National Agriculture Research Institute of tunisia INRAt

El Kef, Tunisia

National Agriculture Research Institute of tunisia INRAt

El Kef, Tunisia
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Bchini H.,National Agriculture Research Institute of Tunisia INRAT | Naceur M.B.,National Agriculture Research Institute of Tunisia INRAT | Sayar R.,Kef Research Station Boulifa | Khemira H.,Gabes University
Hereditas | Year: 2010

In water-limited areas of Tunisia, more than 30% of subsurface water used for irrigation is saline water, leading to a long-term salinization and degradation processes. To prevent the problems and to minimize the negative impact of using saline water, selection of proper germplasm as well as integrated soil and water management are essential. Understanding the diversity for salt tolerance in barley (Hordeum vulgare L.) genotypes will facilitate their use in genetic improvement. Our objectives were to evaluate, in green, house-salt tolerance for 14 barley cultivars under three different salinity levels: (tap water with an Ec = 0.73 dSm-1, tap water with 102 mM of NaCl, Ec = 10.76 dSm-1 and tap water with 151 mM of NaCl, Ec = 15.38 dSm-1), and quantify genetic variation based on salt tolerance index, morphological traits, molecular and factorial analysis of correspondence (FAC). The study was conducted in a randomized complete block design arranged as a split plot. The results indicate a great genetic variability to salt tolerance among used barley genotypes. Consequently, it is possible to identify superior cultivars, and evaluate the genotypic performance under salinities conditions. In addition, results obtained were confirmed by clustering made by SSR tool on the base of DNA analysis, which is compatible with the arrangement obtained with statistical method. © 2010 The Authors.


Sayar R.,National Agriculture Research Institute of Tunisia INRAT | Bchini H.,Physiology Laboratory | Mosbahi M.,Banque Nationale de Genes | Ezzine M.,Physiology Laboratory
African Journal of Agricultural Research | Year: 2010

The present investigation has been performed to evaluate Durum wheat (Triticum durum Desf.) tolerance to osmotic stress at the water potentials of -0.2, -0.4, -0.6 and -0.8 MPa induced by polyethylene glycol PEG-8000 (polyethylene glycol 8000) or NaCI solutions at an early stage of plant growth. Each, on germination, emergence and early seedling growth, were investigated for two Durum wheat (Triticum durum Desf.) varieties differing in drought tolerance (BD290273 and Omrabia). Daily and final germination and emergence percentage, as well as germination and seedling emergence rate, and seedling fresh and dry weight were measured in the study under controlled conditions. Results showed those germination and emergence rates were delayed by both solutions in both varieties, with differences between genotypes among growth stages, given that Omrabia variety showed a higher germination and emergence rate than BD290273 genotype in NaCI but BD290273 was less affected by NaCI and PEG solutions at the emergence stage. Sodium chloride had a lesser effect on varieties in terms of germination and emergence rates, the final germination and the emergence percentage than did PEG-8000. This conclusively proves that the adverse effect of PEG on germination, emergence and early seedling growth was due to the osmotic effect rather than the specific ion. Seedling growth was reduced by both stresses. But NaCI usually caused less damage than PEG to Durum wheat seedlings, suggesting that NaCI and PEG acted through different mechanisms. This difference in cultivar's behaviour according to the growth conditions is discussed. It was concluded that inhibition in germination at equivalent water potentials of NaCI and PEG-8000 was mainly due to an osmotic effect rather than did salt toxicity. ©2010 Academic Journals.


Sayar R.,National Agriculture Research Institute of tunisia INRAt | Bchini H.,National Agriculture Research Institute of tunisia INRAt | Mosbahi M.,National Agriculture Research Institute of tunisia INRAt | Khemira H.,Gabes University
Czech Journal of Genetics and Plant Breeding | Year: 2010

Two durum wheat (triticum durum Desf.) cultivars were tested for salt and drought tolerance at germination, seedling emergence and early seedling growth in NaCl and PEG-8000 solutions of different osmotic potentials (-0.2, -0.4, -0.6 and -0.8 MPa). Daily and final germination and emergence percentage, as well as germination and seedling emergence rate, seedling growth, fresh and dry weight were recorded under controlled conditions. Results showed that germination and emergence rates were delayed by both solutions in both cultivars, but Omrabia showed higher germination and emergence rates than BD290273 in NaCl while BD290273 was less affected by NaCl and PEG solutions at the emergence stage. Sodium chloride had a lesser effect on both cultivars in terms of germination rate, emergence rate, final germination and emergence percentage than did PEG-8000. This conclusively proves that the adverse effect of PEG-8000 on germination, emergence and early seedling growth was due to the osmotic effect rather than to the specific ion. Seedling growth was reduced by both stresses. However, NaCl usually caused less damage than PEG to durum wheat seedlings, suggesting that NaCl and PEG acted through different mechanisms.


PubMed | National Agriculture Research Institute of Tunisia INRAT
Type: Journal Article | Journal: Hereditas | Year: 2010

In water-limited areas of Tunisia, more than 30% of subsurface water used for irrigation is saline water, leading to a long-term salinization and degradation processes. To prevent the problems and to minimize the negative impact of using saline water, selection of proper germplasm as well as integrated soil and water management are essential. Understanding the diversity for salt tolerance in barley (Hordeum vulgare L.) genotypes will facilitate their use in genetic improvement. Our objectives were to evaluate, in green, house-salt tolerance for 14 barley cultivars under three different salinity levels: (tap water with an Ec = 0.73 dSm(-1), tap water with 102 mM of NaCl, Ec = 10.76 dSm(-1) and tap water with 151 mM of NaCl, Ec = 15.38 dSm(-1)), and quantify genetic variation based on salt tolerance index, morphological traits, molecular and factorial analysis of correspondence (FAC). The study was conducted in a randomized complete block design arranged as a split plot. The results indicate a great genetic variability to salt tolerance among used barley genotypes. Consequently, it is possible to identify superior cultivars, and evaluate the genotypic performance under salinities conditions. In addition, results obtained were confirmed by clustering made by SSR tool on the base of DNA analysis, which is compatible with the arrangement obtained with statistical method.

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