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Habu J.B.,National Biotechnology Development Agency | Ibeh B.O.,Michael Okpara University of Agriculture | Ibeh B.O.,National Biotechnology Development Agency
Biological Research | Year: 2015

Background: The aim of the present study was to evaluate the in vitro antioxidant and free radical scavenging capacity of bioactive metabolites present in Newbouldia laevis leaf extract. Results: Chromatographic and spectrophotometric methods were used in the study and modified where necessary in the study. Bioactivity of the extract was determined at 10 μg/ml, 50 μg/ml, 100 μg/ml, 200 μg/ml and 400 μg/ml concentrations expressed in % inhibition. The yield of the ethanolic leaf extract of N.laevis was 30.3 g (9.93%). Evaluation of bioactive metabolic constituents gave high levels of ascorbic acid (515.53 ± 12 IU/100 g [25.7 mg/100 g]), vitamin E (26.46 ± 1.08 IU/100 g), saponins (6.2 ± 0.10), alkaloids (2.20 ± 0.03), cardiac glycosides(1.48 ± 0.22), amino acids and steroids (8.01 ± 0.04) measured in mg/100 g dry weight; moderate levels of vitamin A (188.28 ± 6.19 IU/100 g), tannins (0.09 ± 0.30), terpenoids (3.42 ± 0.67); low level of flavonoids (1.01 ± 0.34 mg/100 g) and absence of cyanogenic glycosides, carboxylic acids and aldehydes/ketones. The extracts percentage inhibition of DPPH, hydroxyl radical (OH.), superoxide anion (O.-2), iron chelating, nitric oxide radical (NO), peroxynitrite (ONOO−), singlet oxygen (1O2), hypochlorous acid (HOCl), lipid peroxidation (LPO) and FRAP showed a concentration-dependent antioxidant activity with no significant difference with the controls. Though, IC50 of the extract showed significant difference only in singlet oxygen (1O2) and iron chelating activity when compared with the controls. Conclusions: The extract is a potential source of antioxidants/free radical scavengers having important metabolites which maybe linked to its ethno-medicinal use. © 2015 Habu and Ibeh; licensee BioMed Central.


PubMed | University of Witwatersrand, University of Sheffield, Sanguine, Future University of Sudan and 27 more.
Type: Journal Article | Journal: Genome research | Year: 2016

The application of genomics technologies to medicine and biomedical research is increasing in popularity, made possible by new high-throughput genotyping and sequencing technologies and improved data analysis capabilities. Some of the greatest genetic diversity among humans, animals, plants, and microbiota occurs in Africa, yet genomic research outputs from the continent are limited. The Human Heredity and Health in Africa (H3Africa) initiative was established to drive the development of genomic research for human health in Africa, and through recognition of the critical role of bioinformatics in this process, spurred the establishment of H3ABioNet, a pan-African bioinformatics network for H3Africa. The limitations in bioinformatics capacity on the continent have been a major contributory factor to the lack of notable outputs in high-throughput biology research. Although pockets of high-quality bioinformatics teams have existed previously, the majority of research institutions lack experienced faculty who can train and supervise bioinformatics students. H3ABioNet aims to address this dire need, specifically in the area of human genetics and genomics, but knock-on effects are ensuring this extends to other areas of bioinformatics. Here, we describe the emergence of genomics research and the development of bioinformatics in Africa through H3ABioNet.


PubMed | DuPont Pioneer, University of Nigeria, Kenya Agricultural Research Institute, Africa Harvest Biotechnology Foundation International and National Biotechnology Development Agency
Type: Journal Article | Journal: The Proceedings of the Nutrition Society | Year: 2015

The focus of the review paper is to discuss how biotechnological innovations are opening new frontiers to mitigate nutrition in key agricultural crops with potential for large-scale health impact to people in Africa. The general objective of the Africa Biofortified Sorghum (ABS) project is to develop and deploy sorghum with enhanced pro-vitamin A to farmers and end-users in Africa to alleviate vitamin A-related micronutrient deficiency diseases. To achieve this objective the project technology development team has developed several promising high pro-vitamin A sorghum events. ABS 203 events are so far the most advanced and well-characterised lead events with about 12 g -carotene/g tissue which would supply about 40-50 % of the daily recommended vitamin A at harvest. Through gene expression optimisation other events with higher amounts of pro-vitamin A, including ABS 214, ABS 235, ABS 239 with 25, 30-40, 40-50 g -carotene/g tissue, respectively, have been developed. ABS 239 would provide twice recommended pro-vitamin A at harvest, 50-90 % after 3 months storage and 13-45 % after 6 months storage for children. Preliminary results of introgression of ABS pro-vitamin A traits into local sorghum varieties in target countries Nigeria and Kenya show stable introgression of ABS vitamin A into local farmer-preferred sorghums varieties. ABS gene Intellectual Property Rights and Freedom to Operate have been donated for use royalty free for Africa. Prior to the focus on the current target countries, the project was implemented by fourteen institutions in Africa and the USA. For the next 5 years, the project will complete ABS product development, complete regulatory science data package and apply for product deregulation in target African countries.


PubMed | National Biotechnology Development Agency, Adekunle Ajasin University and Korea Research Institute of Bioscience and Biotechnology
Type: Journal Article | Journal: Journal of molecular modeling | Year: 2015

As a key step in achieving low-cost, easily accessible anti-cancer therapy for low- and middle-income countries, we recently established the scientific basis for the folkloric use of Artocarpus altilis for the treatment of cancer by investigating the geranyl dihydrochalcone (CG-901) content and its interference with signal transducer and activator of transcription 3 (STAT3) phosphorylation and blockage of further downstream signaling. In the current study, the CG-901 upstream target was queried by chemical fingerprinting similarity assessment, semi-empirical (PM6ESCF) QMMM and molecular dynamics (MD) simulation. Moderate (0.4) to high (0.7) Tanimoto scores were found when the CG-901 scaffold was compared to ligands co-crystallized with Janus kinases (JAK) 1-3. High negative energy values were obtained when the CG-901 was treated semi-empirically (PM6ESCF) within the classical field of JAK (1-3). Multiple nanosecond MD simulations showed that CG-901 did not cause any large structural perturbations in the nucleotide-binding, activation and catalytic loops within the kinase (JH1) domain of JAK (1-3); however, it reduced the energy required to attain metastability along the path to energy minima conformation. In comparison to JAK1 and Apo-state JAK2, JAK2-bound CG-901 exhibited a highly re-organized key intra-domain protein network; indicating atomic level interference with inter-residue communication. In conclusion, CG-901 isolated from A. altilis represents a broad-spectrum JAK inhibitor, which may underlie the mechanism of STAT3 phosphorylation blockage. Graphical abstract Upper panel Janus kinase 2 upstream signaling pathway. Lower panel Apo-JAK2 (left) and CG-901-bound JAK2 (right).


Abioye O.P.,Minna Federal University Of Technology | Iroegu V.T.,National Biotechnology Development Agency | Aransiola S.A.,National Biotechnology Development Agency
Journal of Environmental Science and Technology | Year: 2015

The release of azo dyes especially methyl red into the environment is of great concern due to coloration of natural water, toxicity, mutagenicity and their biotransformation product. Economical and bio-friendly approaches are needed to remediate dye contaminated waste water from various industries. In this study, Staphylococcus aureus capable of degrading azo dye was isolated from waste dump site. The isolate was studied for their ability to decolorize Methyl Red (MR) using UV spectrophotometer at 430 nm at 3 different concentrations (250, 500 and 750 mL) under aerobic condition for the period of 12 days. The 62, 58 and 50% methyl red decolorizations were recorded in 750, 500 and 250 mL dilution respectively at the end of 12 day. The results of this study suggest the potential of Staphylococcus aureus for the treatment of waste water containing methyl red. © 2015 Asian Network for Scientific Information.


Abioye O.P.,Minna Federal University Of Technology | Afolayan E.O.,Minna Federal University Of Technology | Aransiola S.A.,National Biotechnology Development Agency
Research Journal of Environmental Toxicology | Year: 2015

A study was designed to assess the efficacy of yeast isolated from spoilt water melon in the biological treatment of pharmaceutical effluent. Two yeast species were identified as Saccharomyces cerevisiae and Torulaspora delbrueckii. Each of the yeast was inoculated into the effluent and incubated for 15 days. Saccharomyces cerevisiae shows the highest percentage reduction of 52.5, 52.5 and 58.7% for BOD, COD and nitrate respectively of the pharmaceutical effluent and closely followed by the consortium which has 44.5, 44.5 and 72.0% for BOD, COD and nitrate reduction, respectively. The least percentage reduction was displayed by Torulaspora delbrueckii with 38.3, 38.3 and 79.7%. The study revealed that Saccharomyces cerevisiae isolated from spoilt water melon could be used in the biological treatment of pharmaceutical effluent. © 2015 Academic Journals Inc.


Sunday R.M.,National Biotechnology Development Agency | Ilesanmi O.R.,Obafemi Awolowo University | Obuotor E.M.,Obafemi Awolowo University
Research Journal of Medicinal Plant | Year: 2016

This study was carried out to evaluate the potential anti-diabetic effect of Anthocleista vogelii ethanolic root extract in alloxan-induced diabetic rats. Albino rats of both sexes were randomly divided into five groups with five rats each. Group 1 (control; 10 mL kg-1 distilled water), group 2-4 (100, 200 and 400 mg kg-1 A. vogelii ethanolic root extract) and group 5 (5 mg kg-1 glibenclamide). Diabetes was induced physiologically using 10 g kg-1 glucose p.o. and chemically using 150 mg kg-1 alloxan i.p. Fasting blood glucose levels of the diabetic rats were determined at intervals of 30, 60, 120 and 240 min in glucose loaded rats and on days 4, 7, 10 and 14 in alloxan-induced diabetic rats. After two weeks, the levels of serum cholesterol, triglyceride, high density lipoprotein, low density lipoprotein, alanine aminotransferase, aspartate amino transferase and creatinine of all the groups were analyzed. The LD50 of A. vogelii ethanolic root extract was≥5000 mg kg-1 (p.o.). The extract exerted a significant (p<0.05) reduction in Fasting blood glucose levels, serum cholesterol, triglyceride, low density lipoprotein, creatinine, alanine aminotransferase and aspartate aminotransferase levels and an increase in serum high density lipoprotein levels when compared to the control. The extract also elicited a significant decrease in body weight, food and water intake in diabetic treated rats. The results show that A. vogelii ethanolic root extract have anti-diabetic and anti-hyperlipideamic effect when administered for 14 days in alloxan-induced diabetic rats. © 2016 Academic Journals Inc.


Olofinsae S.A.,University of East London | Ibeh B.O.,National Biotechnology Development Agency | Ahluwalia J.,University of East London
Asian Pacific Journal of Tropical Medicine | Year: 2014

Objective: To investigate the effect of phorbol 12-myristate 13-acetate (PMA) and formyl-methionyl-leucyl-phenylalanine (FMLP) on oxygen consumption of differentiated and non-differentiated immune cell lines by retinoic acid and calcitriol treatment which might be useful in subsequent elicitation of immunological action during immunosuppressive states. Methods: PMA and FMLP were used to artificially stimulate reactive oxygen production in cultured promonocytic U937 cell line. Paralleled samples of the cultured cells were separately prepared with calcitriol (1, 25- dihydroxyvitamin D3) and retinoic acid followed by a 72-hour incubation period. The rate of respiratory burst was measured using the Clark oxygen electrode. Results: The average increase in cell concentrations per mL observed was significantly higher in retinoic acid-treated cells (9×106 cells/mL) when compared with calcitriol-treated samples (4×106 cells/mL). There was a marked increase in oxygen consumption of the calcitriol-treated cell lines against the retinoic acid-treated ones. Exposure of differentiated U937 cells to PMA and FMLP increased significantly (P<0.05) in their oxygen consumption when compared with the control. PMA calcitriol-treated cells resulted in 55% oxygen consumption more than the control while FMLP oxygen consumption increased 78% by comparison with the control. Conclusions: The result demonstrated that calcitriol may serve as a physiological promoter of normal differentiation of precursor cells which may exert an immunological action. This effect could elicit a marker potential and increase immune cell activity of the host especially in immunosuppressed diseased states. © 2014 Hainan Medical College.


Yusuf K.,National Biotechnology Development Agency | Amasiora V.,National Biotechnology Development Agency | Ashanu E.,National Biotechnology Development Agency
African Journal of Biotechnology | Year: 2010

The last century has been largely driven by wealth of natural resources; however, more than ever before, knowledge, skills and intellectualism are now the driving force of current global wealth creation. The demonstration of this fact can be found with nations that have advanced their science and technology system and have adopted new technologies. Knowledge of space science, information technology and biotechnology in particular are been explored to narrow the divide between 'have' and 'have not' in these communities. The application of biotechnology is not new, it has been employed for centuries in the production of fermented foods such as gari, bread, beer, yoghurt, cheese and beverages such as wine. Modern advances in biotechnology hold great promise for addressing key challenges in agriculture, human health and the environment. Biotechnology, as with any new technology has its advantages and limitations; the application of modern biotechnology has highlighted its positive impact on agriculture, human health and the environment through increased crop yields, the reduced use of pesticides and herbicides, production of nutritionally enhanced foods and affordable vaccines. It is indeed an essential panacea to the pervasive poverty and food security problem in Nigeria. The debate about biotechnology continues because of, politics, trade and ethical issues that have been raised in the public domain with little distinction being made between biotechnology as a tool and genetically modified (GM) crops and foods as products, leading to the intense controversy about the perceived risks to human health and environment. This highlights the importance of having biosafety regulations in place and ensuring that there is adequate in-country capacity so that all the necessary precautions are adhered to. The debate must shift to how this technology can be adopted and deployed to benefit the nation and its citizens in such a way that the ecosystem is not threatened. © 2010 Academic Journals.


PubMed | University of East London and National Biotechnology Development Agency
Type: | Journal: Asian Pacific journal of tropical medicine | Year: 2014

To investigate the effect of phorbol 12-myristate 13-acetate (PMA) and formyl-methionyl-leucyl-phenylalanine (FMLP) on oxygen consumption of differentiated and non-differentiated immune cell lines by retinoic acid and calcitriol treatment which might be useful in subsequent elicitation of immunological action during immunosuppressive states.PMA and FMLP were used to artificially stimulate reactive oxygen production in cultured promonocytic U937 cell line. Paralleled samples of the cultured cells were separately prepared with calcitriol (1, 25- dihydroxyvitamin D3) and retinoic acid followed by a 72-hour incubation period. The rate of respiratory burst was measured using the Clark oxygen electrode.The average increase in cell concentrations per mL observed was significantly higher in retinoic acid-treated cells (910(6) cells/mL) when compared with calcitriol-treated samples (410(6) cells/mL). There was a marked increase in oxygen consumption of the calcitriol-treated cell lines against the retinoic acid-treated ones. Exposure of differentiated U937 cells to PMA and FMLP increased significantly (P<0.05) in their oxygen consumption when compared with the control. PMA calcitriol-treated cells resulted in 55% oxygen consumption more than the control while FMLP oxygen consumption increased 78% by comparison with the control.The result demonstrated that calcitriol may serve as a physiological promoter of normal differentiation of precursor cells which may exert an immunological action. This effect could elicit a marker potential and increase immune cell activity of the host especially in immunosuppressed diseased states.

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