Time filter

Source Type

Zhao N.,Northeast Agricultural University | Xu X.-X.,Northeast Agricultural University | Wang J.,Northeast Agricultural University | Zhang Y.-J.,Shanghai Chen Guan Dairy Co | Zhang S.-Y.,National Animal Husbandry Service
Modern Food Science and Technology | Year: 2014

In this study, the inhibitory effects of bovine lactoferricin (LfcinB) and human lactoferricin (LfcinH) on the proliferation of Jurkat cells were explored and compared, by reducing the mitochondrial membrane potential in the cells. The effect on Jurkat cell proliferation was measured by MTT assay, and nuclear changes were observed by fluorescence microscopy with Hoechst 33258 staining. A flow cytometry of double-labeling by Annexin V-FITC/PI was used to distinguish the apoptosis stages in the Jurkat cells that were induced by LfcinH and LfcinB. The results showed that LfcinB and LfcinH significantly inhibited the proliferation of Jurkat cells (P < 0.05) in a dose-dependent manner. A decreased mitochondrial membrane potential in Jurkat cells was observed by JC-1 staining using laser scanning confocal microscopy. The early phase of apoptosis occurred after Jurkat cells were treated by LfcinH and LfcinB for 48 h; characteristics of apoptosis such as nuclear shrinkage and debris were observed using fluorescence microscopy. The results suggest that the change in mitochondrial membrane potential may have led to apoptosis in Jurkat cells and, eventually, the inhibition of proliferation. When the concentration of both, LfcinH and LfcinB was less than 250 μg/mL, the inhibitory effect of LfcinH on Jurkat cells was noted to be stronger; whereas at concentrations higher than 250 μg/ mL, LfcinH and LfcinB showed similar inhibitory effects. Source

Huang L.,Sichuan Agricultural University | Yan H.,Sichuan Agricultural University | Jiang X.,Sichuan Agricultural University | Yin G.,University of Arizona | And 6 more authors.
PLoS ONE | Year: 2014

Background: Quantitative real-time reverse-transcriptase PCR (qRT-PCR) is an important technique for analyzing differences in gene expression due to its sensitivity, accuracy and specificity. However, the stability of the expression of reference genes is necessary to ensure accurate qRT-PCR assessment of expression in genes of interest. Perennial ryegrass (Lolium perenne L.) is important forage and turf grass species in temperate regions, but the expression stability of its reference genes under various stresses has not been well-studied. Methodology/Principal Findings: In this study, 11 candidate reference genes were evaluated for use as controls in qRT-PCR to quantify gene expression in perennial ryegrass under drought, high salinity, heat, waterlogging, and ABA (abscisic acid) treatments. Four approaches - Delta C T, geNorm, BestKeeper and Normfinder were used to determine the stability of expression in these reference genes. The results are consistent with the idea that the best reference genes depend on the stress treatment under investigation. Eukaryotic initiation factor 4 alpha (eIF4A), Transcription elongation factor 1 (TEF1) and Tat binding protein-1 (TBP-1) were the three most stably expressed genes under drought stress and were also the three best genes for studying salt stress. eIF4A, TBP-1, and Ubiquitin-conjugating enzyme (E2) were the most suitable reference genes to study heat stress, while eIF4A, TEF1, and E2 were the three best reference genes for studying the effects of ABA. Finally, Ubiquitin (UBQ), TEF1, and eIF4A were the three best reference genes for waterlogging treatments. Conclusions/Significance: These results will be helpful in choosing the best reference genes for use in studies related to various abiotic stresses in perennial ryegrass. The stability of expression in these reference genes will enable better normalization and quantification of the transcript levels for studies of gene expression in such studies. © 2014 Huang et al. Source

YOU H.,China Agricultural University | YOU H.,China Animal Disease Control Center | ZHU J.-F.,China Agricultural University | SHE R.-P.,China Agricultural University | And 10 more authors.
Journal of Integrative Agriculture | Year: 2012

The toxicity of melamine has attracted much attention since the recent outbreaks of renal injury in pets and infants. Previous studies indicated that melamine by itself had low toxicity, whereas a mixture of melamine and cyanuric acid (M+CA) could cause serious renal damage. At present, most researches on the toxicity of M+CA are focused on the kidney. However, little is known about the adverse effects of this mixture on the reproductive system. In the present study, the toxicity of M+CA to testes was investigated. Immature male mice were orally dosed with 0, 0.6, 3, and 15 mg kg-1 d-1 of a 1:1 M+CA for 28 d. Pathological changes occurred in germ cells, such as loose arrangement, reduced numbers and karyopyknosis, indicating that this mixture was toxic to spermatogenesis. Compared with the control group, the TUNEL-positive germ cells increased significantly and the ratio of Bcl-2 to Bax, total antioxidant capacity and superoxide dismutase activity decreased significantly in the 3 and 15 mg kg-1 d-1 M+CA treated group, while the activities of caspase-3, caspase-8 and caspase-9 remained unchanged. The results suggest that M+CA can induce apoptosis in the mice testes. The downregulation of Bcl-2/Bax and oxidative stress may play a pivotal role in the induction of apoptosis by M+CA in mice testes. © 2012 Chinese Academy of Agricultural Sciences. Source

Qiang H.,Chinese Academy of Sciences | Chen Z.,National Animal Husbandry Service | Zhang Z.,Chinese Academy of Sciences | Wang X.,Chinese Academy of Sciences | And 2 more authors.
PLoS ONE | Year: 2015

Information on genetic diversity and population structure of a tetraploid alfalfa collection might be valuable in effective use of the genetic resources. A set of 336 worldwide genotypes of tetraploid alfalfa (Medicago sativa subsp. sativa L.) was genotyped using 85 genome-wide distributed SSR markers to reveal the genetic diversity and population structure in the alfalfa. Genetic diversity analysis identified a total of 1056 alleles across 85 marker loci. The average expected heterozygosity and polymorphism information content values were 0.677 and 0.638, respectively, showing high levels of genetic diversity in the cultivated tetraploid alfalfa germplasm. Comparison of genetic characteristics across chromosomes indicated regions of chromosomes 2 and 3 had the highest genetic diversity. A higher genetic diversity was detected in alfalfa landraces than that of wild materials and cultivars. Two populations were identified by the model-based population structure, principal coordinate and neighbor-joining analyses, corresponding to China and other parts of the world. However, lack of strictly correlation between clustering and geographic origins suggested extensive germplasm exchanges of alfalfa germplasm across diverse geographic regions. The quantitative analysis of the genetic diversity and population structure in this study could be useful for genetic and genomic analysis and utilization of the genetic variation in alfalfa breeding. © 2015 Qiang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Source

Li M.,Sichuan Agricultural University | Li M.,Tsinghua University | Tian S.,Novogene Bioinformatics Institute | Jin L.,Sichuan Agricultural University | And 49 more authors.
Nature Genetics | Year: 2013

We report the sequencing at 131× coverage, de novo assembly and analyses of the genome of a female Tibetan wild boar. We also resequenced the whole genomes of 30 Tibetan wild boars from six major distributed locations and 18 geographically related pigs in China. We characterized genetic diversity, population structure and patterns of evolution. We searched for genomic regions under selection, which includes genes that are involved in hypoxia, olfaction, energy metabolism and drug response. Comparing the genome of Tibetan wild boar with those of neighboring Chinese domestic pigs further showed the impact of thousands of years of artificial selection and different signatures of selection in wild boar and domestic pig. We also report genetic adaptations in Tibetan wild boar that are associated with high altitudes and characterize the genetic basis of increased salivation in domestic pig. Source

Discover hidden collaborations