Kumar R.,ICAR National Bureau of Fish Genetics Resources |
Baisvar V.S.,ICAR National Bureau of Fish Genetics Resources |
Kushwaha B.,ICAR National Bureau of Fish Genetics Resources |
Waikhom G.,Institute of Bioresources and Sustainable Development |
Nagpure N.S.,ICAR National Bureau of Fish Genetics Resources
Nucleus (India) | Year: 2017
The cytogenetic investigations of Cyprinus carpio, collected from river Gomti, Lucknow, India, was undertaken in the present study using giemsa/silver nitrate/chromomycin A3 staining and 18S rDNA probe hybridization on the chromosomes. The metaphase chromosome compliment analyses revealed the presence of diploid chromosome number 100. The karyotype consisted of 24m+26sm+18st+32t chromosomes. The silver nitrate stained nucleolar organizer regions were found at terminal position on the short arms of two sub-metacentric homologous chromosomes, and the chromomycin A3 positive sites suggested abundance of GC-rich repetitive DNAs in this region. The fluorescence in situ hybridization confirmed the location of 18S rDNA probe on one pair of metaphase chromosomes. Negative-DAPI staining of NORs indicated lack of AT-rich sequence in this region. The cytogenetic evidence of polyploidization reported previously in C. carpio has been proved using molecular cytogenetic approach in this study leaving behind little or no contradiction. The FISH technique with 18S rDNA probe, performed first time in this species, showed the importance of biomarkers in using phylogenetic and evolutionary studies useful in taxonomy and conservation of C. carpio. © 2016, Archana Sharma Foundation of Calcutta.
Behera B.K.,ICAR Central Inland Fisheries Research Institute |
Kumari K.,ICAR Central Inland Fisheries Research Institute |
Baisvar V.S.,ICAR Central Inland Fisheries Research Institute |
Rout A.K.,ICAR Central Inland Fisheries Research Institute |
And 3 more authors.
Mitochondrial DNA | Year: 2015
In the present study, the complete mitochondrial genome sequence of Labeo gonius is reported using PGM sequencer (Ion Torrent). The complete mitogenome of L. gonius is obtained by the de novo sequences assembly of genomic reads using the Torrent Mapping Alignment Program (TMAP) which is 16 614 bp in length. The mitogenome of L. gonius comprised of 13 protein-coding genes, 22 tRNAs, 2 rRNA genes, and D-loop as control region along with gene order and organization, being similar to most of other fish mitogenomes of NCBI databases. The mitogenome in the present study has 99% similarity to the complete mitogenome sequence of Labeo fimbriatus, as reported earlier. The phylogenetic analysis of Cypriniformes depicted that their mitogenomes are closely related to each other. The complete mitogenome sequence of L. gonius would be helpful in understanding the population genetics, phylogenetics, and evolution of Indian Carps. © 2015 Taylor & Francis.
PubMed | ICAR National Bureau of Fish Genetics Resources and Indian Central Institute of Freshwater Aquaculture
Type: Journal Article | Journal: Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis | Year: 2016
In the present study, the complete mitochondrial genome sequence of Heteropneustes fossilis is reported using massive parallel sequence technology. The complete mitogenome of H. fossilis is obtained by de novo assembly of paired end Illumina sequences using CLC Genomics Workbench version 7.0.4, which is 16,489bp in length. It comprised of 13 protein- coding genes, 22 tRNAs, 2 rRNA genes and a putative control region along with the gene order and organization, being similar to most of vertebrates. The mitogenome in the present study has 99% similarity to the complete mitogneome sequence of H. Fossilis, as reported earlier. Phylogenetic analysis of Siluriformes depicted that Heteropneustids were closer to Clariids. The mitogenome sequence of H. fossilis contributes better understanding of population genetics, phylogenetics and evolution of Indian catfish species.