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Mechergui A.,National Bone Marrow Transplant Center | Mechergui A.,Center National Of Greffe Of Moelle Osseuse | Achour W.,National Bone Marrow Transplant Center | Ben Hassen A.,National Bone Marrow Transplant Center
Journal of Molecular Microbiology and Biotechnology | Year: 2015

We analyzed 85 Neisseria spp. strains collected by swabbing from neutropenic patients to determine the prevalence of reduced susceptibility to penicillin and to ascertain the clonal relationship between these strains. High genetic diversity and an elevated level of penicillin resistance were found among commensal Neisseria clinical isolates. © 2015 S. Karger AG, Basel. Source


Khadraoui N.,Laboratory of Parasitology Mycology | Kallel K.,Laboratory of Parasitology Mycology | Bouchami O.,National Bone Marrow Transplant Center | Bouchakoua M.,Laboratory of Parasitology Mycology | And 5 more authors.
Annales de Biologie Clinique | Year: 2011

Candida albicans is the most important cause of fungal infections in intensive care units. The aim of this work was to compare the profiles of C. albicans in order to specify their genetic polymorphism and to determine the origin of these infections. Thirty-five C. albicans strains were collected from different clinical samples of 12 patients and three health-workers in an intensive care unit (ICU) in Rabta hospital of Tunisia, between August 2007 and April 2008. After digestion with BssHII, the isolates were typed by pulsed field gel electrophoresis (PFGE). The PFGE profiles were analyzed using a visual method, which showed three PFGE types (A, B and C) and the dendrogram generated three clusters (clusters I to III). An average similarity coefficient of 0.83, suggests that isolates are related. Copyright 2010 American Association for Clinical Chemistry, Inc. Source


Khadraoui N.,Laboratory of Parasitology Mycology | Kallel K.,Laboratory of Parasitology Mycology | Bouchami O.,Intensive Care Unit | Bouchakoua M.,Laboratory of Parasitology Mycology | And 6 more authors.
Journal de Mycologie Medicale | Year: 2010

Objective: The aim of our study was to compare some Candida tropicalis clinical isolates in order to specify their genetic polymorphism. Patients: The isolates were collected between August 2007 and April 2008 from 15 patients hospitalized in an intensive care unit in Rabta hospital, Tunisia. Materials and methods: All C. tropicalis isolates collected from patients hospitalized in this hospital unit were typed using pulsed field gel electrophoresis (PFGE). Results: During the study period, 15 patients revealed to be positive for C. tropicalis and 34 isolates were collected. Among them, 29 isolates belonged to the same PFGE type designated A (with three subtypes A1, A2 and A3), while two isolates belonged to the PFGE type B and the remaining three strains to the PFGE type C. Conclusion: The majority of isolates belonged to the same PFGE type (85%) but the subtype A1 was more represented than the subtypes A2 and A3. © 2010 Elsevier Masson SAS. Source

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