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Wang S.,Nantong University | Zhang R.-P.,Nantong University | Zhang R.-P.,Donghua University | Shen X.-P.,Nantong Entry exit Inspection and Quarantine Bureau | And 2 more authors.
Wool Textile Journal

Transglutaminases could catalyze an acyl transfer reaction between peptide-bound glutamine (acyl-donor) and lysine primary amine ( acyl-acceptor). The crosslinking of proteins via intra or inter ε-(γ-glutamyl) lysine isodipeptide bridges improved protein stability. Wool knitted fabrics were treated with transglutaminase in order to decrease the damage of wool resulting from dyeing and protease treatment. The prevention and remedy effect of transglutaminase on the damage of wool fabric were studied. The results revealed that the transglutaminase could prevent and remediate the damage caused by protease, the bursting strength of the knitted fabric treated with transglutaminase increased by 8.17% and 8.42% respectively. Transglutaminase could also prevent and remediate wool damage caused by dyeing, the bursting strength of the knitted fabric treated with transglutaminase increased by 8.2% and 17.0% respectively. The result of DTA suggested the thermal degradation temperature of wool treated with transglutaminase raised. Source

Song H.-Y.,Nantong University | Qiu B.-F.,Nantong Entry exit Inspection and Quarantine Bureau | Liu C.,Nantong University | Zhu S.-X.,Nantong University | And 4 more authors.
Experimental Animals

The clone library method using PCR amplification of the 16S ribosomal RNA (rRNA) gene was used to identify pathogens from corneal scrapings of C57BL/6-corneal opacity (B6-Co) mice with bacterial keratitis. All 10 samples from the eyes with bacterial keratitis showed positive PCR results. All 10 samples from the normal cornea showed negative PCR results. In all 10 PCR-positive samples, the predominant and second most predominant species accounted for 20.9 to 40.6% and 14.7 to 26.1%, respectively, of each clone library. The predominant species were Staphylococcus lentus, Pseudomonas aeruginosa, and Staphylococcus epidermidis. The microbiota analysis detected a diverse group of microbiota in the eyes of B6-Co mice with bacterial keratitis and showed that the causative pathogens could be determined based on percentages of bacterial species in the clone libraries. The bacterial species detected in this study were mostly in accordance with results of studies on clinical bacterial keratitis in human eyes. Based on the results of our previous studies and this study, the B6-Co mouse should be considered a favorable model for st udying bacterial keratitis. © 2015 Japanese Association for Laboratory Animal Science. Source

Sun J.,General Research Institute for Nonferrous Metals, China | Zhang Y.,General Research Institute for Nonferrous Metals, China | Huang C.,General Research Institute for Nonferrous Metals, China | Li Y.,General Research Institute for Nonferrous Metals, China | Li G.,Nantong Entry exit Inspection and Quarantine Bureau
Zhongguo Jiguang/Chinese Journal of Lasers

Wall-like Ti60 alloy and TiCP (mass fraction 5%)/Ti60 titanium matrix composites are fabricated by laser direct deposition process. Their microstructure and tensile properties under 600°C are investigated. The results indicate that the deposited Ti60 alloy presents a widmanstatten structure with some spherical rare earth phases distributing along the sub-grain boundaries and the α/β interface. For the deposited TiCP/Ti60 composites, there are some un-melted TiC particles and re-solidified TiC particles appear as discontinuous chain-like. The TiC particles distribute uniformly within the matrix with good interface bonding. Under 600°C, the ultimate tensile strength (UTS) of TiCP/Ti60 composites is 65 MPa higher than that of Ti60 alloy, following with decreased elongation. Ti60 alloy exhibit ductile fracture characteristics, while TiCP/Ti60 composite exhibit some complicated fracture characteristics including intergranular fracture, quasi-cleavage crack and local ductile rupture. Source

Wang Y.,Tongji University | Guo G.,Nantong Entry exit Inspection and Quarantine Bureau
Progress in Chemistry

1Asymmetric nanomaterials (Janus nanomaterials) comprising at least two components of different chemistry, functionality, and/or polarity have attracted great scientific interest in a wide range of applications. Additional properties are attributed to the asymmetric spatial distribution of functionalities on a single anisotropic nanoparticle, like amphiphilicity or new catalytic characteristic. In addition, Janus nanomaterials' synergistic potential for multilevel targeting, and combination therapies make them particularly attractive for biomedical applications including biosensing, targeting delivery and bio-detection. In this paper, the advances in properties and applications of Janus nanomaterials are summarized. First, some properties and applications of Janus nanomaterials are described in three different aspects: amphipathicity, catalytic characteristic and biocompatibility. Second, the main biomedical applications are highlighted, which include biosensing, targeting delivery, gene vaccine and antimicrobial. Finally, the further development in preparation of Janus nanomaterials and their applications in food safety are expected. ©, 2014, Chinese Academy of Sciences. All right reserved. Source

Dou H.-Z.,Nantong Entry exit Inspection and Quarantine Bureau | Lu C.-X.,Nantong Entry exit Inspection and Quarantine Bureau | Hou J.,Nantong Entry exit Inspection and Quarantine Bureau
Yejin Fenxi/Metallurgical Analysis

The simultaneous determination of fluoride and chloride contents in nickel ore by ion chromatography was established. The sample was decomposed by H 2SO 4. After water steam distillation at 160-180°C, the fluoride and chloride in testing solution would be released and absorbed by NaOH solution, realizing the separation from matrix and other elements. Then, the contents of fluoride and chloride were determined by ion chromatography. The high-capacity IonPac ® AS23 anion separation column (4 mm×250 mm) and IonPac ® AG23 protection column (4 mm×50 mm) were used as chromatographic column and 20 mmol/L NaOH solution was used as eluent. Fluoride and chloride were detected with conductivity detector. The results showed that the peak area of fluoride and chloride had good linear relationship with their contents in the range of 1-25 μg/mL. The correlation coefficients (R) were 1.0000 and 0.9996 for fluoride and chloride respectively. The determination results of fluoride and chloride in sample showed high precision. The recoveries of standard addition were all greater than 95%. Source

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