Li N.,Natioiud Institute for Radiologic Protection |
Wang C.-Y.,Natioiud Institute for Radiologic Protection |
Zheng H.,Natioiud Institute for Radiologic Protection |
Shao S.,Natioiud Institute for Radiologic Protection |
And 4 more authors.
Chinese Journal of Biologicals | Year: 2015
Objective To investigate the inhibitory effect of cerium oxide nanoparticles (nm-CeO2) on the growth of human breast carcinoma Bcap-37 cell xenografts in nude mice. Methods The tumor-bearing nude mouse model was established by transplantion of human breast carcinoma Bcap-37 cells into right infra axillary dermis. BALB/e-nu mice bearing Bcap-37 cells were randomly divided into negative control, positive control and three test groups. The mice in three test groups were treated with nm-CeO2 at high (300 mg/kg), middle (100 mg/kg) and low (33.33 mg/kg) dosages by lavage respectively, while those in negative control group with double distilled water at an equal volume, and those in positive group with Cordyceps kyushuensis at a dosage of 2 000 mg/kg, once a day for 17 d. The growths of transplanted tumors in various groups were observed, while the tumor sizes (VT) were measured, the inhibiting rates of tumor growth were calculated, the tumors were weighed, the pathological changes of transplanted tumors were observed, and the apoptosis rates of transplanted tumor cells were determined by terminal dexynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) method. Results Compared with those in negative control group, the tumor growths in three test groups were inhibited significantly, while the degeneration, necrosis and apoptosis of tumor cells were promoted (P < 0.05). The inhibiting rate of tumor growth in mice treated with nm-CeO2 at high dosage was significantly lower than that in positive control group (P < 0.05). However, the apoptosis rates of tumor cells treated with nm-CeO2 at high and middle dosages were significantly higher than that in positive control group (P < 0.05). Conclusion The nm-Ce02 inhibited the growth of human breast carcinoma Bcap-37 cell xenografts in nude mice significantly.