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Jin W.,CAS Qingdao Institute of Oceanology | Jin W.,University of Chinese Academy of Sciences | Jin W.,Nantong Marine Science and Technology Randnter | Wang J.,CAS Qingdao Institute of Oceanology | And 7 more authors.
Carbohydrate Polymers | Year: 2013

Crude fucoidan extracted from Saccharina japonica was separated by anion-exchange chromatography. Then, the neuroprotective activities of the crude fucoidan (J) and its fractions (J0.4, J0.5, J1 and J2) were tested. J, J0.4 and J0.5 were shown to have neuroprotective effects. To simplify the structural features of the compounds, crude fucoidan was degraded to obtain low molecular weight fucoidan (DJ). DJ was further fractionated into DJ0.5, DJ1 and DJ2, and the neuroprotective activities of these fractions were determined. This analysis revealed that DJ and DJ0.5 retained the neuroprotective activity. However, the DJ0.5 fraction remained very complex. Thus, DJ0.5 was further separated into six fractions (F0.1, F0.2, F0.3, F0.4, F0.5 and F1). Finally, it was concluded that the anion-exchange fractions F0.1, F0.2 and F0.3 exhibited neuroprotective activities. These results suggest that heteropolysaccharides might contribute to the neuroprotective activity. Moreover, the neuroprotective heteropolysaccharide fractions contained relatively low fucose (less than 20%) and sulfate (25%), high UA content (more than 10%) and a high molar ratio of all other monosaccharides © 2013 Elsevier Ltd. All rights reserved.


Jin W.,CAS Qingdao Institute of Oceanology | Jin W.,University of Chinese Academy of Sciences | Zhang Q.,CAS Qingdao Institute of Oceanology | Wang J.,CAS Qingdao Institute of Oceanology | And 3 more authors.
Carbohydrate Polymers | Year: 2013

Seven fucoidans that differed only with respect to the average molecular weight and four fucoidans that differed with respect to both the molar ratio of fucose to galactose and the average molecular weight were obtained. The anticoagulant activities of these fucoidans were determined in order to understand the effects of the average molecular weight and the content of galactose. Fucoidans Y5-Y11, with the average molecular weight in the range of 50.1-8.4 kDa, showed decreasing activity at three concentrations and dose-dependent activity in the APTT and TT assays. Y1-Y4, which differed with respect to the average molecular weight and the molar ratio of fucose to galactose, exhibited complicated results in the APTT and TT assays. It was shown that not only the average molecular weight but also the molar ratio of fucose to galactose affected the anticoagulant activity of fucoidans. © 2012 Elsevier Ltd.


Jin W.,CAS Qingdao Institute of Oceanology | Jin W.,University of Chinese Academy of Sciences | Jin W.,Nantong Marine Science and Technology Randnter | Guo Z.,CAS Dalian Institute of Chemical Physics | And 5 more authors.
Carbohydrate Research | Year: 2013

Desulfation of a fucoidan from Saccharina japonica by treatment with DMSO-MeOH resulted in partial degradation of polymeric molecules by methanolysis giving rise to a mixture of neutral, monosulfated, and disulfated fucooligosaccharides in the form of methyl glycosides. These oligomeric fragments were characterized by ESI-MS and ESI-CID-MS/MS. It was found that oligosaccharide structures coincided with the polysaccharide backbone built up mainly of (1→3)-linked fucose residues sulfated at positions 4 and 2. © 2013 Elsevier Inc. All rights reserved.

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