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Zhang Y.,National Center for Clinical Laboratory | Wang W.,National Center for Clinical Laboratory | He F.,National Center for Clinical Laboratory | Wang Z.,National Center for Clinical Laboratory | Zhong K.,National Center for Clinical Laboratory
Chinese Journal of Cardiology | Year: 2014

Objective: To analyze the sources and distribution decision limits on reference values of myocardial injury markers based on the feedback results of 150 laboratories which participated the 2012 external quality assessment (EQA) programs in China. Methods: Decision limits on myocardial injury markers from EQA programs were analyzed. Data from maternal and child care service center, children's hospital and corporations were excluded. Abnormal values and errors were eliminated. Mean, median, maximum value, minimum value, P2.5 and P97.5 were calculated. Sources of decision limits were summarized and data were filtered and only values obtained according to the reagent manufacturer's instructions as a reference source were used and grouped based on reagent characteristics. Results: According to the surveys on reference interval sources, most of reference sources were derived from reagent manufacturer's instructions and the ratio of each item was: CK-MB(μg/L)91.30%, Mb(μg/L)88.44%, cTn-I(μg/L)86.29%, cTn-T (μg/L)92.00%, CK-MB(U/L)70.65%. According to the surveys, the mean and median values of each test parameter were close to each other (CK-MB:3.74, 4.94 μg/L; Mb: 76.09, 72.00 μg/L; CTn-I:0.06, 0.09 μg/L;cTn-T:0.01, 0.02 μg/L; CK-MB:23.00, 25.00 U/L). There were significant variations on P2.5 and P97.5 distribution ranges: CK-MB 2.48-7.09 μg/L, Mb 46.00-140.03 μg/L, cTn-I 0.01-1.68 μg/L, cTn-T 0.01-12.61 μg/L, CK-MB 6.00-30.60 U/L. According to the surveys on using various reagents, there were also significant variations on P2.5 and P97.5 reagent distributions. Conclusions: The decision limit of the mean and median for each test item is close to each other while the P2.5 and P97.5 distribution of each test item consistency is poor. There is therefore an urgent need to establish an uniform reference values for cardiac markers in China.


Wang L.,Shen Zhen Mindray Bio Medical Electronics Co. | Ding J.-F.,Shen Zhen Mindray Bio Medical Electronics Co. | Ai F.,Shen Zhen Mindray Bio Medical Electronics Co. | Xie C.-F.,Shen Zhen Mindray Bio Medical Electronics Co. | And 2 more authors.
Jiliang Xuebao/Acta Metrologica Sinica | Year: 2010

It is important to establish traceability of IVD manufacturer (Mindray) γ-GT routine clinical measurement system by using gamma-glutamyltransferase(γ-GT) master calibrator with traceable value assigned by China Enzyme Reference Laboratory Network (CERLN). The γ-GT master calibrator with good commutability is used and the assigned values by IFCC primary reference measurement procedure to transfer the value to Mindray commercial product calibrator. Mindray routine measurement system calibrated by product calibrator measured 30 patient serum samples with reference value, distributing the meaningful range of γ-GT to validate the traceability. The regression equation between two methods is y=1.015 2x-0.12, r=0.999 8, the slope and intercept is no significant difference between 1 and 0 respectively, the γ-GT traceable calibration of Mindray routine measurement system was established. The China Enzyme Reference Laboratory Network plays a key role in the this process for IVD manufacturer.


Zhao S.M.,Peking Union Medical College | Wang J.X.,Peking Union Medical College | Liu H.,Beijing Hospital | Peng M.T.,National Center for Clinical Laboratory
Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology | Year: 2013

The aim of this study was to develop an ex vivo cell culture system for establishing the hematological malignancy model. Mouse bone marrow cells were transfected with GFP-expressed retroviral vectors encoding various leukemia/lymphoma-associated fusion proteins (TEL-PDGFR, Rabaptin5-PDGFR, p210BCR-ABL, AML1-ETO, NPM-ALK). After transfection, the cells were cultured in IMDM containing 10% FCS without growth factors, or with one of the following growth factor combinations: (1) murine c-kit ligand (KL) plus human flt3 ligand (FL); (2) IL-3, thrombopoietin, G-CSF, and hyper-IL-6 (3/T/G/H6); (3) KL/FL plus 3/T/G/H6. The flow cytometry was used to detect the ability of combinations of growth factors to complement the oncogene fusion protein to support self-renewal of the transfected cells. The results showed that the transfected cells could be amplified sustainably in the logarithmic growth way. The indicated combination of c-Kit ligand (KL) with flt-3 ligand (FL) supported the self-renewal of the marrow cells transfected with vectors encoding TEL-PDGFR, Rabaptin5-PDGFR, AML1-ETO and NPM-ALK, in addition to KL/FL, the self-renewal of p210 BCR-ABL transfected-marrow cells also required IL-3. The morphology of cells emerged from culture can be the predictor of the corresponding oncogene-associated malignancy. It is concluded that this study establishes a culture system ex vivo which provides a generalized method for studying hematological malignancies, and may facilitate the screening for therapeutic agents.


Bao X.,Laboratory of An Hui Chest Hospital | Wang Q.,Laboratory of An Hui Chest Hospital | Chen G.,Laboratory of An Hui Chest Hospital | Wang Q.,Anhui Medical University | And 2 more authors.
Angiology | Year: 2014

We evaluated the association between serum uric acid (SUA) levels and prehypertension in a Chinese population. A cross-sectional study was performed during 2008 to 2010, and a total of 11199 participants without hypertension or other cardiovascular diseases (CADs), aged ≥35 years, were available for analysis. After adjusting for age, alcohol consumption, smoking status, body mass index, diabetes, total cholesterol, triglycerides, serum creatinine, the odds ratios (ORs) and 95% confidence intervals (CIs) of the prehypertension from the lowest (referent) to the highest levels of SUA were 1.00 (95% CI, 0.91-1.10), 1.12 (95% CI, 1.03-1.21), 1.17 (95% CI, 1.09-1.27), and 1.25 (95% CI, 1.13-1.39; linear trend P =.002). This association persisted in subgroup analysis by gender and was also consistent with separate analysis by classification of age, smoking status, alcohol usage, overweight, and diabetes mellitus. Independent of other cardiovascular risk factors, higher SUA levels are positively associated with prehypertension in a Chinese population without hypertension and CADs. Prospective trials should evaluate interventions that lower the SUA levels. © The Author(s) 2013.


PubMed | National Center for Clinical Laboratory, Beijing Hospital and Peking Union Medical College
Type: Journal Article | Journal: Zhongguo shi yan xue ye xue za zhi | Year: 2013

The aim of this study was to develop an ex vivo cell culture system for establishing the hematological malignancy model. Mouse bone marrow cells were transfected with GFP-expressed retroviral vectors encoding various leukemia/lymphoma-associated fusion proteins (TEL-PDGFR, Rabaptin5-PDGFR, p210BCR-ABL, AML1-ETO, NPM-ALK). After transfection, the cells were cultured in IMDM containing 10% FCS without growth factors, or with one of the following growth factor combinations: (1) murine c-kit ligand (KL) plus human flt3 ligand (FL); (2) IL-3, thrombopoietin, G-CSF, and hyper-IL-6 (3/T/G/H6); (3) KL/FL plus 3/T/G/H6. The flow cytometry was used to detect the ability of combinations of growth factors to complement the oncogene fusion protein to support self-renewal of the transfected cells. The results showed that the transfected cells could be amplified sustainably in the logarithmic growth way. The indicated combination of c-Kit ligand (KL) with flt-3 ligand (FL) supported the self-renewal of the marrow cells transfected with vectors encoding TEL-PDGFR, Rabaptin5-PDGFR, AML1-ETO and NPM-ALK, in addition to KL/FL, the self-renewal of p210 BCR-ABL transfected-marrow cells also required IL-3. The morphology of cells emerged from culture can be the predictor of the corresponding oncogene-associated malignancy. It is concluded that this study establishes a culture system ex vivo which provides a generalized method for studying hematological malignancies, and may facilitate the screening for therapeutic agents.

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