Nanxishan Hospital of Guangxi Zhuang Autonomous Region

Guilin, China

Nanxishan Hospital of Guangxi Zhuang Autonomous Region

Guilin, China
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Jiang W.,Guangxi Medical University | Huang W.,Guangxi Medical University | Chen Y.,Nanxishan Hospital of Guangxi Zhuang Autonomous Region | Zou M.,Guangxi Medical University | And 2 more authors.
Oxidative Medicine and Cellular Longevity | Year: 2017

Amyloid beta (Aβ) deposition is increased in human immunodeficiency virus-1-(HIV-1-) infected brain, but the mechanisms are not fully understood. The aim of the present study was to evaluate the role of Ras signaling in HIV-1 transactivator protein-(Tat-) induced Aβ accumulation in human cerebral microvascular endothelial cells (HBEC-5i). Cell viability assay showed that 1 μg/mL Tat and 20 μmol/L of the Ras inhibitor farnesylthiosalicylic acid (FTS) had no significant effect on HBEC-5i cell viability after 24 h exposure. Exposure to Tat decreased protein and mRNA levels of zonula occludens-(ZO-) 1 and Aβ-degrading enzyme neprilysin (NEP) in HBEC-5i cells as determined by western blotting and quantitative real-time polymerase chain reaction. Exposure to Tat also increased transendothelial transfer of Aβ and intracellular reactive oxygen species (ROS) levels; however, these effects were attenuated by FTS. Collectively, these results suggest that the Ras signaling pathway is involved in HIV-1 Tat-induced changes in ZO-1 and NEP, as well as Aβ deposition in HBEC-5i cells. FTS partially protects blood-brain barrier (BBB) integrity and inhibits Aβ accumulation. © 2017 Wenlin Jiang et al.


Wang Y.,Guilin Medical University | Weng H.,Huazhong University of Science and Technology | Song J.F.,Guilin Medical University | Deng Y.H.,Huazhong University of Science and Technology | And 2 more authors.
Molecular Medicine Reports | Year: 2017

High mobility group protein B1 (HMGB1) has been reported to serve important roles in various pathological conditions. Toll-like receptor 4 (TLR4), as one of the HMGB1 receptors, has been reported to be involved in the development of certain inflammatory diseases by activating nuclear factor NF-κ-B (NF-κB). However, there are few studies investigating the effects of HMGB1, TLR4 and NF-κB on human inflammatory dermatoses. In the present study, the distribution and characteristics of HMGB1, TLR4 and NF-κB p65 expression in psoriasis and atopic eczema (AE) were investigated. In addition, immunohistochemical analysis was performed to evaluate their expression and distribution in normal skin, and in patients with AE or psoriasis. Spearman's correlation analysis was used to predicate their relevancy. The present study identified that the p65 level in epithelial nuclei in AE skin was increased compared with normal and psoriasis skin (P<0.01). The level of extracellular HMGB1 in AE skin was also increased compared with normal and psoriasis skin (P<0.01). Meanwhile, TLR4 expression on the epithelial membranes of AE skin was increased compared with psoriasis skin (P<0.01). Furthermore, the level of extracellular HMGB1 was positively correlated with epithelial membrane TLR4 (r=0.3856; P<0.05) and epithelial nuclear p65 (r=0.5894; P<0.01) in AE skin. These results indicated that the HMGB1-TLR4-NF-κB signaling pathway is activated in AE and may account for its pathogenesis, but not in psoriasis. Therefore, HMGB1, TLR4 and NF-κB p65 have the potential to be targets for the treatment of human inflammatory dermatoses, including AE.


Liu H.-H.,Guangxi Medical University | Liu H.-H.,Nanxishan Hospital of Guangxi Zhuang Autonomous Region | Wang J.,Nanxishan Hospital of Guangxi Zhuang Autonomous Region | Chen X.-M.,Guangxi Medical University | And 3 more authors.
European Review for Medical and Pharmacological Sciences | Year: 2016

OBJECTIVE: Many neuroimaging studies have shown that temporal lobe epilepsy (TLE) is associated with functional and structural abnormalities at specific brain areas. Unfortunately, relatively limited information has been presented about the alterations of interhemi-spheric functional and anatomic connectivity in patients with unilateral TLE. In the present study, we investigated interhemispheric functional connectivity using a voxel-mirrored homo-topic connectivity (VMHC) method. We further revealed fractional anisotropy (FA) changes in the areas with abnormal VMHC values in TLE patients by diffusion tensor imaging (DTI). Moreover, their relationships with alertness in patients with drug-naïve unilateral TLE were also investigated. PATIENTS AND METHODS: Forty-three patients with unilateral TLE (21 left TLE and 22 right TLE) and 20 normal controls (NC) were recruited for case-control study. All of the subjects underwent acquisition of resting-state functional magnetic resonance images, Mini-Mental State Examination (MMSE) and the attention network test. DTI images were collected in 26 patients with unilateral TLE (10 left TLE and 16 right TLE) and 20 NCs. Functional connectivity between bilateral homotopic voxels was calculated. Homotopic regions showing abnormal functional connectivity in patients were adopted as regions of interest for the analysis of DTI. The FA values, MMSE scores, and alertness were compared between groups. Correlation analyses were employed to examine the relationships between each radiographic parameter (VMHC and FA) and each clinical and neuropsychological parameter in patients with drug-naïve unilateral TLE. RESULTS: Compared with NC, patients with left TLE exhibited significantly higher VMHC values in the bilateral angular gyrus, inferior occipital gyrus and superior parietal gyrus and lower VMHC values in the bilateral supplementary motor area, inferior parietal lobule, middle temporal gyrus, and medial superior frontal gyrus. In patients with right TLE, higher VMHC values were found in the bilateral inferior occipital gyrus, parahippocampal gyrus and cerebellum; lower VMHC values were observed in the bilateral middle temporal gyrus and precentral gyrus/inferior frontal gyrus. FA values of the commissural fiber bundles connecting the bilateral parahip-pocampal gyrus were smaller in the right TLE than those in the NC group. Meanwhile, the alerting effect of patients was determined to be impaired and positively correlated with FA values of the commissural fiber bundles connecting the bilateral parahippocampal gyrus in right TLE patients. CONCLUSIONS: Our findings indicate that the bilateral parahippocampal gyrus may be important to the pathophysiology of patients with drug-naïve unilateral TLE. The significant correlation between the FA values and alertness indicates that structural changes are involved in the alterations in the alertness network in unilateral right TLE patients.


Hong Y.,Anhui Medical University | Hong Y.,Nanxishan Hospital of Guangxi Zhuang Autonomous Region | Qiao J.-L.,Nanxishan Hospital of Guangxi Zhuang Autonomous Region | Qiao J.-L.,Xingtai Peoples Hospital Of Hebei Province | And 6 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2016

Purpose: Understanding the difference of expression of FAS between breast cancer and benign lesion tissues of breast and discuss the expression of FAS in tissues of breast cancer and its clinical value. Methods: FAS mRNA and protein expression were determined by quantitative real-time reverse transcriptase-polymerase chain reaction and Western blotting in 20 pairs of fresh frozen breast cancer tissues, corresponding noncancerous tissues and normal tissues. Additionally, FAS expression was analyzed by immunohistochemistry in 108 cases of clinicopathologically characterized breast cancer patients. The correlation of FAS expression with patients’ survival rate was assessed by Kaplan-Meier. Results: The result shows that expression of FAS in mRNA and protein in the breast benign lesion is low level while in the noncancerous tissues is high, but in the tissues of breast cancer is revealed as excessive expression. FAS expression positive rate and staining intensity within the cancer tissues is higher than that of breast lesions (P<0.01). The high expression of FAS is significantly correlated with the diameter of tumor of the breast cancer (P=0.014) and transferring quantity of the surrounding lymph node (P=0.047). There is no statistical correlation found between FAS and ages of patients, menopause or not, tumor tissue ER, PR, c-erbB-2 (P>0.05). Kaplan-Meier survival analysis showed that a high expression level of FAS resulted in a significantly poor prognosis of breast cancer patients. Conclusion: In conclusion, overexpression of FAS is closely related to progression of breast cancer and might be regarded as an independent predictor of poor prognosis for breast cancer. © 2016, E-Century Publishing Corporation. All rights reserved.


Liu H.-H.,Guangxi Medical University | Liu H.-H.,Nanxishan Hospital of Guangxi Zhuang Autonomous Region | Wang J.,Nanxishan Hospital of Guangxi Zhuang Autonomous Region | Chen X.-M.,Guangxi Medical University | And 3 more authors.
Medicine (United States) | Year: 2016

In the present study, we adopted a novel method - local diffusion homogeneity (LDH) - to characterize the structure feature in mesial temporal lobe epilepsy (MTLE). Diffusion-weighted images were acquired from 11 left MTLE patients, 16 right MTLE patients, and 20 healthy controls from May 2014 to January 2015. Local diffusion homogeneity was compared among patient groups and controls by 2 sample t test. The discriminative value of LDH abnormalities was examined by receiver operating characteristic (ROC) curve analysis. Correlations with disease duration and onset age in both patient groups were assessed using Pearson's coefficient. Both patient groups exhibited lower LDH in the anterior corpus callosum (P < 0.05, corrected), and this regional anomaly exhibited excellent classification performance in left MTLE patients (sensitivity = 82%, specificity = 100%), right MTLE patients (sensitivity = 81%, specificity = 90%), and the entire patient cohort (sensitivity = 82%, specificity = 95%). In summary, left and right MTLE patients show common pathological changes in the anterior corpus callosum. This regional LDH abnormality is a potential quantitative biomarker for MTLE. Copyright © 2016 the Author(s). Published by Wolters Kluwer Health, Inc. All rights reserved.


Zhang J.,Guilin Medical University | Zhang G.,Soochow University of China | Yang S.,Guilin Medical University | Qiao J.,Guilin Medical University | And 2 more authors.
World Journal of Surgical Oncology | Year: 2016

Background: As a kind of versatility of cytokines, overexpression of macrophage migration inhibitory factor (MIF) and vascular endothelial growth factor-C (VEGF-C) have been reported in a wide variety of tumors. However, the correlation and mechanism between MIF and VEGF-C are still not clear. As an important signal transduction system, MAPK signaling pathways participate in a variety of biological behavior of cells. The purposes of this study are to study the relationship between MIF and VEGF-C and discuss the role of MAPK signal pathway in the relationship. Methods: In this study, we first knocked down the MIF using small interfering RNA (siRNA) and built the stable low expression MIF breast cancer cells (siRNA-MIF-MCF-7) and the negative control cells (siRNA-NC-MCF-7). And then, we evaluated the expression of MIF using Western blot to confirm the effect of transfection. Using real-time fluorescent quantitative polymerase chain reaction and enzyme-linked immunosorbent experiment, we respectively examined the different expression of VEGF-C between siRNA-MIF-MCF-7 and siRNA-NC-MCF-7 and breast cancer cells MCF-7. Moreover, we investigated the expression of p38 MAPK, P-p38 MAPK, p44/42 MAPK, and P-p44/42 MAPK in the three kinds of cells by Western blot to analyze the regulatory mechanism to VEGF-C. Results: We found that MIF siRNA markedly reduced the expression of MIF. And the expression level of VEGF-C, p38 MAPK, P-p38-MAPK, p44/42-MAPK, and P-p44/42 MAPK in siRNA-MIF-MCF-7 cells had different degree of decrease compared with siRNA-NC-MCF-7 cells and MCF-7 cells. Conclusions: These results suggest that MIF can regulate the expression of VEGF-C in breast cancer cells. And its regulatory mechanism may work by activating the MAPK signaling pathway. © 2016 Zhang et al.


PubMed | Guilin Medical University, Nanxishan Hospital of Guangxi Zhuang Autonomous Region and Soochow University of China
Type: | Journal: World journal of surgical oncology | Year: 2016

As a kind of versatility of cytokines, overexpression of macrophage migration inhibitory factor (MIF) and vascular endothelial growth factor-C (VEGF-C) have been reported in a wide variety of tumors. However, the correlation and mechanism between MIF and VEGF-C are still not clear. As an important signal transduction system, MAPK signaling pathways participate in a variety of biological behavior of cells. The purposes of this study are to study the relationship between MIF and VEGF-C and discuss the role of MAPK signal pathway in the relationship.In this study, we first knocked down the MIF using small interfering RNA (siRNA) and built the stable low expression MIF breast cancer cells (siRNA-MIF-MCF-7) and the negative control cells (siRNA-NC-MCF-7). And then, we evaluated the expression of MIF using Western blot to confirm the effect of transfection. Using real-time fluorescent quantitative polymerase chain reaction and enzyme-linked immunosorbent experiment, we respectively examined the different expression of VEGF-C between siRNA-MIF-MCF-7 and siRNA-NC-MCF-7 and breast cancer cells MCF-7. Moreover, we investigated the expression of p38 MAPK, P-p38 MAPK, p44/42 MAPK, and P-p44/42 MAPK in the three kinds of cells by Western blot to analyze the regulatory mechanism to VEGF-C.We found that MIF siRNA markedly reduced the expression of MIF. And the expression level of VEGF-C, p38 MAPK, P-p38-MAPK, p44/42-MAPK, and P-p44/42 MAPK in siRNA-MIF-MCF-7 cells had different degree of decrease compared with siRNA-NC-MCF-7 cells and MCF-7 cells.These results suggest that MIF can regulate the expression of VEGF-C in breast cancer cells. And its regulatory mechanism may work by activating the MAPK signaling pathway.


Li D.-Y.,Nanxishan Hospital of Guangxi Zhuang Autonomous Region
Chinese Critical Care Medicine | Year: 2010

Objective: To survey different diagnostic techniques in diagnosing pulmonary embolism (PE). Methods: Hospital records of PE cases in 13 AAA general hospitals in Guangxi area from 1995 to 2007 were studied retrospectively. Probable PE was defined as the diagnosis based on the clinical data and non-specific imaging, while the definite PE was defined as those with the diagnosis confirmed by specific imaging or autopsy. The percentage of various diagnostic methods of PE was analyzed. Results: From 1995 to 2007, 237 definite PE and 223 probable PE were found in 13 hospitals, and they accounted for 51.52% and 48.48%, respectively, for all patients diagnosed as having PE. The percentage of definite PE cases during 1995-2001 and 2002-2007 were 14.63% and 55.13%, respectively (X2 = 24.522, P<0.01). Among 237 definite PE, 2 positive diagnostic techniques were employed in 17 patients. Twenty-seven (11.39%), 214 (90.30%), 6 (2.53%), 5 (2.11%) and 2 (0.84%) patients were diagnosed by pulmonary angiography, CT pulmonary angiography (CTPA), ultrasonography, magnetic resonance imaging (MRI) and autopsy, respectively. No ventilation-perfusion lung scanning was performed in these patients. Compared with other diagnostic imaging, the percentage of CTPA in diagnosis of PE increased slightly since 2003. Conclusion: CTPA is the first choice in the diagnosis of PE in Guangxi area, and more attention should be paid to other diagnostic imaging techniques.


Lin Y.-H.,Nanxishan Hospital of Guangxi Zhuang Autonomous Region | Zhao Z.-Y.,Jinan University
International Journal of Ophthalmology | Year: 2010

• AIM: To investigate the inhibiting effect of intravitreal defibrase and vancomycin injection on proliferative vitreoretinopathy (PVR), infectious endophthalmitis in rabbit model eyes of hemorrhagic penetrating ocular injury. • METHODS: Totally 40 New Zealand albino rabbits (right eyes) were taken as experimental rabbit model eyes of hemorrhagic penetrating ocular injury and all their left eyes were intact. The rabbits were divided into four groups randomly: phosphate buffered saline group (10 eyes) which were injected with 0.1mL phosphate buffered saline in vitreous; defibrase group (10 eyes) which were injected with 0.1U(0.1mL) defibrase in vitreous; vancomycin group(10 eyes) which were injected with 1mg (0.1mL) vancomycin in vitreous; defibrase combined with vancomycin group (10 eyes) which were injected with 0.1U(0.1mL) defibrase and 1mg (0.1mL) vancomycin in vitreous respectively. The inflammation in anterior segment of eye was estimated by slit-lamp microscope examination. Microbiology examination in vitreous must be taken upon these eyes that the inflammation have last over two weeks in anterior segment. Both degree of PVR and the bleeding indexes in vitreous were estimated by direct funduscopy examination. • RESULTS: Both the bleeding indexes and degree of PVR in vitreous in defibrase combined with vancomycin group were lower than both phosphate buffered saline group (P < 0.01; P < 0.01) and vancomycin group(P < 0.05; P < 0.01); There were 3 eyes (30%) suffered endophthalmitis in both phosphate buffered saline group and defibrase group respectively; There was no eye suffered endophthalmitis in both vancomycin group and defibrase combined with vancomycin group. • CONCLUSION: The therapeutics of intravitreal defibrase combined with vancomycin injection can promotes the absorption of vitreous hemorrhage and decrease both the occurrence of bacterial endophthalmitis and the degree of PVR .


PubMed | Nanxishan Hospital of Guangxi Zhuang Autonomous Region
Type: Journal Article | Journal: Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue | Year: 2010

To survey different diagnostic techniques in diagnosing pulmonary embolism (PE).Hospital records of PE cases in 13 AAA general hospitals in Guangxi area from 1995 to 2007 were studied retrospectively. Probable PE was defined as the diagnosis based on the clinical data and non-specific imaging, while the definite PE was defined as those with the diagnosis confirmed by specific imaging or autopsy. The percentage of various diagnostic methods of PE was analyzed.From 1995 to 2007, 237 definite PE and 223 probable PE were found in 13 hospitals, and they accounted for 51.52% and 48.48%, respectively, for all patients diagnosed as having PE. The percentage of definite PE cases during 1995-2001 and 2002-2007 were 14.63% and 55.13%, respectively (chi (2)=24.522, P<0.01). Among 237 definite PE, 2 positive diagnostic techniques were employed in 17 patients. Twenty-seven (11.39%), 214 (90.30%), 6 (2.53%), 5 (2.11%) and 2 (0.84%) patients were diagnosed by pulmonary angiography, CT pulmonary angiography (CTPA), ultrasonography, magnetic resonance imaging (MRI) and autopsy, respectively. No ventilation-perfusion lung scanning was performed in these patients. Compared with other diagnostic imaging, the percentage of CTPA in diagnosis of PE increased slightly since 2003.CTPA is the first choice in the diagnosis of PE in Guangxi area, and more attention should be paid to other diagnostic imaging techniques.

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