National Bureau of Agriculturally Important Microorganisms NBAIM

Mau, India

National Bureau of Agriculturally Important Microorganisms NBAIM

Mau, India
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Yandigeri M.S.,National Bureau of Agriculturally Important Microorganisms NBAIM
Mikrobiologiia | Year: 2011

Two diazotrophic cyanobacteria, Westiellopsis prolifica and Anabaena variabilis were evaluated for elucidating the possible mechanism of mineral phosphate solubilization. Phosphate starved cyanobacteria evaluated for the presence of organic acids, extracellular compounds or enzymes that might have been produced and promoted the mineral phosphate solubilization with Mussorie Rock Phosphate and Tricalcium Phosphate as substrates. Both the cultures did not reveal production of organic acids throughout the incubation period when checked for decrease in pH of the media and thin layer chromatography Thin layer chromatography of culture filtrates showed the presence of hydrocarbon like compound. Further analysis of the culture filtrates with gas liquid chromatography, a single peak near to the retention time of 7.6 was observed in all extracts of culture filtrates irrespective of phosphate source. UV-visible spectra of culture filtrates revealed the absorption maxima of 276 nm. Gas Chromatographic-Mass Spectrometric analysis of culture filtrates showed most intense peak in the electron impact (EI) ionization was at m/z 149 and molecular ion peaks at m/z 207 and 167, inferring the presence of phthalic acid. Among the mechanisms in mineral phosphate solubilization, it was evident that these cyanobacteria used phthalic acid as possible mode of P solubilization.


Sahay H.,National Bureau of Agriculturally Important Microorganisms NBAIM | Babu B.K.,National Bureau of Agriculturally Important Microorganisms NBAIM | Babu B.K.,Indian International Crops Research Institute for the Semi Arid Tropics | Singh S.,Rani Durgavati University | And 3 more authors.
Journal of Basic Microbiology | Year: 2013

Microorganisms, native to the cold environments have successfully acclimatized their physiological, metabolic, and biological features, exhibiting uniqueness in their enzymes, proteins, and membrane structures. These cold-active enzymes have immense biotechnological potential. The diversity of culturable bacteria in two different water lakes (the sub-glacial freshwater and the brackish) of Himalayas was analyzed using SYBR green staining and cultural methods. A total of 140 bacteria were isolated and were grouped as psychrophiles, psychrotrophs, and psychrotolerant organisms, based on their optimal temperature for growth. The amplified ribosomal DNA restriction analysis using three restriction enzymes facilitated the grouping of these isolates into 96 genotypes at ≥85% polymorphism. Phylogenetic analysis using 16S rRNA gene sequences revealed that the bacterial strains from both lakes belonged to Firmicutes, Proteobacteria (α, β, and γ) or Actinobacteria. Screening of the germplasm for the activity of different cold-active hydrolases such as protease, amylase, xylanase, and cellulase, revealed that about 16 isolates were positive, and exhibiting a wide range of stability at various temperature and pH. Our results suggest that the distinctly different ecosystems of sub-glacial freshwater and brackish water lakes have diverse groups of bacteria, which can be an excellent source of extracellular hydrolases with a wide range of thermal stability. © 2013.


Yandigeri M.S.,National Bureau of Agriculturally Important Microorganisms NBAIM | Yadav A.K.,National Bureau of Agriculturally Important Microorganisms NBAIM | Meena K.K.,National Bureau of Agriculturally Important Microorganisms NBAIM | Pabbi S.,Indian Agricultural Research Institute
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2010

The nitrogen fixing cyanobacterial strains namely Anabaena variabilis (Nostocales, Nostocaceae) and Westiellopsis prolifica (Nostocales, Hapalosiphonaceae) were evaluated for their nitrogen fixation and growth potential in response to different concentrations (10, 20 and 30 mg P) of the alternate insoluble P-sources Mussorie Rock Phosphate and Tricalcium Phosphate. Distinct and significant intergeneric differences were observed with respect to nitrogen fixation measured as Acetylene Reduction Activity (ARA) and growth potential as soluble proteins, total carbohydrate content, dry weight and total chlorophyll content in response to different concentrations of Mussorie Rock Phosphate and Tricalcium Phosphate. Both the strains showed higher soluble protein content at 20 mg P (Mussorie Rock Phosphate) that increased with time of incubation in A. variabilis. Both cyanobacteria recorded maximum Acetylene Reduction Activity at 20 mg P (Tricalcium Phosphate) followed by activity in presence of soluble phosphate (K2HPO4). The mean activity at all concentrations of insoluble phosphate (Mussorie Rock Phosphate and Tricalcium Phosphate) was more than in the presence of soluble phosphate. © 2010 Springer Science+Business Media B.V.


Kashyap P.L.,National Bureau of Agriculturally Important Microorganisms NBAIM | Kumar S.,National Bureau of Agriculturally Important Microorganisms NBAIM | Srivastava A.K.,National Bureau of Agriculturally Important Microorganisms NBAIM | Sharma A.K.,National Bureau of Agriculturally Important Microorganisms NBAIM
World Journal of Microbiology and Biotechnology | Year: 2013

Myconanotechnology is an emerging field, where fungi can be harnessed for the synthesis of nanomaterials or nanostructures with desirable shape and size. Though myconanotechnology is in its infancy, potential applications provide exciting waves of transformation in agriculture and fascinate microbiologists and other researchers to contribute in providing incremental solutions through green chemistry approaches for advancing food security. In this article, we provide a brief overview of the research efforts on the mycogenic synthesis of nanoparticles with particular emphasis on mechanisms and potential applications in agriculture and allied sectors. © 2012 Springer Science+Business Media Dordrecht.


Kumar S.,National Bureau of Agriculturally Important Microorganisms NBAIM | Singh R.,National Bureau of Agriculturally Important Microorganisms NBAIM | Kashyap P.L.,National Bureau of Agriculturally Important Microorganisms NBAIM | Srivastava A.K.,National Bureau of Agriculturally Important Microorganisms NBAIM
Scientia Horticulturae | Year: 2013

Early detection of infection is very crucial for efficient management of early blight of tomato caused by Alternaria solani. To monitor and quantify the occurrence of this fungus, a diagnostic tool based on real-time PCR was developed. Specific-primers were designed from β-tubulin gene and specificity was checked with A. solani isolates and related species obtained from different geographical origins. The primers were highly specific for A. solani, as no amplification signal was observed from thirteen other closely related taxa. Primer set, AS1 (5'-GCTCCCACTCCTTCCGCGC-3') and AS2 (5'-GGAGGTGGAGTTACCGACAA-3') amplified a specific amplicon of 289. bp from all A. solani isolates. The lowest detection limit of the real-time PCR assay with designed primer set (AS1 and AS2) was 0.5. pg. The assay was also successfully validated on artificially infested tomato seedlings and able to detect A. solani up to 20 days post inoculation. To the best of our knowledge, this is the first report of a quantitative real time PCR detection method for rapid and specific detection of A. solani with a primer set designed from β-tubulin region. © 2013.


Srivastava A.K.,National Bureau of Agriculturally Important Microorganisms NBAIM | Singh P.,National Bureau of Agriculturally Important Microorganisms NBAIM | Singh R.K.,National Bureau of Agriculturally Important Microorganisms NBAIM | Kashyap P.L.,National Bureau of Agriculturally Important Microorganisms NBAIM | And 3 more authors.
World Journal of Microbiology and Biotechnology | Year: 2014

The indigenous fungal flora of three oil refinery contaminated sites (Bharuch, Valsad and Vadodara) of India has been documented in the present investigation. A total seventy-five fungal morphotypes were isolated from these sites and out of them, only fifteen isolates were capable of utilizing ethanol (0-8 %; v:v) as a sole source of carbon and energy for growth. Ten percent ethanol was completely lethal for the growth of all the isolated fungus. Biochemical characterization of the potent ethanol utilizing fungal isolates was studied based on substrate utilization profiles using BIOLOG phenotype microarray plates. Based on the morphological characters and Internal Transcribed Spacer region of ribosomal DNA, the fungal isolates were identified as Fusarium brachygibbosum, Fusarium equiseti, Fusarium acuminatum, Pencillium citrinum, Alternaria tenuissima, Septogloeum mori, Hypocrea lixii, Aureobasidium sp., Penicillium sp., and Fusarium sp. Intra-species genetic diversity among Fusarium sp. was evaluated by whole genome analysis with repetitive DNA sequences (ERIC, REP and BOX) based DNA fingerprinting. It was found that these fungus use alcohol dehydrogenase and acetaldehyde dehydrogenase enzymes based metabolism pathway to utilize ethanol for their growth and colonization. © 2013 Springer Science+Business Media Dordrecht.


Kumar S.,National Bureau of Agriculturally Important Microorganisms NBAIM | Rai S.,National Bureau of Agriculturally Important Microorganisms NBAIM | Maurya D.K.,National Bureau of Agriculturally Important Microorganisms NBAIM | Kashyap P.L.,National Bureau of Agriculturally Important Microorganisms NBAIM | And 2 more authors.
Phytoparasitica | Year: 2013

Expressed sequence tags (ESTs) are the source of simple sequence repeats (SSRs) that can be used to develop molecular markers for the study of polymorphism and genetic diversity. In the present investigation, 30 EST simple sequence repeats (SSR) primer sets derived from three formae speciales of Fusarium oxysporum: melonis (Fom), cucumerium (Foc), and lycopersici (Fol) - were tested for transferability to Fusarium udum. The majority of SSR loci contain trinucleotide (63.70%) while fewer contain di- (27.41%), tetra- (5.64%) and penta-nucleotide (3.22%) repeats. The number of alleles at these SSR loci ranged from one to three, with an average of 1.4 alleles per locus. CAG (24.19%) and AC (16.93%) were the most abundant motifs identified. Three markers (FomSSR-8, FolSSR-2 and FolSSR-4) were found highly informative for genetic characterization of F. udum and very useful in distinguishing the polymorphism rate of the markers at specific locus; however, polymorphic information content (PIC) was maximum (0.597) in FocSSR-7. In terms of cross species transferability, 70% of the primer sets of Fom-SSR and Fol-SSR and 30% of the Foc-SSR produced an amplicon in F. udum isolates. To the best of our knowledge, this is the first set of EST SSR markers developed and assessed for the variability, genetic analysis and evolutionary relationships of the F. udum population. © 2013 Springer Science+Business Media Dordrecht.


Babu B.K.,National Bureau of Agriculturally Important Microorganisms NBAIM | Mesapogu S.,National Bureau of Agriculturally Important Microorganisms NBAIM | Sharma A.,National Bureau of Agriculturally Important Microorganisms NBAIM | Somasani S.R.,National Bureau of Agriculturally Important Microorganisms NBAIM | Arora D.K.,National Bureau of Agriculturally Important Microorganisms NBAIM
Mycologia | Year: 2011

A real-time qPCR assay was developed to detect and quantify Macrophomina phaseolina abundance in rhizosphere soil and plant tissue. Both TaqMan and SYBR green techniques were targeted on, 1 kb sequence characterized amplified region (SCAR) of M. phaseolina and two sets of specific primers were designed for SYBR green (MpSyK) and TaqMan (MpTqK) assays. No cross-hybridization and no fluorescent signal exceeding the baseline threshold was observed in TaqMan and SYBR green assays, respectively. The minimum detection limit or sensitivity of TaqMan assay was 30 fg/μL of M. phaseolina DNA and limit of quantification of M. phaseolina viable population was estimated as 0.66 × 10 5 CFU/g soil -1 equivalent to 10 pg/μL of target DNA. This is the first report which demonstrated real-time qPCR assays with greater specificity and sensitivity to detect M. phaseolina population in soil and plant materials. © 2011 by The Mycological Society of America.


Ray S.,Indian Central Rice Research Institute | Chakdar H.,National Bureau of Agriculturally Important Microorganisms NBAIM
Molecular Genetics, Microbiology and Virology | Year: 2014

Since last two decades, the role of structural fluctuations of RNA molecules have emerged as one of the key aspects for gene expression in bacteria and they have been found to play very crucial roles for survival of bacteria under highly fluctuating environmental conditions. Riboswitches are one of the RNA elements located in the 5′ region of bacterial mRNA controlling the expression of a gene located downstream to it, by conformational changes of its own upon selective binding to ligands. These molecular fossils are probably the most ancient regulatory system for gene expression. Association of riboswitches with bacterial pathogenesis and other related functions has attracted their exploitation as potential drug targets. Natural as well as synthetic riboswitches hold considerable potential to be the next generation gene control systems to be used in the field of molecular biology and genetic engineering. © 2014, Allerton Press, Inc.


Razali N.,University Technology of MARA | Agarwal R.,University Technology of MARA | Agarwal P.,University Technology of MARA | Kumar S.,International Medical University | And 4 more authors.
Clinical and Experimental Ophthalmology | Year: 2015

Background: Steroid-induced ocular hypertension is currently treated in the same way as primary open-angle glaucoma. However, the treatment is often suboptimal and is associated with adverse effects. We evaluated the oculohypotensive effects of topical trans-resveratrol in rats with steroid-induced ocular hypertension and involvement of adenosine receptors (AR) in intraocular pressure (IOP) lowering effect of trans-resveratrol. Methods: The oculohypotensive effect of unilateral single-drop application of various concentrations of trans-resveratrol was first studied in oculonormotensive rats. Concentration with maximum effect was similarly studied in rats with steroid-induced ocular hypertension. Involvement of AR was studied by observing the alterations of IOP in response to trans-resveratrol after pretreating animals with AR subtype-specific antagonists. Additionally, we used computational methods, including 3D modelling, 3D structure generation and protein-ligand interaction, to determine the AR-trans-resveratrol interaction. Results: All concentrations of trans-resveratrol produced significant IOP reduction in normotensive rat eyes. Maximum mean IOP reduction of 15.1% was achieved with trans-resveratrol 0.2%. In oculohypertensive rats, trans-resveratrol 0.2% produced peak IOP reduction of 25.2%. Pretreatment with A1 antagonist abolished the oculohypotensive effect of trans-resveratrol. Pretreatment with A3 and A2A AR antagonists produced significant IOP reduction in both treated and control eyes, which was further augmented by trans-resveratrol application in treated eyes. Computational studies showed that trans-resveratrol has highest affinity for A2B and A1, followed by A2A and A3 AR. Conclusion: Topically applied trans-resveratrol reduces IOP in rats with steroid-induced ocular hypertension. Trans-resveratrol-induced oculohypotension involves its agonistic activity at the A1 AR. © 2014 Royal Australian and New Zealand College of Ophthalmologists.

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