National Beef Cattle Improvement Center

Yangling, Taiwan

National Beef Cattle Improvement Center

Yangling, Taiwan
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Wei S.,Northwest University, China | Wei S.,Washington State University | Zan L.S.,Northwest University, China | Zan L.S.,National Beef Cattle Improvement Center | And 7 more authors.
Genetics and Molecular Research | Year: 2013

Fatty acid binding protein 4 (FABP4) is an important adipocyte gene, with roles in fatty acid transport and fat deposition in animals as well as human metabolic syndrome. However, little is known about the functional regulation of FABP4 at the cellular level in bovine. We designed and selected an effective shRNA (small hairpin RNA) against bovine FABP4, constructed a corresponding adenovirus (AD-FABP4), and then detected its influence on mRNA expression of four differentiation-related genes (PPARγ, CEBPA, CEBPB, and SREBF1) and three lipid metabolism-related genes (ADIPOQ, LEP and LEPR) of adipocytes. The FABP4 mRNA content, derived from bovine adipocytes, decreased by 41% (P < 0.01) after 24 h and 66% (P < 0.01) after 72 h of AD-FABP4 infection. However, lower mRNA content of FABP4 did not significantly alter levels of differentiation-related gene expression at 24 h following AD-FABP4 treatment of bovine-derived preadipocytes (P = 0.54, 0.78, 0.89, and 0.94, respectively). Meanwhile, knocking down (partially silencing) FABP4 significantly decreased ADIPOQ (P < 0.05) and LEP (P < 0.01) gene expression after 24 h of AD-FABP4 treatment, decreased ADIPOQ (P < 0.01) and LEP (P < 0.01) gene expression, but increased LEPR mRNA expression (P < 0.01) after a 72-h treatment of bovine preadipocytes. We conclude that FABP4 plays a role in fat deposition and metabolic syndrome by regulating lipid metabolism-related genes (such as ADIPOQ, LEP and LEPR), without affecting the ability of preadipocytes to differentiate into adipocytes. © FUNPEC-RP.


Wei S.,Northwest University, China | Zan L.,Northwest University, China | Zan L.,National Beef Cattle Improvement Center | Ujan J.A.,Northwest University, China | And 3 more authors.
African Journal of Biotechnology | Year: 2011

In this study, genetic variation of the fat mass and obesity-associated gene (FTO) was detected by PCRSSCP and DNA sequencing in 618 individuals from five Chinese indigenous cattle breeds, and their genetic effects on meat quality traits were evaluated. The results showed that a novel single nucleotide polymorphism C1071T was detected in exon 5 and the allelic frequencies for the C and T alleles of the five breeds were 0.666/0.334, 0.583/0.417, 0.631/0.369, 0.653/0.347 and 0.689/0.311, respectively. Animals with CT genotype had higher mean values for backfat thickness than those with CC or TT genotypes (P < 0.01). Individuals with CC or CT genotypes had higher longissimus muscle area than those with TT genotype (P < 0.05). The FTO gene may be a candidate gene for identifying differences in meat quality traits and therefore, could be applied to marker-assisted selection of native Chinese cattle breeds. © 2011 Academic Journals.


Tian W.Q.,Northwest University, China | Wang H.C.,Northwest University, China | Song F.B.,Northwest University, China | Zan L.S.,Northwest University, China | And 6 more authors.
Genetics and Molecular Research | Year: 2011

Qinchuan is a red or yellow draft and beef breed in China. In order to identify a predictor of carcass traits on the basis of associations between carcass traits and gene polymorphism, variation in the bovine chemerin gene was investigated using PCR-single-strand conformational polymorphism and DNA sequencing. An SNP of A868G located in exon 2 of the Bos taurus chemerin gene was detected in 716 samples of six breeds (Jiaxian red, Luxi, Nan yang, Qinchuan, Simmental and Luxi crossbred steers, and Xia’nan), all in China, and three genotypes (AA, AG and GG) were found. Based on the χ2 test, the AA/AG/GG genotype frequencies of all six breeds were found to be in Hardy-Weinberg equilibrium. A possible association of A868G with some carcass traits was investigated in 106 Qinchuan cattle. Animals with the AG genotype were found to have significantly lower mean loin eye area and meat tenderness compared to those with the AA and GG genotypes. However, there was no significant association between any individual haplotype and backfat thickness, water holding capacity or marbling score. We suggest that A868G could be used as a molecular marker in marker-assisted selection for carcass traits. © FUNPEC-RP.


Ujan J.A.,Northwest University, China | Zan L.S.,Northwest University, China | Zan L.S.,National Beef Cattle Improvement Center | Ujan S.A.,Shah Abdul Latif University | And 3 more authors.
Genetics and Molecular Research | Year: 2011

Qualitative trait loci (QTL) for growth and meat quality traits in cattle (Bos taurus) have been previously mapped to three chromosome regions, 0 to 30, 55 to 70, and 70 to 80 cM on chromosome 5. We evaluated the allele frequencies and gene-specific single nucleotide polymorphisms (SNPs) of bovine myogenic factor 5 (MyF-5) in the QTL regions and their associations with live weight and meat characteristics in indigenous Chinese cattle breeds. PCR-SSCP methodology showed a T>A mutation at 526 bp. Least square analysis revealed a significant association of this SNP with backfat thickness and meat tenderness (P < 0.05), while no significant association was found with live weight, loin eye height, loin eye area, rib area, or water holding capacity. Allele frequencies of MyF-5-A/B in the five breeds were 0.760/0.239, 0.752/0.247, 0.629/0.370, 0.715/0.284, and 0.750/0.250, for JiaXian red, Luxi, Nanyang, Qinchuan, and XiaNan crossbreed, respectively. The genotype distributions for these alleles in two of the Chinese cattle breeds (Luxi and Qinchuan) were not in Hardy-Weinberg equilibrium (P > 0.05); while those for the other three breeds (JiaXian red, Nanyang, and XiaNan) were in agreement with Hardy-Weinberg equilibrium (P > 0.05). The genotypic frequencies among all five cattle breeds showed moderate diversity (0.25 < polymorphism information content < 0.5). Based on our findings, we suggest that the MyF-5 gene influences back fat thickness and meat tenderness in Chinese Bos taurus. This SNP could be useful for markerassisted selection for meat quality traits in these cattle. © FUNPEC-RP www.funpecrp.com.br.


Wang H.B.,Northwest University, China | Wang H.B.,National Beef Cattle Improvement Center | Zan L.S.,Northwest University, China | Zan L.S.,National Beef Cattle Improvement Center | Zhang Y.Y.,Northwest University, China
Animal | Year: 2014

Of all the mammals of the world, the yak lives at the highest altitude area of more than 3000 m. Comparison between yak and cattle of the low-altitude areas will be informative in studying animal adaptation to higher altitudes. To investigate the molecular mechanism involved in meat quality differences between the two Chinese special varieties Qinghai yak and Qinchuan cattle, 12 chemical-physical characteristics of the longissimus dorsi muscle related to meat quality were compared at the age of 36 months, and the gene expression profiles were constructed by utilizing the bovine genome array. Significant analysis of microarrays was used to identify the differentially expressed genes. Gene ontology and pathway analysis were performed by a free Web-based Molecular Annotation System 2.0. The results reveal ~11 000 probes representing about 10 000 genes that were detected in both the Qinghai yak and Qinchuan cattle. A total of 1922 genes were shown to be differentially expressed, 633 probes were upregulated and 1259 probes were downregulated in the muscle tissue of Qinghai yak that were mainly involved in ubiquitin-mediated proteolysis, muscle growth regulation, glucose metabolism, immune response and so on. Quantitative real-time PCR (qRT-PCR) was performed to validate some differentially expressed genes identified by microarray. Further analysis implied that animals living at a high altitude may supply energy by more active protein catabolism and glycolysis compared with those living in the plain areas. Our results establish the groundwork for further studies on yaks' meat quality and will be beneficial in improving the yaks' breeding by molecular biotechnology. Copyright © The Animal Consortium 2013.


Fan Y.Y.,Northwest University, China | Zan L.S.,Northwest University, China | Zan L.S.,National Beef Cattle Improvement Center | Fu C.Z.,Northwest University, China | And 5 more authors.
Molecular Biology Reports | Year: 2011

The peroxisome proliferator-activated receptor γ (PPARγ) is a nuclear hormone receptor that regulates adipogenesis and many other biological processes. In the present study, we carried out PCR-SSCP and DNA sequencing analyses to examine SNPs in coding region of the PPARγ gene. A total of 660 individuals from five Chinese cattle breeds were genotyped. We identified three SNPs and their associations with meat quality traits were analyzed in 108 Qinchuan cattle. Two missense mutations and one synonymous mutation were found: 200 A > G (genotypes AA, AB and BB) resulting in D7G change, the silent substitution 42895 C > T (genotypes JJ and JI) and 72472 G > T (genotypes CC, DC and DD) producing Q448H change, respectively. The frequencies of PPARγ-A allele were 0.86, 0.83, 0.80, 0.72 and 0.87 for Qinchuan, Nanyang, Jiaxian, Luxi and Xianan populations, respectively. The frequencies of PPARγ-J allele varied from 0.87 to 0.96 in the five populations. In the 72472 G > T locus, the frequencies of PPARγ-C allele were higher than PPARγ-D allele in the five populations, and ranged from 0.58 to 0.82. Least squares analysis revealed that in 42895 C > T locus, there was a significant effect on tenderness in 18-20 months Qinchuan cattle (P < 0.01), and in the 72472 G > T locus, animals with the genotype DC had lower mean values than these with genotype CC (P < 0.01) for back fat thickness in 18-20 months, and animals with the genotype DD had lower mean values than these with genotypes CC and DC (P < 0.01) for water holding capacity in 21-24 months (P < 0.01). The SNPs we have identified may contribute to establishing a more efficient selection program for improving of genetic characteristics in indigenous Chinese cattle © 2010 Springer Science+Business Media B.V.


Zhang Y.,Northwest University, China | Zan L.,Northwest University, China | Zan L.,National Beef Cattle Improvement Center | Wang H.,Northwest University, China | Wang H.,National Beef Cattle Improvement Center
Molecular Biology Reports | Year: 2011

In order to screen candidate genes related to tenderness trait in Qinchuan cattle, we investigated the gene expression profile of Longuissimus dorsi muscle (LDM) tissue and screened differentially expressed genes in LDM from both male and female Qinchuan cattle at 36 months of age utilising Bovine Genome Array. Significance Analysis of Microarrays (SAM) was used to identify the differentially expressed genes, Go (Gene Ontology) and pathways analysis were conducted on which by a free web-based Molecular Annotation System 2.0 (MAS 2.0). Approximately 11,000 probe sets representing 10,000 genes were detected in LDM of 36 month old Qinchuan cattle. After SAM analysis of the microarray data, 598 genes were shown to be differentially expressed. These genes were predominantly involved in cell adhesion, collagen fibril organization and synthesis, immune responses and cell-matrix adhesion. They included cell adhesion molecules (CAMs) and ECM-receptor interaction molecules. Real-time PCR was performed to validate nine of the differentially expressed genes identified by microarray. The results suggest that at the transcriptional level the residual hardness caused by connective tissues, stroma protein and muscle tissues could mainly result in tenderness differences between male and female Qinchuan cattle. © 2010 Springer Science+Business Media B.V.


Gao L.,Northwest University, China | Ujan J.A.,Northwest University, China | Zan L.,Northwest University, China | Zan L.,National Beef Cattle Improvement Center | And 2 more authors.
African Journal of Biotechnology | Year: 2011

Searching for candidate gene polymorphisms and their relationship with meat quality traits is an important issue for Bos taurus industry. In this study, we evaluated polymorphism of resistin (RETN) gene involved in energy metabolism. Using the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technology, a novel missense substitution single-nucleotide polymorphism (SNP) g.2528T>C was identified in the exon 2 region of the RETN gene. Allele frequencies, gene heterozygosity, effective allele number and polymorphism information content of the bovine RETN SNP in six populations were investigated and calculated by the χ 2 test. The distribution of the polymorphism from the studied six B. taurus breeds was not uniform. Our results suggested that Qinchuan, Luxi and Luxi × Simmental were not in Hardy-Weinberg equilibrium. Moreover, the polymorphism and its association with meat quality traits were analyzed in 369 Qinchuan and 73 Nanyang individuals. The analyzed SNP in the RETN revealed a significant association with marbling and intramuscular fat (p < 0.05). We observed that TT genotype was associated with a heightened marbling score when compared with the TC and CC in Qinchuan and Nanyang breeds. In Qinchuan breed, we also found that TT genotype was associated with an increased intramuscular fat content when compared with the TC. Such associations were not observed on backfat thickness, loin-muscle area and loin-muscle depth. This study showed that polymorphism of the RETN gene is potentially associated with B. taurus meat quality traits. © 2011 Academic Journals.


Liu H.,Northwest University, China | Tian W.,Northwest University, China | Zan L.,Northwest University, China | Zan L.,National Beef Cattle Improvement Center | And 3 more authors.
Molecular Biology Reports | Year: 2010

MC4R belongs to a seven-transmembrane G-protein-coupled receptor which may regulate body composition and insulin action. Many mutations in the MC4R gene are associated with obesity, energy expenditure and serum triglyceride levels in human and animals. Six mutations in the MC4R gene were identified in our study (-293C>G, -193A>T, -192T>G, -129A>G, -84T>C and 1,069C>G). The -129A>G was significantly associated with live weight (LW) (P < 0.05), Cattle with the genotypes AG and GG had higher LW than genotype AA. The 1,069C>G was significantly associated with LW, carcass weight (CW), backfat thickness and marbling score (MS). Cattle with the genotype GG had higher LW, CW and MS than genotype CC; Cattle with the genotypes GG and CG had higher MS than CC. The results suggested that -129A>G and 1,069C>G SNP of the MC4R gene may be useful as a genetic marker for carcass and meat quality traits in Qinchuan cattle. © 2009 Springer Science+Business Media B.V.


Zhang Y.-Y.,Shaanxi University of Science and Technology | Zan L.-S.,Shaanxi University of Science and Technology | Zan L.-S.,National Beef Cattle Improvement Center | Wang H.-B.,Shaanxi University of Science and Technology | And 5 more authors.
African Journal of Biotechnology | Year: 2010

A total of 18 Qinchuan cattle, six intact males (IM), six castrated males (CM) and six females (FM), were used to investigate the effect of sex on the physicochemical characteristics (PCC) and fatty acid (FA) composition of the Longissimus dorsi muscle (LDM). Obvious sex differences were found in the PCC of LDM: the IM group had higher shear value, pH, drip and cooking losses, and contents of ash and hydroxyproline (Hyp) than the CM and FM groups, as well as lower ether extract content and lightness. Both the IM and CM groups had lower water content and higher protein content than the FM group. Sex differences were also observed in contents of C14:0, C14:1, C18:1, saturated fatty acids (SFA), monounsaturated fatty acids (MUFA) and unsaturated fatty acids (UFA) between the IM and both the CM and FM groups but not between the CM and FM groups. The results indicated that sex is an important source of differences in meat quality of Qinchuan cattle because the castration and the meat characteristics of the CM group were more similar to the FM than the IM group. © 2010 Academic Journals.

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