NanoTemper Technologies GmbH

München, Germany

NanoTemper Technologies GmbH

München, Germany
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Patent
Nano Temper Technologies GmbH | Date: 2016-09-01

The present invention relates to a method and an apparatus for a fast thermo-optical characterisation of particles. In particular, the present invention relates to a method and a device to measure the stability of (bio)molecules, the interaction of molecules, in particular biomolecules, with, e.g. further (bio)molecules, particularly modified (bio)molecules, particles, beads, and/or the determination of the length/size (e.g. hydrodynamic radius) of individual (bio)molecules, particles, beads and/or the determination of length/size (e.g. hydrodynamic radius).


Patent
NanoTemper Technologies GmbH | Date: 2017-06-21

The present invention relates to a method for measuring the stability, for example based on the detected unfolding, of a biomolecule, such as a protein, peptide, antibody or nucleic acid, in a solution comprising:(a) providing a sample with the biomolecule in a solution;(b) exciting fluorescently the biomolecule and detecting a first fluorescence of the excited biomolecule;(c) irradiating a laser light beam, preferably a focused infrared laser light beam, into the solution to obtain a spatial temperature distribution in the solution around the irradiated laser light beam;(d) detecting a second fluorescence of the biomolecule in the solution at a predetermined time after irradiation of the laser into the solution has been started, and(e) characterizing the stability of the biomolecule based on the first and second detections.


Baaske P.,Ludwig Maximilians University of Munich | Baaske P.,NanoTemper Technologies GmbH | Wienken C.J.,Ludwig Maximilians University of Munich | Reineck P.,Ludwig Maximilians University of Munich | And 2 more authors.
Angewandte Chemie - International Edition | Year: 2010

"Chemical Equation Presented" Some like it hot: A robust and fast method for characterizing aptamers relies on the distinct thermophoretic movements of molecules in microscopic temperature gradients (see diagram). The binding properties of proteins and even small molecules can be measured within seconds, and less than 1 μL of sample is required. Notably, the technique works well in complex liquids such as human serum. © 2010 Wiley-VCH Verlag GmbH & Co. KCaA.


Wienken C.J.,Ludwig Maximilians University of Munich | Baaske P.,Ludwig Maximilians University of Munich | Baaske P.,NanoTemper Technologies GmbH | Rothbauer U.,Ludwig Maximilians University of Munich | And 3 more authors.
Nature Communications | Year: 2010

Protein interactions inside the human body are expected to differ from the situation in vitro. This is crucial when investigating protein functions or developing new drugs. In this study, we present a sample-efficient, free-solution method, termed microscale thermophoresis, that is capable of analysing interactions of proteins or small molecules in biological liquids such as blood serum or cell lysate. The technique is based on the thermophoresis of molecules, which provides information about molecule size, charge and hydration shell. We validated the method using immunologically relevant systems including human interferon gamma and the interaction of calmodulin with calcium. The affinity of the small-molecule inhibitor quercetin to its kinase PKA was determined in buffer and human serum, revealing a 400-fold reduced affinity in serum. This information about the influence of the biological matrix may allow to make more reliable conclusions on protein functionality, and may facilitate more efficient drug development. © 2010 Macmillan Publishers Limited. All rights reserved.


Patent
NanoTemper Technologies GmbH | Date: 2014-03-27

The invention generally relates to an apparatus and a method for mixing of liquids (50) or of particles with a liquid (50). In a volume of liquid (50), a thermal convection flow is generated at at least one surface of the volume of liquid by irradiating IR radiation (30) into the volume of liquid. Thereby it is possible to avoid a depletion zone at the surface and to more accurately measure interactions of the particles with the surface by means of surface-based measurement methods.


Patent
Nano Temper Technologies GmbH and Hirschmann Laborgerate GmbH & Co. KG | Date: 2014-09-12

The invention relates to arrays with a plurality of capillaries being arranged in a plane and mechanically attached to the array, wherein the distance of adjacent capillaries is approximately 2.25 mm or an integer multiple thereof. At least one free end of each capillary projects from the array in such a way that the free ends of the capillaries may be simultaneously inserted into wells of a microwell plate.


Patent
Nano Temper Technologies GmbH | Date: 2013-03-27

The present invention relates to a device for investigating, measuring and/or detecting properties of chemical and/or biological fluids as well as to a device for performing these procedures and a system comprising a holder for holding at least one of said devices. A device for detecting properties of chemical and/or biological fluids may comprise a tubular structure with a cavity for accommodating the fluid, the tubular structure comprising a magnetizable and/or magnetic material, wherein the tubular structure comprises a segment that is transparent to light of wavelength 200nm to 2000nm.


Patent
Hirschmann Laborgerate GmbH & Co. KG and Nano Temper Technologies GmbH | Date: 2012-09-21

The present invention relates to a device for investigating, measuring and/or detecting properties of chemical and/or biological fluids as well as to a device for performing these procedures and a system comprising a holder for holding at least one of said devices. A device for detecting properties of chemical and/or biological fluids may comprise a tubular structure with a cavity for accommodating the fluid, the tubular structure comprising a magnetizable and/or magnetic material, wherein the tubular structure comprises a segment that is transparent to light of wavelength 200 nm to 2000 nm.


Patent
Nano Temper Technologies GmbH | Date: 2015-11-11

The present invention relates generally to a system and a method for thermo-optical measurements in a droplet (23) of aqueous solution comprising particles of interest, the method comprising the following steps: providing the droplet of aqueous solution with a volume of less than 200nl, wherein the aqueous solution is a first liquid (21) and at least a part of the particles of interest are fluorescent particles; embedding the droplet (23) of aqueous solution at least partly in a second liquid (21); irradiating a laser light beam into the droplet to obtain a spatial temperature distribution in the droplet around the irradiated laser light beam; exciting fluorescently said fluorescent particles and detecting the fluorescence at least at one position or at around one position in the droplet or detecting the fluorescence distribution of said fluorescently excited particles, wherein said detection of fluorescence is performed at least once at a predetermined time after the start of the laser irradiation; and determining a characteristic of the particles of interest from the detected fluorescence intensity or fluorescence intensity distribution.


Patent
NanoTemper Technologies GmbH | Date: 2014-11-05

The present invention relates generally to a system and a method for thermo-optical measurements in a droplet of aqueous solution comprising particles of interest, the method comprising the following steps: providing the droplet of aqueous solution with a volume of less than 200 nl, wherein the aqueous solution is a first liquid and at least a part of the particles of interest are fluorescent particles; embedding the droplet of aqueous solution at least partly in a second liquid; irradiating a laser light beam into the droplet to obtain a spatial temperature distribution in the droplet around the irradiated laser light beam; exciting fluorescently said fluorescent particles and detecting fluorescence at least at one position or at around one position in the droplet or detecting the fluorescence distribution of said fluorescently excited particles, wherein said detection of fluorescence is performed at least once at a predetermined time after the start of the laser irradiation; and determining a characteristic of the particles of interest from the detected fluorescence intensity or fluorescence intensity distribution.

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