibadan, Nigeria, on the latitude 7° 22' north of the equator and longitude 3° 50' east of the Greenwich Meridian, and also to assess the level of the species' disturbance to the conserved native plants in the protected area. This study involved sampling of the field gene bank and an on-site assessment and identification of the species and their effects on the indigenous plants established in the field for conservation. Twenty-five invasive plant species were identified, across 16 plant families, of which 14 were herbs, followed by vines, shrubs, and trees, all of which are presently estimated to occupy about 18% of the gene bank. The effects of IAS on the indigenous plants conserved in the field gene bank range from competition for space and nutrients and alteration of the tree canopies (thereby affecting the microclimatic conditions in the lower strata) to obstruction of the plants' reception of sunlight (which could thereby reduce the potential yield of the fruit trees in the gene bank. The paper also discusses the effects of IAS on biodiversity conservation in Nigeria. Human disturbance was observed as the major factor responsible for the spread of these IAS in the gene bank. The paper concludes by advocating stricter screening measures before introducing new plants into the country, capacity building on the early detection and management of IAS in protected areas for the technical staff, biological control, and exchange of technical information among concerned countries. © Temitope Israel Borokini.

Okere A.U.,National Center for Genetic Resources and Biotechnology | Adegeye A.,University of Ibadan
African Journal of Biotechnology | Year: 2011

The over exploitation of African mahogany in tropical forest has threatened the genetic base of this useful timber and medicinal tree species and as such, an experiment was conducted on the in vitro culture of Khaya grandifoliola, an endangered tree species commonly found in the high forest zones of West Africa to explore its potential for micropropagation. Embryos excised from matured seeds were cultured on Murashige and Skoog (MS) medium supplemented with naphthalene acetic acid (NAA), benzyl amino purine (BAP) and kinetine (KIN) at different concentrations. The optimum result in relation to shoot length, root length, number of nodes and number of root was obtained on MS medium supplemented with 1.0 mg/L BAP + 0.01 mg/L NAA. © 2011 Academic Journals.

Musa M.,University of Technology Malaysia | Musa M.,National Center for Genetic Resources and Biotechnology | Kikuchi A.,University of Technology Malaysia | Majid Z.A.,University of Technology Malaysia | And 2 more authors.
Jurnal Teknologi (Sciences and Engineering) | Year: 2014

The production of activated carbon (AC) from sugarcane bagasse (SCB) was carried out using central composite design of response surface method to run a limited number of experiments with the possibility of revealing the interaction of three selected factors of temperature, time and nitrogen/steam flowrate at different levels. Two second order quadratic regression model equations were developed using statistical analysis with Design Expert® software. The models were used for the prediction of removal of Cd2+ and carbon yield. Correlation coefficients (R2) were 0.957 for removal and 0.985 for yield, showing the sufficiency of the model in predicting response within 13 experimental runs. Characterization of the product with optimal performance which was produced at 900oC, with nitrogen/steam flow of 100 mL/min and activation time of 30 minutes, was carried out. The performance showed this AC sample was able to remove 62.42% Cd2+ from an aqueous solution with concentration 2 mg/L within 2 hours at optimized conditions. Experimental results indicated that AC from SCB had good prospect for Cd2+ removal. © 2014 Penerbit UTM Press. All rights reserved.

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