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Gao L.,Northwest University, China | Ujan J.A.,Northwest University, China | Zan L.,Northwest University, China | Zan L.,National Beef Cattle Improvement Center | And 2 more authors.
African Journal of Biotechnology | Year: 2011

Searching for candidate gene polymorphisms and their relationship with meat quality traits is an important issue for Bos taurus industry. In this study, we evaluated polymorphism of resistin (RETN) gene involved in energy metabolism. Using the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technology, a novel missense substitution single-nucleotide polymorphism (SNP) g.2528T>C was identified in the exon 2 region of the RETN gene. Allele frequencies, gene heterozygosity, effective allele number and polymorphism information content of the bovine RETN SNP in six populations were investigated and calculated by the χ 2 test. The distribution of the polymorphism from the studied six B. taurus breeds was not uniform. Our results suggested that Qinchuan, Luxi and Luxi × Simmental were not in Hardy-Weinberg equilibrium. Moreover, the polymorphism and its association with meat quality traits were analyzed in 369 Qinchuan and 73 Nanyang individuals. The analyzed SNP in the RETN revealed a significant association with marbling and intramuscular fat (p < 0.05). We observed that TT genotype was associated with a heightened marbling score when compared with the TC and CC in Qinchuan and Nanyang breeds. In Qinchuan breed, we also found that TT genotype was associated with an increased intramuscular fat content when compared with the TC. Such associations were not observed on backfat thickness, loin-muscle area and loin-muscle depth. This study showed that polymorphism of the RETN gene is potentially associated with B. taurus meat quality traits. © 2011 Academic Journals.

Wei S.,Northwest University, China | Wei S.,Washington State University | Zan L.S.,Northwest University, China | Zan L.S.,National Beef Cattle Improvement Center | And 7 more authors.
Genetics and Molecular Research | Year: 2013

Fatty acid binding protein 4 (FABP4) is an important adipocyte gene, with roles in fatty acid transport and fat deposition in animals as well as human metabolic syndrome. However, little is known about the functional regulation of FABP4 at the cellular level in bovine. We designed and selected an effective shRNA (small hairpin RNA) against bovine FABP4, constructed a corresponding adenovirus (AD-FABP4), and then detected its influence on mRNA expression of four differentiation-related genes (PPARγ, CEBPA, CEBPB, and SREBF1) and three lipid metabolism-related genes (ADIPOQ, LEP and LEPR) of adipocytes. The FABP4 mRNA content, derived from bovine adipocytes, decreased by 41% (P < 0.01) after 24 h and 66% (P < 0.01) after 72 h of AD-FABP4 infection. However, lower mRNA content of FABP4 did not significantly alter levels of differentiation-related gene expression at 24 h following AD-FABP4 treatment of bovine-derived preadipocytes (P = 0.54, 0.78, 0.89, and 0.94, respectively). Meanwhile, knocking down (partially silencing) FABP4 significantly decreased ADIPOQ (P < 0.05) and LEP (P < 0.01) gene expression after 24 h of AD-FABP4 treatment, decreased ADIPOQ (P < 0.01) and LEP (P < 0.01) gene expression, but increased LEPR mRNA expression (P < 0.01) after a 72-h treatment of bovine preadipocytes. We conclude that FABP4 plays a role in fat deposition and metabolic syndrome by regulating lipid metabolism-related genes (such as ADIPOQ, LEP and LEPR), without affecting the ability of preadipocytes to differentiate into adipocytes. © FUNPEC-RP.

Wei S.,Northwest University, China | Zan L.,Northwest University, China | Zan L.,National Beef Cattle Improvement Center | Ujan J.A.,Northwest University, China | And 3 more authors.
African Journal of Biotechnology | Year: 2011

In this study, genetic variation of the fat mass and obesity-associated gene (FTO) was detected by PCRSSCP and DNA sequencing in 618 individuals from five Chinese indigenous cattle breeds, and their genetic effects on meat quality traits were evaluated. The results showed that a novel single nucleotide polymorphism C1071T was detected in exon 5 and the allelic frequencies for the C and T alleles of the five breeds were 0.666/0.334, 0.583/0.417, 0.631/0.369, 0.653/0.347 and 0.689/0.311, respectively. Animals with CT genotype had higher mean values for backfat thickness than those with CC or TT genotypes (P < 0.01). Individuals with CC or CT genotypes had higher longissimus muscle area than those with TT genotype (P < 0.05). The FTO gene may be a candidate gene for identifying differences in meat quality traits and therefore, could be applied to marker-assisted selection of native Chinese cattle breeds. © 2011 Academic Journals.

Ujan J.A.,Northwest University, China | Zan L.S.,Northwest University, China | Zan L.S.,National Beef Cattle Improvement Center | Ujan S.A.,Shah Abdul Latif University | And 3 more authors.
Genetics and Molecular Research | Year: 2011

Qualitative trait loci (QTL) for growth and meat quality traits in cattle (Bos taurus) have been previously mapped to three chromosome regions, 0 to 30, 55 to 70, and 70 to 80 cM on chromosome 5. We evaluated the allele frequencies and gene-specific single nucleotide polymorphisms (SNPs) of bovine myogenic factor 5 (MyF-5) in the QTL regions and their associations with live weight and meat characteristics in indigenous Chinese cattle breeds. PCR-SSCP methodology showed a T>A mutation at 526 bp. Least square analysis revealed a significant association of this SNP with backfat thickness and meat tenderness (P < 0.05), while no significant association was found with live weight, loin eye height, loin eye area, rib area, or water holding capacity. Allele frequencies of MyF-5-A/B in the five breeds were 0.760/0.239, 0.752/0.247, 0.629/0.370, 0.715/0.284, and 0.750/0.250, for JiaXian red, Luxi, Nanyang, Qinchuan, and XiaNan crossbreed, respectively. The genotype distributions for these alleles in two of the Chinese cattle breeds (Luxi and Qinchuan) were not in Hardy-Weinberg equilibrium (P > 0.05); while those for the other three breeds (JiaXian red, Nanyang, and XiaNan) were in agreement with Hardy-Weinberg equilibrium (P > 0.05). The genotypic frequencies among all five cattle breeds showed moderate diversity (0.25 < polymorphism information content < 0.5). Based on our findings, we suggest that the MyF-5 gene influences back fat thickness and meat tenderness in Chinese Bos taurus. This SNP could be useful for markerassisted selection for meat quality traits in these cattle. © FUNPEC-RP www.funpecrp.com.br.

Wang H.B.,Northwest University, China | Wang H.B.,National Beef Cattle Improvement Center | Zan L.S.,Northwest University, China | Zan L.S.,National Beef Cattle Improvement Center | Zhang Y.Y.,Northwest University, China
Animal | Year: 2014

Of all the mammals of the world, the yak lives at the highest altitude area of more than 3000 m. Comparison between yak and cattle of the low-altitude areas will be informative in studying animal adaptation to higher altitudes. To investigate the molecular mechanism involved in meat quality differences between the two Chinese special varieties Qinghai yak and Qinchuan cattle, 12 chemical-physical characteristics of the longissimus dorsi muscle related to meat quality were compared at the age of 36 months, and the gene expression profiles were constructed by utilizing the bovine genome array. Significant analysis of microarrays was used to identify the differentially expressed genes. Gene ontology and pathway analysis were performed by a free Web-based Molecular Annotation System 2.0. The results reveal ~11 000 probes representing about 10 000 genes that were detected in both the Qinghai yak and Qinchuan cattle. A total of 1922 genes were shown to be differentially expressed, 633 probes were upregulated and 1259 probes were downregulated in the muscle tissue of Qinghai yak that were mainly involved in ubiquitin-mediated proteolysis, muscle growth regulation, glucose metabolism, immune response and so on. Quantitative real-time PCR (qRT-PCR) was performed to validate some differentially expressed genes identified by microarray. Further analysis implied that animals living at a high altitude may supply energy by more active protein catabolism and glycolysis compared with those living in the plain areas. Our results establish the groundwork for further studies on yaks' meat quality and will be beneficial in improving the yaks' breeding by molecular biotechnology. Copyright © The Animal Consortium 2013.

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