NARIC Research Institute for Animal Breeding Nutrition and Meat Science

Hungary

NARIC Research Institute for Animal Breeding Nutrition and Meat Science

Hungary
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Zsolnai A.,NARIC Research Institute for Animal Breeding Nutrition and Meat Science | Zsolnai A.,University of Kaposvár | Szanto-Egesz R.,Biomi Ltd. | Ferencz-Elblinger E.,University of Kaposvár | And 4 more authors.
Acta Alimentaria | Year: 2017

We used an alternative approach, loop-mediated isothermal amplification, to detect Mangalitza component in food products, and it has been compared to an established Recombinase Polymerase Amplification test. The correlation between the assays was significant P<0.01). Linear determination coefficient between the assays was 0.993 and level of diagnostic agreement was high (Kappa=0.971). Previously, a real-time PCR method based on TaqMan probe was developed (SZÁNTÓ-EGÉSZ et al., 2013) for detection of Mangalitza meat in food products, using a Mangalitza specific sequence. Other Mangalitza specific sequences suitable for the same purpose are also in use (V. STÉGER, personal communication). Approaches like real-time monitoring of accumulation of the specific DNA product usually require specialised laboratory equipment. For Mangalitza detection, portable Recombinase Polymerase Amplification (RPA) approach has been developed (SZÁNTÓ-EGÉSZ et al., 2016), which requires a device capable of maintaining 39 °C and a lateral flow strip with easy yes/no indication of the successful amplification. We wanted to develop another fast, non-PCR based test with minimal laboratory requirement to provide a third possibility to detect Mangalitza component in food. © 2017 The Author(s).


Szanto-Egesz R.,Biomi Ltd. | Janosi A.,NARIC Food Science Research Institute | Mohr A.,Biomi Ltd. | Szalai G.,University of South Carolina | And 6 more authors.
Food Analytical Methods | Year: 2016

A fast and reliable diagnostic system has been developed for the detection of Mangalica meat in foods. This qualitative test is based on a recombinase polymerase amplification which can be performed on the field, in situ, where it may be necessary to determine Mangalica content in food products at once. The required equipments for the procedure are pipettes, a portable homogenizer and a portable thermostat. DNA amplification is carried out at a constant temperature, and the detection is based on antibody reaction. The detection limit is one copy of the target sequence in 1 μl reaction volume. The test can be used for uncovering falsification of local brands on the spot within a very short (25–45 min) period of time. The present approach can be adopted for the detection of other food ingredients, if the species-specific target DNA sequence is known, e.g. in case of chicken, turkey, horse, and cattle. © 2015, Springer Science+Business Media New York.

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