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Singh K.P.,Indian Institute of Toxicology Research | Gupta S.,Indian Institute of Toxicology Research | Singh A.K.,Indian Institute of Toxicology Research | Sinha S.,National Botanical Research Institute
Journal of Hazardous Materials | Year: 2011

A magnetic nanocomposite was developed and characterized. Adsorption of crystal violet (CV) dye from water was studied using the nanocomposite. A four-factor central composite design (CCD) combined with response surface modeling (RSM) was employed for maximizing CV removal from aqueous solution by the nanocomposite based on 30 different experimental data obtained in a batch study. Four independent variables, viz. temperature (10-50 °C), pH of solution (2-10), dye concentration (240-400. mg/l), and adsorbent dose (1-5. g/l) were transformed to coded values and a second-order quadratic model was built to predict the responses. The significance of independent variables and their interactions were tested by the analysis of variance (ANOVA) and t-test statistics. Adequacy of the model was tested by the correlation between experimental and predicted values of the response and enumeration of prediction errors. Optimization of the process variables for maximum adsorption of CV by nanocomposite was performed using the quadratic model. The Langmuir adsorption capacity of the adsorbent was determined as 81.70. mg/g. The model predicted maximum adsorption of 113.31. mg/g under the optimum conditions of variables (concentration 240. mg/l; temperature 50 °C; pH 8.50; dose 1. g/l), which was very close to the experimental value (111.80. mg/g) determined in batch experiment. © 2010 Elsevier B.V. Source

Pandey V.C.,National Botanical Research Institute | Singh N.,National Botanical Research Institute
Agriculture, Ecosystems and Environment | Year: 2010

Fly ash (FA)-a coal combustion residue of thermal power plants has been regarded as a problematic solid waste all over the world. The conventional disposal methods for FA lead to degradation and contamination of the arable land. However, several studies proposed that FA can be used as a soil-additive that may improve physical, chemical and biological properties of the degraded soils and is a source of readily available plant micro- and macro-nutrients. Numerous studies revealed that the lower FA incorporation in soil modifies the physico-chemical, biological and nutritional quality of the soil. However, the higher dosage of FA incorporation results in heavy metal pollution and hinders the microbial activity. Practical value of FA in agriculture as an "eco-friendly and economic" fertilizer or soil amendments can be established after repeated field experiments for each type of soil to confirm its quality and safety. Integrated Organic/Biotechnological approaches should be applied for the reducing toxicity of FA contaminated site near thermal power plants. Overall, study reveals that FA could be effectively used in the barren or sterile soil for improving quality and enhancing fertility. The purpose of this paper is to explore the possibility of FA addition into degraded soils for improving nutritional and physico-chemical properties. © 2009 Elsevier B.V. All rights reserved. Source

Pandey V.C.,Babasaheb Bhimrao Ambedkar University | Singh K.,National Botanical Research Institute
Ecological Engineering | Year: 2011

The present article briefly describes the concerns regarding the suitability of Vigna radiata L. for the revegetation of fly ash landfills. © 2011 Elsevier B.V. Source

Pandey V.C.,Babasaheb Bhimrao Ambedkar University | Singh K.,National Botanical Research Institute | Singh R.P.,Babasaheb Bhimrao Ambedkar University | Singh B.,National Botanical Research Institute
Ecological Engineering | Year: 2012

Increasing areas of coal fly ash (FA) deposit pose a serious concern for its safe disposal to maintain the environment quality. Several thousand hectares of land have been occupied for the storage of FA all over the world. FA deposits cause serious environmental pollution through wind erosion and ground water contamination through leaching process. Naturally growing Saccharum munja on the derelict FA lagoons of NTPC Unchahar, India was identified as a native perennial grass in the rehabilitation process. We tested its ecological suitability for the effective restoration of FA lagoons in terms of their abundance and stabilization. The FA contains alkaline pH, and low organic C coupled with toxic metals i.e. Fe, Cd, Cr, Cu, Mn, Ni, Pb and Zn. The concentrations of these metals in different parts of the plant fall in the average range of plants and were found within toxic limits. Natural colonization of S. munja predominate in the areas of FA deposits with fast growth and high biomass which is used by local people for making ropes, baskets, mats, huts, etc. to support their livelihood. These results suggest that S. munja would be potentially useful to rehabilitate the FA dumps more efficiently if it is introduced properly on fresh lagoons to convert barren FA deposits into ecologically and socio-economically productive habitats without any inputs or maintenance. © 2011 Elsevier B.V.. Source

Chaudhry V.,National Botanical Research Institute | Nautiyal C.S.,National Botanical Research Institute
Bioresource Technology | Year: 2011

A novel PA Medium (PAM) for efficient screening of phosphate-accumulating organisms (PAOs) was developed taking Serratia marcescens NBRI1213 as model organism. The defined National Botanical Research Institute's growth medium (NBRI) supplemented with 0.1% maltose, designed for quantitative estimation of phosphate accumulation was designated as PAM. Our work suggested usage of PAM for efficient qualitative screening and as a microbiological medium for preferential selection of PAOs on Petri-plates. For qualitative screening of PAOs, Toluidine blue-O dye (TBO) was supplemented in PAM, designated as PAM-TBO. Qualitative analysis of phosphate accumulated by various groups correlated well with grouping based upon quantitative analysis of PAOs, effect of carbon, nitrogen, salts, and phosphate accumulation-defective transposon mutants. For significantly increasing sample throughput, efficiency of screening PAOs was further enhanced by adaptation of PAM-TBO assay to microtiter plate based method. It is envisaged that usage of this medium will be salutary for quick screening of PAOs from environment. © 2011 Elsevier Ltd. Source

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