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Hao L.M.,China Institute of Medical Equipment | Hao L.M.,National Bioprotection Engineering Center | Jia H.Q.,China Institute of Medical Equipment | Jia H.Q.,National Bioprotection Engineering Center
Advanced Materials Research | Year: 2012

In the research of bio-disinfector, it needs neutralizers to eliminate the antibacterial activity of disinfector during the disinfection experiment. But there was no report about the appropriate neutralizer of antibacterial peptides. In this paper, we study the neutralizer suitable for ε-polylysine by using the orthogonal design and optimize the combination of neutralizers. Then we performed the checkup experiment of neutralizer according to the method listed in Disinfection Technology Standard. Finally we successfully obtain the right neutralizer of ε-polylysine which is the combination of 0.5 M BSA and 0.5 M K 2HPO 4, This can provide a convenience for researchers to evaluate the disinfection effect of disinfector whose main component is ε-polylysine. © (2012) Trans Tech Publications, Switzerland. Source


Hao L.,China Institute of Medical Equipment | Hao L.,National Bioprotection Engineering Center | Wang Z.,China Institute of Medical Equipment | Wang Z.,National Bioprotection Engineering Center | And 8 more authors.
Journal Wuhan University of Technology, Materials Science Edition | Year: 2011

A novel antibacterial material (L-PET) was prepared by immobilizing η-polylysine on polyethylene terephthalate (PET) nonwoven fabrics. Surface modifications of the fabric were performed by using a chemical modifi cation procedure where carboxyl groups were prepared on the PET surface, a coupling agent was grafted, and the η-polylysine was immobilized. Scanning electron microscopy (SEM) was used to analyze the surface morphology of the fabrics, while the toluidine blue method and X-ray photoelectron spectroscopy (XPS) were used to evaluate the grafting densities. The antibacterial activities of the L-PET were investigated by using the shaking-fl ask method. The electron micrographs showed that the surface of the blank PET and the modified fabrics did not change. The results of XPS analysis confirmed that η-polylysine was successfully grafted onto the surface of PET. The results of the antibacterial experiments showed that L-PET fabrics had excellent antibacterial activity against Escherichia coli and Staphylococcus aureus, and that L-PET fabrics were stable in storage for at least two years. © 2011 Wuhan University of Technology and Springer Verlag Berlin Heidelberg. Source

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