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Wang R.,National Bio Protection Engineering Center | Wang R.,China Institute of Medical Equipment | Wang Z.,National Bio Protection Engineering Center | Wang Z.,China Institute of Medical Equipment | And 6 more authors.
RSC Advances | Year: 2015

Nanohybrids from waterborne polyurethane (WPU), poly(vinyl alcohol) (PVA) and silver nanoparticles (AgNPs) with ultrasmall sizes (5.1 ± 0.6 nm) are facilely obtained by directly one-step dual-spinneret electrospinning fabrication from water. SEM and TEM images indicated the nanofibre morphology as designed. Moreover, the elemental distribution mapping indicated the good mixture of WPU/PVA and PVA/AgNPs nanofibers in the mats via such dual-spinneret technique. Also the thermal stability and biocidal activity of the hybrid nanofibers were greatly enhanced by the addition of WPU component and incorporation of Ag nanoparticles without increase of cytotoxicity. © The Royal Society of Chemistry 2015.


Jia H.Q.,National Bio protection Engineering Center | Li Y.J.,National Bio protection Engineering Center | Sun B.,Academy of Military Medical science | Zhao S.Q.,Academy of Military Medical science | And 5 more authors.
Biomedical and Environmental Sciences | Year: 2013

Objective To evaluate the performance of vaporized hydrogen peroxide (VHP) for the bio-decontamination of the high efficiency particulate air (HEPA) filter unit. Methods Self-made or commercially available bioindicators were placed at designated locations in the HEPA filter unit under VHP fumigation. The spores on coupons were then extracted by 0.5 h submergence in eluent followed by 200- time violent knocks. Results Due to the presence of HEPA filter in the box, spore recovery from coupons placed at the bottom of the filter downstream was significantly higher than that from coupons placed at the other locations. The gap of decontamination efficiency between the top and the bottom of the filter downstream became narrower with the exposure time extended. The decontamination efficiency of the bottom of the filter downstream only improved gently with the injection rate of H2O2 increased and the decontamination efficiency decreased instead when the injection rate exceeded 2.5 g/min. The commercially available bioindicators were competent to indicate the disinfection efficiency of VHP for the HEPA filter unit. Conclusion The HEPA filter unit is more difficult than common enclosure to decontaminate using VHP fumigation. Complete decontamination can be achieved by extending fumigation time. VHP fumigation can be applied for in-situ biodecontamination of the HEPA filter unit as an alternative method to formaldehyde fumigation. © 2013 The Editorial Board of Biomedical and Environmental Sciences.


PubMed | National Bio Protection Engineering Center, China Institute of Medical Equipment and Shanghai University
Type: | Journal: International journal of nanomedicine | Year: 2014

Submicrometer-scale poly(vinyl alcohol) (PVA) nanofibrous mats loaded with aligned and narrowly dispersed silver nanoparticles (AgNPs) are obtained via the electrospinning process from pure water. This facile and green procedure did not need any other chemicals or organic solvents. The doped AgNPs are narrowly distributed, 4.30.7 nm and their contents on the nanofabric mats can be easily tuned via in situ ultraviolet light irradiation or under preheating conditions, but with different particle sizes and size distributions. The morphology, loading concentrations, and dispersities of AgNPs embedded within PVA nanofiber mats are characterized by transmission electron microscopy, scanning electron microscopy, energy dispersive X-ray spectroscopy, ultraviolet-visible spectra, X-ray photoelectron spectroscopy, and X-ray diffraction, respectively. Moreover, the biocidal activities and cytotoxicity of the electrospun nanofiber mats are determined by zone of inhibition, dynamic shaking method, and cell counting kit (CCK)-8 assay tests.


Chen F.,Tianjin University | Du Y.H.,National Bio Protection Engineering Center | Cheng Z.,National Bio Protection Engineering Center | Xu K.X.,Tianjin University | Wu T.H.,National Bio Protection Engineering Center
Advanced Materials Research | Year: 2013

A new type detecting device was developed according to the demand of the liquid single particle size analysis. Based on side scatter detection technology, the device used a semiconductor laser as light source. By introduction of the hydrodynamics, sample particles were focused sequentially through the measuring beam. The lateral scattering lights were received by high-sensitivity avalanche photodiode. So the particles' size can be achieved by analyzing the scattered light pulse signal magnitude. Experiment results showed that the device can detect 0.35μm particle effectively, and its particle size detection limit is superior to the existing liquid particle detection products in the market. In addition, the hydrodynamics focusing technology contributed to more prominently distinguish rate. The device has broad application prospects in the pharmaceutical, medical and polymer industries. © (2013) Trans Tech Publications, Switzerland.


Che W.,China Institute of Medical Equipment | Liu C.,China Institute of Medical Equipment | Liu C.,National Bio protection Engineering Center | Yang K.,China Institute of Medical Equipment | And 3 more authors.
Fuhe Cailiao Xuebao/Acta Materiae Compositae Sinica | Year: 2016

Fluorescent carbon dots are one of the most popular carbon nanomaterials after carbon nanotube, nano-diamond and graphene. Owing to its advantages of excellent photoluminescence, small size features, good biocompatibility, low cytotoxicity and ease to achieve surface functionalization compared with conventional semiconductor quantum dots, carbon dots have great application value in the field of environmental detection, biochemical sensing, drug carriers, photocatalytic and electrocatalytic technology and other areas. The progress in composition methods, structure, property and application research of carbon dots are summarized, the bottleneck problems of restricting carbon dots application development are pointed out and main further research direction is prospected. © 2016, BUAA Culture Media Group Ltd. All right reserved.


Han J.,China Institute of Medical Equipment | Wang Z.,National Bio Protection Engineering Center | Wu L.,China Institute of Medical Equipment | Wu C.,China Institute of Medical Equipment | Zhang W.,China Institute of Medical Equipment
Advanced Materials Research | Year: 2012

In this paper, a small medical waste pyrolysis furnance, which could disposal 1t wastes, is designed for the safe disposal of medical wastes, by in-depth research of pyrolysis, detailed calculation analysis, and considering the experimental results and other factors in general. The flue gas treatment system of the small medical waste pyrolysis furnance is designed applying 3T principle, and the dioxin's later synthesis is controlled, so the dioxin emissions problem is also resolved. The results show that the small furnance has reasonable configuration, works reliably, and it could self-support freely, its emissions meet the national standards for the dioxin emissions 0.213 ng/m 3. © (2012) Trans Tech Publications, Switzerland.


Zhang Z.,National Bio Protection Engineering Center | Yi Y.,National Bio Protection Engineering Center | Zhao M.,National Bio Protection Engineering Center | Wang W.,National Bio Protection Engineering Center | Qi J.,National Bio Protection Engineering Center
HVAC and R Research | Year: 2014

High-efficiency air filtration is an important means of preventing harmful aerosol overflow in biosafety laboratories. For convenience in the in situ scanning leak test of a high-efficiency particulate air (HEPA) filter to ensure the filter's intactness, a cylindrical blunt sampling probe is developed. The sampling inlet is of the slot type and the inlet's length equal the width of the HEPA filter. Thus, the entire downstream face of the HEPA filter may be scanned. To evaluate the blunt sampling probe's ability to detect leaks, the scanning leak test performed on a HEPA filter unit was compared with a test using the thin-walled rectangular sampling probe that is recommended by relevant standards. Based on the comparison, the leak penetration determined using the blunt sampling probe was slightly lower than that determined using the reference sampling probe. Additionally, differences were observed according to the location that was tested. However, the blunt sampling probe could detect leaks through the comprehensive analysis of the local penetration of most-penetrating particle size (MPPS) and non-MPPS particles. © 2014 Taylor and Francis Group, LLC.


Lin S.,National Bio protection Engineering Center | Lin S.,China Institute of Medical Equipment | Wu J.-H.,China Institute of Medical Equipment | Jia H.-Q.,China Institute of Medical Equipment | And 4 more authors.
RSC Advances | Year: 2013

One kind of polymeric quaternary ammonium salts derived from dimethylaminoethyl methacrylate are synthesised and their antibacterial efficacy is evaluated, indicating that the antibacterial efficacy is greatly enhanced after polymerisation and the mechanism of this effect is clarified by zeta potential tests, SEM and protein leakage measurements. © The Royal Society of Chemistry 2013.


PubMed | National Bio protection Engineering Center
Type: Journal Article | Journal: Biomedical and environmental sciences : BES | Year: 2013

To evaluate the performance of vaporized hydrogen peroxide (VHP) for the bio-decontamination of the high efficiency particulate air (HEPA) filter unit.Self-made or commercially available bioindicators were placed at designated locations in the HEPA filter unit under VHP fumigation. The spores on coupons were then extracted by 0.5 h submergence in eluent followed by 200- time violent knocks.Due to the presence of HEPA filter in the box, spore recovery from coupons placed at the bottom of the filter downstream was significantly higher than that from coupons placed at the other locations. The gap of decontamination efficiency between the top and the bottom of the filter downstream became narrower with the exposure time extended. The decontamination efficiency of the bottom of the filter downstream only improved gently with the injection rate of H2O2 increased and the decontamination efficiency decreased instead when the injection rate exceeded 2.5 g/min. The commercially available bioindicators were competent to indicate the disinfection efficiency of VHP for the HEPA filter unit.This assay developed can detect all 16 -lactams demanded by the European Union (EU). The whole procedure takes only 45 min and can detect 42 samples and the standards with duplicate analysis.


Wang T.,China Institute of Medical Equipment | Wang T.,National Bio Protection Engineering Center | Wu J.,China Institute of Medical Equipment | Wu J.,National Bio Protection Engineering Center | And 8 more authors.
Applied and Environmental Microbiology | Year: 2016

Bacillus subtilis subsp. niger spore and Staphylococcus albus are typical biological indicators for the inactivation of airborne pathogens. The present study characterized and compared the behaviors of B. subtilis subsp. niger spores and S. albus in regard to inactivation by chlorine dioxide (ClO2) gas under different gas concentrations and relative humidity (RH) conditions. The inactivation kinetics under different ClO2 gas concentrations (1 to 5 mg/liter) were determined by first-order and Weibull models. A new model (the Weibull-H model) was established to reveal the inactivation tendency and kinetics for ClO2 gas under different RH conditions (30 to 90%). The results showed that both the gas concentration and RH were significantly (P < 0.05) and positively correlated with the inactivation of the two chosen indicators. There was a rapid improvement in the inactivation efficiency under high RH (> 70%). Compared with the first-order model, the Weibull and Weibull-H models demonstrated a better fit for the experimental data, indicating nonlinear inactivation behaviors of the vegetative bacteria and spores following exposure to ClO2 gas. The times to achieve a six-log reduction of B. subtilis subsp. niger spore and S. albus were calculated based on the established models. Clarifying the kinetics of inactivation of B. subtilis subsp. niger spores and S. albus by ClO2 gas will allow the development of ClO2 gas treatments that provide an effective disinfection method. © 2016, American Society for Microbiology.

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