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Nicolini C.,Nanoworld Institute
Bioengineered | Year: 2013

This paper investigates the application of anodic porous alumina as an advancement on chip laboratory for gene expressions. The surface was prepared by a suitable electrolytic process to obtain a regular distribution of deep micrometric holes and printed bypen robot tips under standard conditions. The gene expression within the Nucleic Acid Programmable Protein Array (NAPPA) is realized in a confined environment of 16 spots, containing circular DNA plasmids expressed using rabbit reticulocyte lysate. Authors demonstrated the usefulness of APA in withholding the protein expression by detecting with a CCD microscope the photoluminescence signal emitted from the complex secondary antibody anchored to Cy3 and confined in the pores. Friction experiments proved the mechanical resistance under external stresses by the robot tip pens printing. So far, no attempts have been made to directly compare APA with any other surface/substrate; the rationale for pursuing APA as a potential surface coating is that it provides advantages over the simple functionalization of a glass slide, overcoming concerns about printing and its ability to generate viable arrays.


Nicolini C.,Nanoworld Institute
Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology | Year: 2010

This review presents the status of technological developments of nanogenomics and its applications to medicine. Even if particular emphasis is placed on what has been accomplished in our laboratory in the last few years in the area of genes microarrays, significant reference to the recent activity of numerous other groups can be found in Refs 1,2. © 2009 John Wiley & Sons, Inc.


Spera R.,University of Genoa | Correia T.T.B.,University of Genoa | Nicolini C.,Nanoworld Institute | Nicolini C.,University of Genoa | Nicolini C.,Arizona State University
Sensors and Actuators, B: Chemical | Year: 2013

We present our preliminary results about the implementation of a new nanogravimetric biosensor realized combining Quartz Crystal Microbalance with Dissipation Monitoring Nanogravimetry and an innovative protein cell-free expression system named Nucleic Acid Programmable Protein Arrays (NAPPA) that allowed us to immobilize on the quartz surface, as sensing molecule, the proteins starting from its plasmid. We herby present results about the characterization of the nanogravimetric biosensor, its sensitivity, and its selectivity. Moreover we demonstrated how the simultaneous measurements of changes in resonance frequency and in energy dissipation were fundamental to correctly interpret the behavior of complex system such as NAPPA. © 2013 Elsevier B.V.


Pechkova E.,Nanoworld Institute | Nicolini C.,Fondazione EL.B.A.
Journal of Synchrotron Radiation | Year: 2011

Ultrasmall lysozyme microcrystals are grown by classical hanging-drop vapor diffusion and by its modification using a homologous protein thin-film template displaying long-range order. The nucleation and growth mechanisms of lysozyme microcrystals are studied at the thin lysozyme film surface using a new in situ μGISAXS (microbeam grazing-incidence small-angle X-ray scattering) technique recently developed at the microfocus beamline of the ESRF in Grenoble, France. New insight on the nucleation and crystallization processes appear to emerge. © 2011 International Union of Crystallography.


Gebhardt R.,European Synchrotron Radiation Facility | Gebhardt R.,TU Munich | Pechkova E.,Nanoworld Institute | Riekel C.,European Synchrotron Radiation Facility | Nicolini C.,Nanoworld Institute
Biophysical Journal | Year: 2010

The formation of thaumatin crystals by Langmuir-Blodgett (LB) film nanotemplates was studied by the hangingdrop technique in a flow-through cell by synchrotron radiation micrograzing-incidence small-angle x-ray scattering. The kinetics of crystallization was measured directly on the interface of the LB film crystallization nanotemplate. The evolution of the micro-grazing-incidence small-angle x-ray scattering patterns suggests that the increase in intensity in the Yoneda region is due to protein incorporation into the LB film. The intensity variation suggests several steps, which were modeled by system dynamics based on first-order differential equations. The kinetic data can be described by two processes that take place on the LB film, a first, fast, process, attributed to the crystal growth and its detachment from the LB film, and a second, slower process, attributed to an unordered association and conversion of protein on the LB film. © 2010 by the Biophysical Society.

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