Nantong Products Quality Supervision and Inspection Institute

Nantong, China

Nantong Products Quality Supervision and Inspection Institute

Nantong, China
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Wang S.,Jiangnan University | Wang S.,Fuzhou University | Shao B.,Nantong Products Quality Supervision and Inspection Institute | Lu W.,Fuzhou University | And 3 more authors.
Preparative Biochemistry and Biotechnology | Year: 2014

A novel trypsin inhibitor with thermal and pH stability, designated Merrtine, was isolated from Glycine max L. merr. The procedure involved ammonium sulfate precipitation, ion-exchange chromatography on CM-Sephadex C-50, and affinity chromatography on Affi-gel blue gel. The 20 N-terminal amino acid sequences were determined to be DEYSKPCCDLCMCTRRCPPQ, demonstrating high homology with the sequence of Bowman-Birk type trypsin inhibitors. The molecular mass and isoelectric point of the inhibitor were estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing to be 20.0 kD and 5.8, respectively. Trypsin could be completely inhibited by Merrtine when the molar ratio was 8.1. The inhibitory activity of Merrtine was unaffected after exposure to temperatures up to 85°C, as well as within the pH range 2-12. Besides inhibiting trypsin-chymotrypsin, the inhibitor demonstrated additional antifungal activity against the species of Alternaria alternate, Fusarium oxysporum, Pythium aphanidermatum, Physalospora piricola, Botrytis cinerea, and Fusarium solani. We herein report not only the trypsin inhibitor's extraction and isolation for the first time, but also its physiochemical and antifungal properties. © 2014 Copyright Taylor and Francis Group, LLC.


Hong Y.,Fuzhou University | Cai X.,Fuzhou University | Shao B.,Nantong Products Quality Supervision and Inspection Institute | Hong J.,Fuzhou University | And 2 more authors.
European Food Research and Technology | Year: 2013

A novel trypsin inhibitor with considerable thermal and pH stability, designated Glytine, was isolated from seeds of the Chinese black soybean Glycine max (L.) Merr. The purification procedure involved ammonium sulfate precipitation, ion-exchange chromatography on CM-Sephadex C-50, gel filtration chromatography on Sephacryl S-200HR, and gel filtration chromatography on POROS HS-20. The 20 N-terminal amino acid sequences were determined to be DEYSKPCCDLCMCTRRCPPQ, demonstrating close homology with the sequences of leguminous trypsin inhibitors. The molecular mass and isoelectric point of the inhibitor were estimated by SDS-PAGE and isoelectric focusing to be 19.9 kDa and 6.2, respectively. Trypsin could be completely inhibited by Glytine when the weight ratio was 1.5. The inhibitory activity of Glytine was unaffected by exposure to temperatures up to 100 °C, or within the pH range 2-12. Besides trypsin-chymotrypsin inhibition activity, Glytine demonstrated other biological activities including antiproliferative activity against tumor cells including human liver hepatoma cells Bel-7402 and neuroblastoma cells SHSY5Y. In addition, the inhibitor showed antifungal activity against Pythium aphanidermatum, Fusarium oxysporum, Alternaria alternata (Fr.) Keiss, Fusarium solani, and Botrytis cinerea. This study extended research on leguminous trypsin-chymotrypsin inhibitor and suggested exploitable potential. © 2013 Springer-Verlag Berlin Heidelberg.


Zhang W.,Changzhou University | Luo X.,Changzhou University | Luo X.,Nantong Products Quality Supervision and Inspection Institute | Han Y.,Changzhou University | And 2 more authors.
Tumu Jianzhu yu Huanjing Gongcheng/Journal of Civil, Architectural and Environmental Engineering | Year: 2013

When the salvaged cyanobacteria was dehydrated by centrifugation, the algae pulp filtrate produced. And there were a large number of algae cell sap, nitrogen, phosphorus, and algae toxin. If it is discharged into water directly, it would cause secondary pollution. The downward-flow biological aerated filter was used to treat the algae pulp filtrate, and the removal effect of COD, NH3-N, TN, TP and MC-LR was investigated. The result showed that the average removal rate of COD, NH3-N, TN, TP and MC-LR were 73.4%, 91.6%, 39.6%, 13.2% and 88.3% respectively under the condition that the hydraulic loading was for 0.40 m3/(m2·h). The COD and NH3-N meet standard A of the first class in "Discharge standard of pollutants for municipal wastewater treatment plant" (GB18918-2002), and the concentration of MC-LR is lower than the limit of "Standards for Drinking Water Quality"(GB 5749-2006). The microbial species in biological aerated filter was analyzed. The dominant bacteria were Lysinibacillus, aeromonas, bacillus, acinetobacter, achromobacter, bacillus, fusiformis, and pseudomonas respectively determined by constructing phylogenetic tree. The cyanobacteria toxin degradation bacterium of T1 which was put in biological aerated filter in start-up grew well and form certain advantages.

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