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San José de las Lajas, Cuba

Rodriguez-Roche R.,Institute of Tropical Medicine | Sanchez L.,Institute of Tropical Medicine | Burgher Y.,National Center for Animal and Plant Health | Rosario D.,Institute of Tropical Medicine | And 5 more authors.
Vector-Borne and Zoonotic Diseases | Year: 2011

Dengue epidemics in Cuba have repeatedly demonstrated a month-to-month increase in clinical severity during secondary infections. The dengue 2 outbreak that occurred in Santiago de Cuba in 1997 was accompanied by the most severe intraepidemic increase in disease severity reported to date. It was initially proposed that the appearance of neutralization escape mutants during the course of the epidemic might explain this phenomenon. Recent studies have revealed that during the course of this epidemic, nucleotide substitutions appeared only in nonstructural (NS) genes, most of which were silent, except for one change in the NS1 gene. To study whether or not variation in the NS1 gene might be associated with increased disease severity during the epidemic, this gene was partially sequenced from 15 isolates obtained at different times during the 1997 epidemic. Early epidemic isolates differed from those obtained later by replacement only of threonine with serine at position 164 in the NS1 protein, an amino acid rarely found in any genotype of dengue 2 virus. All viruses isolated from patients located in Health Districts, where dengue 2 transmissions occurred late in the epidemic, contained Serine at position 164, indicating that this change was fixed within a few months. Here we argue that this single mutation contributes to viral survival or replication efficiency, resulting in enhanced infection in the presence of enhancing antibodies, a phenomenon that we term increased virus "fitness" in contrast to "virulence," an intrinsic property of the virus. © Copyright 2011, Mary Ann Liebert, Inc. Source


Rodriguez E.S.,Center for Genetic Engineering and Biotechnology | Pose A.G.,Center for Genetic Engineering and Biotechnology | Molto M.P.R.,Center for Genetic Engineering and Biotechnology | Espinoza A.S.,Center for Genetic Engineering and Biotechnology | And 2 more authors.
Biotechnology Journal | Year: 2012

The production of recombinant proteins in the milk of non-transgenic goats can be achieved by transducing the mammary gland with recombinant adenoviral vectors. However, this process involves several regulatory issues. The current study evaluates the biosafety of this production system. We present a preliminary biosafety profile based on detection of adenoviral particles in different body fluids and the antibody response after adenoviral transduction of the goat mammary gland. In addition, two methods of adenoviral inactivation in milk were tested. Although adenoviral particles were detected in the milk until day 4 after transduction, they were absent in serum, saliva, urine and feces. Anti-adenovirus antibodies were detected in serum and milk. The virus inactivation methods neutralized adenoviral particles and preserved the immunological identity of the recombinant protein. These results support the idea of a safe production of recombinant proteins using adenoviral vectors. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Source


Valera Rodriguez Y.,University of Granma | Alfonso P.,National Center for Animal and Plant Health | Ramirez W.,University of Granma | Elias A.,Institute of Veterinary Medicine IVM | And 2 more authors.
Giornale Italiano di Medicina Tropicale | Year: 2011

Orf is a highly contagious viral disease that causes important economic losses in sheep and goat farms, and constitutes zoonosis. The employment of vaccines for the control of this illness is limited and it has been associated to outbreaks because of the insufficient attenuation of the vaccine strains. Even if it is known that Orf is wide spread in sheep and goats farms in Cuba, there is still a lack of epidemiological data on this disease. Considering the limitations of effective specific preventive measures against orf this work was directed to identify the risks factors correlated to the disease, by the means of a case-control study. The risk factors for the animal population were assessed in order to identify additional means to control the disease. A total of ten flocks randomly selected were investigated. For gathering information a survey was carried out to identify the presumed risk factors. The survey allowed us to identify the risk factors significantly associated to the disease. The strength of the identified associations and etiological ratio at individual and population level, evidenced a real possibility to significantly reduce the orf incidence through an improvement of handling procedures. Source


Perez K.A.,Las Tunas University | Pinol B.,National Center for Animal and Plant Health | Rosete Y.A.,Rothamsted Research | Rosete Y.A.,CABI Inc | And 3 more authors.
Journal of Phytopathology | Year: 2010

Transmission tests were conducted with field-collected Bunchy Top Symptoms (BTS) phytoplasma-infected specimens of Empoasca papayae. BTS developed in all eight inoculated papayas 3 months later. The BTS phytoplasma was identified in six of eight inoculated papayas, whose partial 16S rRNA sequence (GenBank Accession no. FJ6492000) was 99.9% identical with those from the collected papayas (GenBank Accession no FJ649198) and E. papayae (GenBank Accession no. FJ649199), all of which are members of group 16SrII, '. Candidatus Phytoplasma aurantifolia'. Results confirmed the ability of E. papayae to transmit the BTS phytoplasma. © 2009 Blackwell Verlag GmbH. Source


Ramos O.S.,University of Concepcion | Pose A.G.,Center for Genetic Engineering and Biotechnology | Gomez-Puerta S.,University of Concepcion | Gomez J.N.,National Center for Animal and Plant Health | And 5 more authors.
Comparative Immunology, Microbiology and Infectious Diseases | Year: 2011

Recombinant adenoviral vectors have emerged as an attractive system for veterinary vaccines development. However, for poultry vaccination a very important criterion for an ideal vaccine is its low cost. The objective of this study was to test the ability of chicken CD154 to enhance the immunogenicity of an adenoviral vector-based vaccine against avian influenza virus in order to reduce the amount of antigen required to induce an effective immune response in avian. Chickens were vaccinated with three different doses of adenoviral vectors encoding either HA (AdHA), or HA fused to extracellular domain chicken's CD154 (AdHACD). Hemagglutination inhibition (HI) assay and relative quantification of IFN-γ showed that the adenoviral vector encoding for the chimeric antigen is able to elicit an improved humoral and cellular immune response, which demonstrated that CD154 can be used as a molecular adjuvant allowing to reduce in about 50-fold the amount of adenoviral vector vaccine required to induce an effective immune response. © 2010 Elsevier Ltd. Source

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