Lian S.,Seoul National University |
Lee J.-S.,Seoul National University |
Cho W.K.,Seoul National University |
Yu J.,Seoul National University |
And 3 more authors.
PLoS ONE | Year: 2013
Tomato spotted wilt virus (TSWV) severely damages and reduces the yield of many economically important plants worldwide. In this study, we determined the whole-genome sequences of 10 TSWV isolates recently identified from various regions and hosts in Korea. Phylogenetic analysis of these 10 isolates as well as the three previously sequenced isolates indicated that the 13 Korean TSWV isolates could be divided into two groups reflecting either two different origins or divergences of Korean TSWV isolates. In addition, the complete nucleotide sequences for the 13 Korean TSWV isolates along with previously sequenced TSWV RNA segments from Korea and other countries were subjected to phylogenetic and recombination analysis. The phylogenetic analysis indicated that both the RNA L and RNA M segments of most Korean isolates might have originated in Western Europe and North America but that the RNA S segments for all Korean isolates might have originated in China and Japan. Recombination analysis identified a total of 12 recombination events among all isolates and segments and five recombination events among the 13 Korea isolates; among the five recombinants from Korea, three contained the whole RNA L segment, suggesting reassortment rather than recombination. Our analyses provide evidence that both recombination and reassortment have contributed to the molecular diversity of TSWV. © 2013 Lian et al.
Akter S.,Kyung Hee University |
Jo H.,Kyung Hee University |
Du J.,Kyung Hee University |
Won K.H.,Kyung Hee University |
And 6 more authors.
Archives of Microbiology | Year: 2015
A novel bacterial strain THG-MM13T was isolated from rhizospheric soil sample and was characterized by using a polyphasic approach. Cells were Gram-reaction-negative, non-motile and rod-shaped. The strain was aerobic, catalase and oxidase positive, and optimum growth temperature and pH were 28 °C and 7.0, respectively. On the basis of 16S rRNA gene sequence analysis, strain THG-MM13T (KM598260) belongs to the genus Pseudoxanthomonas and is most closely related to Pseudoxanthomonas wuyuanensis KCTC 23877T (97.4 %) (JN247803), followed by Pseudoxanthomonas koreensis KCTC 12208T (96.7 %) (AY550263) and Pseudoxanthomonas yeongjuensis KACC 11580T (96.7 %) (DQ438977). The DNA G + C content was 63.7 mol%, and the predominant respiratory quinone was ubiquinone-8. The major polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. The major fatty acids were iso-C15:0 (31.3 %) and iso-C16:0 (19.3 %). The DNA–DNA relatedness value between strain THG-MM13T and P. wuyuanensis KCTC 23877T was below 50 %. The DNA–DNA hybridization result and results of the genotypic analysis in combination with chemotaxonomic and physiological data demonstrated that strain THG-MM13T represented a novel species within the genus Pseudoxanthomonas, for which the name Pseudoxanthomonas humi is proposed. The type strain is THG-MM13T (=KACC 18280T = CCTCC AB 2015122T). © 2015, Springer-Verlag Berlin Heidelberg.
Park J.-H.,Chonbuk National University |
Lee S.-M.,Jeonju Hanil High School |
Lee S.-G.,National Academy of Agriculture Science |
Lee H.-S.,Chonbuk National University
Journal of Applied Biological Chemistry | Year: 2014
This study was conducted to determine the attractive effects of Plutella xylostella adults to light emitting diode (LED) trap in greenhouse and compared with those of no light trap and black-light which is typically used in commercial luring lamp. The green LED trap captured more P. xylostella when compared with black-light trap, whereas the no light trap was a little attractive to P. xylostella adults. These results indicated that the green LED traps could be used for environmental insect pest control. © The Korean Society for Applied Biological Chemistry 2014.
Jeon J.-H.,Chonbuk National University |
Lee S.-G.,Chonbuk National University |
Lee H.-S.,National Academy of Agriculture Science
Journal of Applied Biological Chemistry | Year: 2014
To evaluate light-emitting diode (LED) as potential attractants for Bemisia tabaci adults, attractiveness of white and yellow LED traps were investigated in greenhouse. The yellow LED trap showed the most attractive to B. tabaci adults, followed by a similarly attraction to the white LED trap, whereas the control (no light trap) was little attractive to B. tabaci adults. These results suggested that yellow and white LED traps could be used for environment-friendly insect pest control. © The Korean Society for Applied Biological Chemistry 2014.
Lee S.-E.,Kyungpook National University |
Young-Woong C.,Korea University |
Mo H.-H.,Korea University |
Son J.,Korea University |
And 2 more authors.
International Journal of Biological Sciences | Year: 2013
The objective of this study was to find and validate estrogen-related biomarkers from plasma proteins in Oryzias latipes after exposure to an estrogen disrupting compound, α-endosulfan. The acute toxicity of α-endosulfan on O. latipes after 96 h of exposure was 13.72, 16.18, and 22.18 μg L-1 for the LC10, LC20, and LC50 values, respectively. To confirm estrogenic disturbance by α-endosulfan, the expression level of vitellogenin in the liver of male fishes was measured at the LC10 value, and it was found to be significantly different from the reference group, confirming the estrogenic effect of endosulfan in this concentration range. Proteinchip® array techniques using a weak cation exchange (CM10) and a strong anion exchange proteinchip (Q10) in conjunction with surface-enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) were used to determine plasma proteins of O. latipes differently expressed in response to endosulfan exposure at LC10 and LC20 concentrations. Analysis of protein profiling of the male fish exposed to α-endosulfan detected 48 significantly different protein peaks and the proteins at m/z 2819, 8462, 8860, and 9462 were significantly different (p<0.05). The protein peaks at m/z 2819, 8860, and 9462 were up-regulated and the peak at m/z 8462 was down-regulated. Therefore, these four differentially expressed proteins could be used as biomarkers to rapidly determine a possible risk of endosulfan on aquatic ecosystems, although these are not necessarily produced as a result of endocrine disruption. © Ivyspring International Publisher.
PubMed | National Academy of Agriculture Science and Chonbuk National University
Type: Journal Article | Journal: Journal of food protection | Year: 2015
Isolates from essential oil extracted from the flowers and leaves of Ruta graveolens and commercial phenolic analogs were evaluated using fumigant and contact toxicity bioassays against adults of the stored-food pests Sitophilus zeamais, Sitophilus oryzae, and Lasioderma serricorne. The insecticidal activity of these compounds was then compared with that of the synthetic insecticide dichlorvos. To investigate the structure-activity relationships, the activity of 2-isopropyl-5-methylphenol and its analogs was examined against these stored-food pests. Based on the 50% lethal dose, the most toxic compound against S. zeamais was 3-isopropylephenol, followed by 2-isopropylphenol, 4-isopropylphenol, 5-isopropyl-2-methylphenol, 2-isopropyl-5-methylphenol, 3-methylphenol, and 2-methylphenol. Similar results were observed with phenolic compounds against S. oryzae. However, when 2-isopropyl-5-methylphenol isolated from R. graveolens oil and its structurally related analogs were used against L. serricorne, little or no insecticidal activity was found regardless of bioassay. These results indicate that introducing and changing the positions of functional groups in the phenol skeleton have an important effect on insecticidal activity of these compounds against stored-food pests.