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Hiraizumi S.,Livestock Research Institute | Nishinomiya H.,Akita | Oikawa T.,Miyagi Prefectural Livestock Experiment Station | Sakagami N.,Kanagawa Prefectural Livestock Technology Center | And 7 more authors.
Theriogenology | Year: 2015

To reduce labor for superovulation treatment by twice-daily intramuscular (im) administration of FSH for more than 3 to 4days, we investigated the superovulatory responses of Japanese Black cows to porcine FSH (pFSH) used as a single subcutaneous (sc) administration at two different doses in two different volumes of saline. In experiment 1, 20 Armour units (AU) of pFSH dissolved in either 10mL (treatment A; n=14) or 50mL (treatment B; n=14) of saline was administered subcutaneously in the neck region. In experiment 2, 30AU of pFSH dissolved in either 10mL (treatment C; n=15) or 50mL (treatment D; n=15) of saline was administered subcutaneously in the neck region. The control animals in experiment 1 (n=14) and experiment 2 (n=15) received 20AU of pFSH administered intramuscularly twice daily in decreasing doses for more than 3days. In experiment 1, mean (±SEM) numbers of CL (15.4±2.5, 18.1±3.4, and 17.2±2.6), total number of ova and embryos (12.9±1.4, 15.9±3.5, and 16.2±2.8), and transferable embryos (7.5±2.0, 10.4±2.8, and 8.0±2.1) did not differ among treatments A, B, and control. In experiment 2, mean (±SEM) numbers of CL (20.5±4.3, 20.4±2.7, and 20.1±3.4), total number of ova and embryos (21.7±4.2, 17.3±3.4, and 16.5±3.2), and transferable embryos (8.1±1.6, 9.3±2.2, and 9.5±1.9) did not differ among treatments C, D, and control. Although there were no differences in serum pFSH concentrations among the three treatments at each of the time points in experiment 1, in experiment 2, the serum pFSH concentration at 6 and 8hours after pFSH administration in treatment C (3.1±0.8, 2.7±0.5ng/mL, mean±SEM) was significantly greater (P<0.05) than in the control (0.7±0.1, 1.1±0.2ng/mL). At 10hours after administration, the pFSH concentration had decreased and there were no differences among the three treatments at subsequent time points. These results suggest that increasing the volume of saline or the dose of pFSH does not affect the absorption pattern of pFSH administered as a single sc administration. In conclusions, single sc administration of pFSH at a dose of 20 or 30 AU dissolved in 10 or 50mL of saline is able to induce a superovulatory response comparable with that obtained by twice-daily im administration in Japanese Black cows. © 2015 Elsevier Inc. Source


Yasumatsuya K.,Research Institute of Environment Agriculture and Fisheries | Kasai K.,Research Institute of Environment Agriculture and Fisheries | Yamanaka K.,Shiga Prefectural Livestock Technology Promotion Center | Nishino O.,Nara Prefectural Livestock Technology Center | And 4 more authors.
Livestock Science | Year: 2012

Data from 63 Japanese Black calves were collected to clarify the effects of feeding whey protein on the growth rate and mucosal IgA induction in calves. Dietary treatments in milk replacers were 1) 26% CP as in skim milk (control), 2) 26% CP as whey and skim milk and 3) 26% CP as whey. Diets were offered from 3 to 63. days of age in calves. Feeding whey protein had no effects on growth rate, fecal consistency and fecal water in calves. Compared with 2. days of age, fecal IgA concentration in calves decreased at 14. days of age, while fecal water increased. Feeding whey protein increased fecal IgA in calves after 14. days of age, which was thought to be the increased mucosal IgA induction in the gut. Serum cholesterol concentration tended to be lower in calves fed whey than in control group, but feeding whey protein had no clear effects on serum glucose, NEFA, total protein and urea-N concentrations. These results suggest that feeding whey protein enhances mucosal IgA induction in calves, but feeding whey protein has little effect on growth rate and fecal consistency in calves. © 2011 Elsevier B.V. Source


Sakagami N.,Kanagawa Agricultural Technology Center | Umeki H.,Forestry and Fisheries Research Center Livestock Research Institute | Nishino O.,Nara Prefectural Livestock Technology Center | Uchiyama H.,Miyazaki Prefectural Livestock Research Institute | And 6 more authors.
Journal of Reproduction and Development | Year: 2012

The objective of this study was to examine whether high concentrations of epidermal growth factor (EGF) and/or insulin-like growth factor I (IGF-I) would have a beneficial effect on bovine embryo development in vitro and to obtain normal calves by using an ovum pick up method and embryo culture in a chemically defined medium. When compared with controls, EGF (100 or 200 ng/ml) or IGF-I (50 or 100 ng/ml) significantly increased the rate of embryos that developed into blastocysts during an 8-day culture after the in vitro fertilization of oocytes obtained from ovaries from a slaughterhouse. IGF-I induced a dose-dependent increase in cell number in both the inner cell mass and the trophectoderm, whereas EGF stimulated proliferation only in the inner cell mass. A combination of EGF (100 ng/ml) and IGF-I (50 ng/ml) produced an additive effect, and embryos developed into blastocysts at a comparatively high rate (27.9%) compared with controls (12.0%). A similar rate of development was achieved using a combination of EGF and IGF-I in the culture of embryos following ovum pick up by ultrasound-guided transvaginal follicular aspiration and in vitro fertilization, and 5 blastocysts that developed after the culture were transferred into uteri; two embryos implanted, and normal calves were born. These results suggest that the combined use of EGF and IGF-I makes bovine embryo culture in a chemically defined medium a practical and useful procedure for producing blastocysts, and its application to embryo culture following ovum pick up and in vitro fertilization could be useful for producing normal calves. © 2012 by the Society for Reproduction and Development. Source


Nishiyama Y.,Kyoto University | Yasumatsuya K.,Research Institute of Environment | Kasai K.,Research Institute of Environment | Nishino O.,Nara Prefectural Livestock Technology Center | And 5 more authors.
Livestock Science | Year: 2011

Data from 17 pregnant mice and 33 Japanese Black calves were collected to clarify the effect of supplemental β-carotene with whey on IgA transfer from maternal milk and mucosal IgA induction in neonatal mice and calves. Dietary treatments in milk replacers were 1) 26% CP as in skim milk (control), 2) 26% CP as whey and 3) 26% CP as whey and 30. mg/kg β-carotene. Diets were offered from 6.5. days postcoitus to 14. days postpartum in pregnant mice and from 3 to 63. days postpartum in calves. Supplemental β-carotene with whey increased the numbers of IgA antibody secreting cells (ASC) in the mammary gland in maternal mice and IgA concentration in stomach contents in neonatal mice at 14. days postpartum, which was the consequence of the higher IgA transfer from maternal milk to neonates. The number of IgA ASC in the mammary gland in maternal mice fed whey was higher than that of control mice, but intestinal IgA concentration of neonatal mice was not affected by treatments. Supplemental β-carotene with whey drastically increased serum β-carotene concentration in calves at 14 and 42. days postpartum. Supplemental β-carotene with whey had no effects on fecal IgA concentration and fecal water in calves. These results suggest that β-carotene supplementation with whey to maternal mice during pregnancy and lactation enhances IgA transfer from maternal milk to neonates, but supplemental β-carotene has little effect on mucosal IgA induction in neonatal mice and calves. © 2010 Elsevier B.V. Source

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