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Guo P.,Guangzhou University | Xia Y.,Guangzhou University | Li R.,Guangzhou University | Yonghua L.,Guangzhou University | And 3 more authors.
Advanced Materials Research | Year: 2013

The tobacco-specific nitrosamines (TSNAs) are one of the most important groups of carcinoigens in tobacco leaf, knowing the difference of TSNAs contents in tobacco genotypes should be beneficial for breeding low TSNAs cultivars which could decrease the harmfulness to human health in tobacco products. For this purpose, 8 genotypes (include burley, sun-cured and flue-cured tobacco) were selected as materials, the TSNAs contents of uncured and cured leaves were assessed with LC-MS/MS. The results showed that TSNAs contents were significantly increased after curing in all genotypes. The mean TSNAs contents of leaves before and after curing in burley were the highest among three types of tobacco, and the contents in sun-cured tobacco were the lowest. Coefficient of variation (CV) of TSNAs among genotypes in burley was higher than that in flue-cured and sun-cured tobacco, and the CV in sun-cured tobacco was the lowest. The positive correlation was observed in NNN, NAT and total TSNAs contents between uncured and cured leaves in three types of tobacco. Therefore, the TSNAs contents in uncured leaves could be considered as evaluation index to screen tobacco cultivars with the low TSNAs contents. © (2013) Trans Tech Publications, Switzerland.


Yu Y.,Guangzhou University | Xia Y.,Guangzhou University | Li R.,Guangzhou University | Lu Y.,Guangdong Provincial Tobacco Monopoly Administration | And 4 more authors.
Acta Tabacaria Sinica | Year: 2014

Genetic diversity analysis for 24 tobacco accessions was performed with SSR and MFLP markers which distributed in 24 chromosomes. Association analysis was conducted between polymorphic markers and tobacco specific nitrosamine (TSNA) contents through GLM model using software TASSEL. Result showed that 188 allelic variations were detected among 24 tobacco accessions with 33 MFLP and 28 SSR primer combinations. These accessions were clustered into 3 subgroups in genetic analysis of population structure. The subgroup is in coincidence with tobacco types, i.e. flue-cured, sun-cured and burley. Association analysis showed that 6 MFLP markers and 1 SSR marker were associated with at least one of TSNA contents at P<0.01 level. The marker of MFLP26 was significantly associated with NNN content (P<0.001), and explains 58.31% of phenotypic variation for NNN content in cured leaves. These markers could be used in tobacco breeding programmes to assist with selection of tobacco genotypes with low TSNA content.


Xia Y.S.,Guangzhou University | Guo P.G.,Guangzhou University | Li R.H.,Guangzhou University | Lu Y.H.,Guangdong Tobacco Monopoly Administration | And 5 more authors.
Advanced Materials Research | Year: 2014

Genetic diversity analysis and population structure can estimate genetic variation of diverse materials, and can be used in germplasm management and varietal protection. In this study, the genetic diversity and population structure for tobacco germplasm (78 cultivated tobacco accessions in China) were analyzed by using 28 SSR markers. A total of 127 alleles were detected with an average of 4.5 per locus in 78 accessions, while PIC values ranged from 0.19 to 0.89 with an average of 0.63 per marker. Based on genetic similarity, most of sun-cured and flue-cured tobacco accessions were clustered into group A and group B, respectively. A model-based structure analysis for these accessions detected two subpopulations, which were generally coincident with the clustering analysis and showed the genetic similarities were relative high for these accessions. These results revealed narrow genetic diversity for the tobacco germplasm in China. © (2014) Trans Tech Publications, Switzerland.

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