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Li S.,Guilin Medical University | Liu T.,Nanxishan Hospital of Guangxi Zhuang Autonomous Region | Mo W.,Guilin Medical University | Hou Q.,Guilin Medical University | And 12 more authors.
Radiation Oncology | Year: 2016

Background: Radiation is an effective treatment against nasopharyngeal carcinoma (NPC). However, radioresistance-induced locoregional recurrence remains as a major cause of treatment failure. Therefore, radiosensitivity indicators prior to treatment should be developed to screen radioresistant patients. Previous studies revealed that RKIP (Raf kinase inhibitor protein) is associated with NPC prognosis and radiosensitivity. However, the relationship of p-Ser153 RKIP (RKIP in a phosphorylated form at residue serine153) expression with the effect of radiation and prognosis of NPC patients is not elucidated. Thus, these clinical implication of the phosphorylated RKIP in NPC has yet to be described. Methods: The effect of p-Ser153 RKIP on locoregional relapse-free survival (LRRFS) was first analyzed in a retrospective cohort of NPC patients without distant metastasis at initial diagnosis. They received radical intensity-modulated radiotherapy alone. Of 180 patients were enrolled in the ongoing matched pair study. The patients were re-classified into radioresistant group or radiosensitive group on the basis of the specified criteria. Patients in the two groups were matched in terms of radiosensitivity-related factors. p-Ser153 RKIP was examined by immunohistochemical staining on a NPC tissue microarray before radiotherapy. The relationship between the expression of p-Ser153 RKIP and the effect of radiotherapy was also analyzed. Results: In this study, a retrospective cohort with 733 cases who received radical radiotherapy alone was established. Using the cohort, we validated that the p-Ser153 RKIP expression observed through immunohistochemical staining in a pretreatment NPC tissue microarray was an independent prognostic factor of LRRFS and OS; we also confirmed that endemic patients with a positive p-Ser153 RKIP expression benefited from irradiation alone in terms of locoregional relapse-free survival. A total of 180 patients were enrolled in a matched pair study. Both groups were well matched in terms of radiosensitivity-related factors. On the basis of the p-Ser153 RKIP expression, we predicted the following data: 80.0 % sensitivity, 73.3 % specificity, 76.7 % accuracy, 75.0 % positive predictive value, and 78.6 % negative predictive value. Conclusions: Our results revealed for the first time that positive p-Ser153 RKIP expression was a favorable prognostic factor. It was also positively correlated with the radiosensitivity of NPC. p-Ser153 RKIP could also be used as a biomolecular marker with good availability and authenticity to preliminarily screen NPC-related clinical radiosensitivity. © 2016 The Author(s).


Hong Y.,Anhui Medical University | Hong Y.,Nanxishan Hospital of Guangxi Zhuang Autonomous Region | Qiao J.-L.,Nanxishan Hospital of Guangxi Zhuang Autonomous Region | Qiao J.-L.,Xingtai Peoples Hospital Of Hebei Province | And 6 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2016

Purpose: Understanding the difference of expression of FAS between breast cancer and benign lesion tissues of breast and discuss the expression of FAS in tissues of breast cancer and its clinical value. Methods: FAS mRNA and protein expression were determined by quantitative real-time reverse transcriptase-polymerase chain reaction and Western blotting in 20 pairs of fresh frozen breast cancer tissues, corresponding noncancerous tissues and normal tissues. Additionally, FAS expression was analyzed by immunohistochemistry in 108 cases of clinicopathologically characterized breast cancer patients. The correlation of FAS expression with patients’ survival rate was assessed by Kaplan-Meier. Results: The result shows that expression of FAS in mRNA and protein in the breast benign lesion is low level while in the noncancerous tissues is high, but in the tissues of breast cancer is revealed as excessive expression. FAS expression positive rate and staining intensity within the cancer tissues is higher than that of breast lesions (P<0.01). The high expression of FAS is significantly correlated with the diameter of tumor of the breast cancer (P=0.014) and transferring quantity of the surrounding lymph node (P=0.047). There is no statistical correlation found between FAS and ages of patients, menopause or not, tumor tissue ER, PR, c-erbB-2 (P>0.05). Kaplan-Meier survival analysis showed that a high expression level of FAS resulted in a significantly poor prognosis of breast cancer patients. Conclusion: In conclusion, overexpression of FAS is closely related to progression of breast cancer and might be regarded as an independent predictor of poor prognosis for breast cancer. © 2016, E-Century Publishing Corporation. All rights reserved.


Liu H.-H.,Guangxi Medical University | Liu H.-H.,Nanxishan Hospital of Guangxi Zhuang Autonomous Region | Wang J.,Nanxishan Hospital of Guangxi Zhuang Autonomous Region | Chen X.-M.,Guangxi Medical University | And 3 more authors.
Medicine (United States) | Year: 2016

In the present study, we adopted a novel method - local diffusion homogeneity (LDH) - to characterize the structure feature in mesial temporal lobe epilepsy (MTLE). Diffusion-weighted images were acquired from 11 left MTLE patients, 16 right MTLE patients, and 20 healthy controls from May 2014 to January 2015. Local diffusion homogeneity was compared among patient groups and controls by 2 sample t test. The discriminative value of LDH abnormalities was examined by receiver operating characteristic (ROC) curve analysis. Correlations with disease duration and onset age in both patient groups were assessed using Pearson's coefficient. Both patient groups exhibited lower LDH in the anterior corpus callosum (P < 0.05, corrected), and this regional anomaly exhibited excellent classification performance in left MTLE patients (sensitivity = 82%, specificity = 100%), right MTLE patients (sensitivity = 81%, specificity = 90%), and the entire patient cohort (sensitivity = 82%, specificity = 95%). In summary, left and right MTLE patients show common pathological changes in the anterior corpus callosum. This regional LDH abnormality is a potential quantitative biomarker for MTLE. Copyright © 2016 the Author(s). Published by Wolters Kluwer Health, Inc. All rights reserved.


Niu G.-J.,Guangxi Medical University | Niu G.-J.,Nanxishan Hospital of Guangxi Zhuang Autonomous Region | Tang G.-D.,Guangxi Medical University | Liang Z.-H.,Guangxi Medical University | And 2 more authors.
World Chinese Journal of Digestology | Year: 2010

AIM: To investigate the expression of thioredoxin-1 (Trx-1) in the lungs of rats with L-arginine (L-Arg)-induced acute necrotizing pancreatitis (ANP) and assess the effects of melatonin on Trx-1 expression. METHODS: Seventy-two male Sprague-Dawley rats were randomly divided into three groups: normal control group, model control group and melatonin intervention group. The ANP model group was intraperitoneally injected three times with 6% L-Arg at a dose of 25 mL/kg body weight at an interval of 1 h to induce ANP. The normal control group was intraperitoneally injected with equal volumes of normal saline. The melatonin intervention group was injected intraperitoneally with 0.25% melatonin at a dose of 20 mL/kg body weight half an hour before ANP induction. Rats were executed at 6, 12 and 24 hours after last L-Arg injection. The expression of Trx-1 in the lungs was detected by immunohistochemistry. The pathological changes in the pancreas and lungs were analyzed and scored according to Kusser's and Lei's criteria, respectively. The contents of serum Trx-1 and amylase were measured. RESULTS: At 6, 12 and 24 hours after last L-Arg injection, the pathological changes in the pancreas and lungs in the model control group were more severe than those in the normal control group (all P < 0.01). However, the pathological changes in the pancreas and lungs in the melatonin intervention group were milder than those in the model control group (P < 0.01 or 0.05). At 24 hours, the content of serum amylase in the model control group was significantly higher than that in the normal control group (4 598 U/L ± 2 274 U/L vs 2 033 U/L ± 863 U/L, P < 0.01). In contrast, the content of serum amylase in the melatonin intervention group was lower than that in the model control group (3 990 U/L ± 1 146 U/L vs 4 598 U/L ± 2 274 U/L, P < 0.05). Compared to the normal control group, serum Trx-1 contents in the model control group significantly decreased at 6 and 12 hours but significantly increased at 24 hours. The contents of serum Trx-1 in melatonin intervention group at 6 and 12 hours were significantly higher than those in the model control group. CONCLUSION: Lung injury is closely related to pancreatic injury in ANP. The expression of Trx-1 in the lungs of rats with ANP increases significantly. Overexpression of Trx-1 in the lungs is closely associated with the development of ANP and acute pancreatitis-associated lung injury. Melatonin can, to a certain extent, alter the expression of Trx-1 and reduce pancreatic and pulmonary injury in ANP in rats.


Zhang J.,Guilin Medical University | Zhang G.,Soochow University of China | Yang S.,Guilin Medical University | Qiao J.,Guilin Medical University | And 2 more authors.
World Journal of Surgical Oncology | Year: 2016

Background: As a kind of versatility of cytokines, overexpression of macrophage migration inhibitory factor (MIF) and vascular endothelial growth factor-C (VEGF-C) have been reported in a wide variety of tumors. However, the correlation and mechanism between MIF and VEGF-C are still not clear. As an important signal transduction system, MAPK signaling pathways participate in a variety of biological behavior of cells. The purposes of this study are to study the relationship between MIF and VEGF-C and discuss the role of MAPK signal pathway in the relationship. Methods: In this study, we first knocked down the MIF using small interfering RNA (siRNA) and built the stable low expression MIF breast cancer cells (siRNA-MIF-MCF-7) and the negative control cells (siRNA-NC-MCF-7). And then, we evaluated the expression of MIF using Western blot to confirm the effect of transfection. Using real-time fluorescent quantitative polymerase chain reaction and enzyme-linked immunosorbent experiment, we respectively examined the different expression of VEGF-C between siRNA-MIF-MCF-7 and siRNA-NC-MCF-7 and breast cancer cells MCF-7. Moreover, we investigated the expression of p38 MAPK, P-p38 MAPK, p44/42 MAPK, and P-p44/42 MAPK in the three kinds of cells by Western blot to analyze the regulatory mechanism to VEGF-C. Results: We found that MIF siRNA markedly reduced the expression of MIF. And the expression level of VEGF-C, p38 MAPK, P-p38-MAPK, p44/42-MAPK, and P-p44/42 MAPK in siRNA-MIF-MCF-7 cells had different degree of decrease compared with siRNA-NC-MCF-7 cells and MCF-7 cells. Conclusions: These results suggest that MIF can regulate the expression of VEGF-C in breast cancer cells. And its regulatory mechanism may work by activating the MAPK signaling pathway. © 2016 Zhang et al.


PubMed | Guilin Medical University, Nanxishan Hospital of Guangxi Zhuang Autonomous Region and Soochow University of China
Type: | Journal: World journal of surgical oncology | Year: 2016

As a kind of versatility of cytokines, overexpression of macrophage migration inhibitory factor (MIF) and vascular endothelial growth factor-C (VEGF-C) have been reported in a wide variety of tumors. However, the correlation and mechanism between MIF and VEGF-C are still not clear. As an important signal transduction system, MAPK signaling pathways participate in a variety of biological behavior of cells. The purposes of this study are to study the relationship between MIF and VEGF-C and discuss the role of MAPK signal pathway in the relationship.In this study, we first knocked down the MIF using small interfering RNA (siRNA) and built the stable low expression MIF breast cancer cells (siRNA-MIF-MCF-7) and the negative control cells (siRNA-NC-MCF-7). And then, we evaluated the expression of MIF using Western blot to confirm the effect of transfection. Using real-time fluorescent quantitative polymerase chain reaction and enzyme-linked immunosorbent experiment, we respectively examined the different expression of VEGF-C between siRNA-MIF-MCF-7 and siRNA-NC-MCF-7 and breast cancer cells MCF-7. Moreover, we investigated the expression of p38 MAPK, P-p38 MAPK, p44/42 MAPK, and P-p44/42 MAPK in the three kinds of cells by Western blot to analyze the regulatory mechanism to VEGF-C.We found that MIF siRNA markedly reduced the expression of MIF. And the expression level of VEGF-C, p38 MAPK, P-p38-MAPK, p44/42-MAPK, and P-p44/42 MAPK in siRNA-MIF-MCF-7 cells had different degree of decrease compared with siRNA-NC-MCF-7 cells and MCF-7 cells.These results suggest that MIF can regulate the expression of VEGF-C in breast cancer cells. And its regulatory mechanism may work by activating the MAPK signaling pathway.


Li D.-Y.,Nanxishan Hospital of Guangxi Zhuang Autonomous Region
Chinese Critical Care Medicine | Year: 2010

Objective: To survey different diagnostic techniques in diagnosing pulmonary embolism (PE). Methods: Hospital records of PE cases in 13 AAA general hospitals in Guangxi area from 1995 to 2007 were studied retrospectively. Probable PE was defined as the diagnosis based on the clinical data and non-specific imaging, while the definite PE was defined as those with the diagnosis confirmed by specific imaging or autopsy. The percentage of various diagnostic methods of PE was analyzed. Results: From 1995 to 2007, 237 definite PE and 223 probable PE were found in 13 hospitals, and they accounted for 51.52% and 48.48%, respectively, for all patients diagnosed as having PE. The percentage of definite PE cases during 1995-2001 and 2002-2007 were 14.63% and 55.13%, respectively (X2 = 24.522, P<0.01). Among 237 definite PE, 2 positive diagnostic techniques were employed in 17 patients. Twenty-seven (11.39%), 214 (90.30%), 6 (2.53%), 5 (2.11%) and 2 (0.84%) patients were diagnosed by pulmonary angiography, CT pulmonary angiography (CTPA), ultrasonography, magnetic resonance imaging (MRI) and autopsy, respectively. No ventilation-perfusion lung scanning was performed in these patients. Compared with other diagnostic imaging, the percentage of CTPA in diagnosis of PE increased slightly since 2003. Conclusion: CTPA is the first choice in the diagnosis of PE in Guangxi area, and more attention should be paid to other diagnostic imaging techniques.


Lin Y.-H.,Nanxishan Hospital of Guangxi Zhuang Autonomous Region | Zhao Z.-Y.,Jinan University
International Journal of Ophthalmology | Year: 2010

• AIM: To investigate the inhibiting effect of intravitreal defibrase and vancomycin injection on proliferative vitreoretinopathy (PVR), infectious endophthalmitis in rabbit model eyes of hemorrhagic penetrating ocular injury. • METHODS: Totally 40 New Zealand albino rabbits (right eyes) were taken as experimental rabbit model eyes of hemorrhagic penetrating ocular injury and all their left eyes were intact. The rabbits were divided into four groups randomly: phosphate buffered saline group (10 eyes) which were injected with 0.1mL phosphate buffered saline in vitreous; defibrase group (10 eyes) which were injected with 0.1U(0.1mL) defibrase in vitreous; vancomycin group(10 eyes) which were injected with 1mg (0.1mL) vancomycin in vitreous; defibrase combined with vancomycin group (10 eyes) which were injected with 0.1U(0.1mL) defibrase and 1mg (0.1mL) vancomycin in vitreous respectively. The inflammation in anterior segment of eye was estimated by slit-lamp microscope examination. Microbiology examination in vitreous must be taken upon these eyes that the inflammation have last over two weeks in anterior segment. Both degree of PVR and the bleeding indexes in vitreous were estimated by direct funduscopy examination. • RESULTS: Both the bleeding indexes and degree of PVR in vitreous in defibrase combined with vancomycin group were lower than both phosphate buffered saline group (P < 0.01; P < 0.01) and vancomycin group(P < 0.05; P < 0.01); There were 3 eyes (30%) suffered endophthalmitis in both phosphate buffered saline group and defibrase group respectively; There was no eye suffered endophthalmitis in both vancomycin group and defibrase combined with vancomycin group. • CONCLUSION: The therapeutics of intravitreal defibrase combined with vancomycin injection can promotes the absorption of vitreous hemorrhage and decrease both the occurrence of bacterial endophthalmitis and the degree of PVR .


PubMed | Nanxishan Hospital of Guangxi Zhuang Autonomous Region
Type: Journal Article | Journal: Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue | Year: 2010

To survey different diagnostic techniques in diagnosing pulmonary embolism (PE).Hospital records of PE cases in 13 AAA general hospitals in Guangxi area from 1995 to 2007 were studied retrospectively. Probable PE was defined as the diagnosis based on the clinical data and non-specific imaging, while the definite PE was defined as those with the diagnosis confirmed by specific imaging or autopsy. The percentage of various diagnostic methods of PE was analyzed.From 1995 to 2007, 237 definite PE and 223 probable PE were found in 13 hospitals, and they accounted for 51.52% and 48.48%, respectively, for all patients diagnosed as having PE. The percentage of definite PE cases during 1995-2001 and 2002-2007 were 14.63% and 55.13%, respectively (chi (2)=24.522, P<0.01). Among 237 definite PE, 2 positive diagnostic techniques were employed in 17 patients. Twenty-seven (11.39%), 214 (90.30%), 6 (2.53%), 5 (2.11%) and 2 (0.84%) patients were diagnosed by pulmonary angiography, CT pulmonary angiography (CTPA), ultrasonography, magnetic resonance imaging (MRI) and autopsy, respectively. No ventilation-perfusion lung scanning was performed in these patients. Compared with other diagnostic imaging, the percentage of CTPA in diagnosis of PE increased slightly since 2003.CTPA is the first choice in the diagnosis of PE in Guangxi area, and more attention should be paid to other diagnostic imaging techniques.

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