PubMed | Instituto Zooprofilattico Dellabruzzo E Del Molise G Caporale National And Oie Reference Laboratory For Brucellosis
Type: | Journal: Methods in molecular biology (Clifton, N.J.) | Year: 2014
The multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA) is a genetic typing method based on the evaluation of the number of repeated sequences in multiple selected loci of microbial DNA. Although several MLVA typing panels have been proposed for brucellae, the 16-loci panel is recognized as the standard genotyping method, also used for the Brucella international online database. This chapter describes a high-throughput MLVA-16 protocol using multiplex PCRs and multicolor capillary electrophoresis.