Montpellier, France
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Chaix A.,National Graduate School of Chemistry, Montpellier | El Cheikh K.,French National Center for Scientific Research | Bouffard E.,French National Center for Scientific Research | Maynadier M.,NanoMedSyn | And 11 more authors.
Journal of Materials Chemistry B | Year: 2016

A novel non-toxic porous silicon nanoparticle grafted with a mannose-6-phosphate analogue and applicable in 2-photon imaging and photodynamic therapy was specifically designed for targeting prostate cancer cells. © 2016 The Royal Society of Chemistry.


Knezevic N.Z.,National Graduate School of Chemistry, Montpellier | Stojanovic V.,Max Mousseron Institute of Biomolecules | Chaix A.,National Graduate School of Chemistry, Montpellier | Bouffard E.,Max Mousseron Institute of Biomolecules | And 8 more authors.
Journal of Materials Chemistry B | Year: 2016

Multifunctionalized porous silicon nanoparticles (pSiNPs), containing the novel Ru(ii) complex-photosensitizer, the polyethylene glycol moiety, and mannose molecules as cancer targeting ligands, are constructed and showcased for application in near infrared (NIR) light-responsive photodynamic therapy (PDT) and imaging of cancer. Exposure to NIR light leads to two-photon excitation of the Ru(ii)-complex which allows efficient simultaneous cancer-imaging and targeted PDT therapy with the functionalized biodegradable pSiNP nanocarriers. © The Royal Society of Chemistry 2016.


Nyalosaso J.L.,Charles Gerhardt Institute | Rascol E.,Charles Gerhardt Institute | Pisani C.,Charles Gerhardt Institute | Pisani C.,CEA Marcoule Nuclear Site | And 13 more authors.
RSC Advances | Year: 2016

Mesoporous Silica Nanoparticles (MSN) are now considered as multifunctional platforms for pharmaceutical development. The goal of this study was to optimize a synthesis procedure to obtain reproducible monodisperse magnetic core@shell Fe3O4@MSN with different coatings and study their uptake by cells. 100 nm core@shell nanoparticles with a unique 18 nm magnetic core were synthesized and covered with PEG groups or coated with a lipid bilayer in a controlled manner and their cellular fate was investigated. Both PEG and lipidic coated nanoparticles exhibit a low toxicity when incubated with Hep-G2 cells compared to pristine ones. Furthermore, the different real-time impedance cellular profiles that were observed and the particles uptake by the cells investigated by TEM suggest different internalization mechanisms or uptake kinetics depending on MSN coverage. This study is a first essential step to ensuring the preparation of well-defined nanomaterials for medical applications; it is considered as a crucial step to be able to perform detailed research about cellular trafficking and signaling pathways. This journal is © The Royal Society of Chemistry 2016.


Croissant J.G.,Charles Gerhardt Institute | Qi C.,Charles Gerhardt Institute | Mongin O.,CNRS Chemistry Institute of Rennes | Hugues V.,CNRS Institute of Molecular Sciences | And 9 more authors.
Journal of Materials Chemistry B | Year: 2015

We report a two-photon-actuated cancer cell killing system that kills the cancer cells via drug delivery through multifunctional mesoporous silica nanogates. Two-photon-sensitive mesoporous organosilica (M2PS) nanocarriers were synthesized via the co-condensation of a silica precursor and a two-photon electron donor. The nanogates were constructed using a fast one-pot process at room temperature on the drug-loaded M2PS nanoparticles (NPs) with the bis(3-triethoxysilylpropyl)disulfide precursor. One and two-photon-actuated cargo releases from the M2PS nanogates were successfully monitored in aqueous solutions. Furthermore, the cellular uptake in MCF-7 cells was demonstrated via two-photon fluorescence imaging of the NPs, which were then applied successfully for drug delivery in cells. © The Royal Society of Chemistry 2015.


Croissant J.G.,Charles Gerhardt Institute | Mauriello-Jimenez C.,Charles Gerhardt Institute | Maynadier M.,NanoMedSyn | Cattoe n X.,CNRS Neel Institute | And 8 more authors.
Chemical Communications | Year: 2015

Biodegradable bridged silsesquioxane (BS) nanomaterials for two-photon-excited (TPE) imaging and therapy of breast cancer cells were described. A versatile synthesis was developed to design monodisperse tetra-alkoxysilylated diamino-diphenylbutadiene or Zn-porphyrin-based nanospheres of 30 to 50 nm. © The Royal Society of Chemistry 2015.


Jimenez C.M.,Charles Gerhardt Institute | Knezevic N.Z.,Charles Gerhardt Institute | Knezevic N.Z.,University of Belgrade | Rubio Y.G.,Charles Gerhardt Institute | And 13 more authors.
Journal of Materials Chemistry B | Year: 2016

In this article, we highlight the properties of nanodiamonds (ND), which were encapsulated in periodic mesoporous organosilica nanoparticles (PMO) and were able to generate reactive oxygen species for photodynamic applications upon two-photon excitation (TPE). The ND@PMO nanoparticles were characterized by various techniques and were then loaded with the anti-cancer drug doxorubicin. The release of the drug was pH sensitive and a synergistic cancer cell killing effect was observed when cancer cells were incubated with doxorubicin-loaded ND@PMO and irradiated with two-photon excitation at 800 nm. © 2016 The Royal Society of Chemistry.


Bouffard E.,Montpellier University | El-Cheikh K.,Montpellier University | Gallud A.,Montpellier University | Da Silva A.,NanoMedSyn | And 5 more authors.
Current Medicinal Chemistry | Year: 2015

Nowadays, nanomedicine brings new opportunities for diagnosis and treatment through innovative combinations of materials structured at the nanoscale, biomolecules and physicochemical processes. If the intrinsic properties of nanomaterials appear of major importance in this new discipline, the functionalization of these nanotools with biomolecules improves both their biocompatibility and efficacy. This is the case of carbohydrate derivatives, natural or synthetic, which are increasingly being used in nanostructures for medical purposes. As in current medicine, sugars are used to mimic their physiological roles. Indeed, carbohydrates enhance the solubility and reduce the clearance of drugs. They are used to mask immunogenic components of nano-objects and escape the body defenses and finally facilitate the delivery to the target tissue. All these properties explain the growing importance of sugars in nanomedicine. © 2015 Bentham Science Publishers.


Perrier M.,Charles Gerhardt Institute | Gallud A.,Montpellier University | Ayadi A.,Montpellier University | Kennouche S.,Montpellier University | And 11 more authors.
Nanoscale | Year: 2015

Cyano-bridged Gd3+/[Fe(CN)6]3- coordination polymer nanoparticles of 3-4 nm stabilized with d-mannitol presenting a high r1 relaxivity value of 11.4 mM-1 s-1 were investigated in vivo as contrast agents (CA) for Magnetic Resonance Imaging (MRI). They allow an increase of the MR image contrast and can act as an efficient intravascular T1 CA with a relatively long blood-circulation lifetime (60 min) without specific toxicity. This journal is © The Royal Society of Chemistry.


Noureddine A.,Charles Gerhardt Institute | Lichon L.,Max Mousseron Institute of Biomolecules | Maynadier M.,NanoMedSyn | Garcia M.,Max Mousseron Institute of Biomolecules | And 4 more authors.
Nanoscale | Year: 2015

The synthesis of mesoporous silica nanoparticles bearing organic functionalities is strained by the careful adjustment of the reaction parameters, as the incorporation of functional and/or voluminous organosilanes during the sol-gel synthesis strongly affects the final structure of the nanoparticles. In this paper we describe the design of new clickable mesoporous silica nanoparticles as spheres or rods, synthesized by the co-condensation of TEOS with two clickable organosilanes (bearing alkyne and azide groups) and readily multi-functionalizable by CuAAC click chemistry. We show that controlled loadings of clickable functions can be homogeneously distributed within the MSN, allowing us to efficiently click-graft various pairs of functionalities while preserving the texture and morphology of the particles. The homogeneous distribution of the grafted functionalities was probed by FRET experiments between two anchored fluorophores. Moreover, a communication by proton transfer between two functions was demonstrated by constructing a light-actuated nanomachine that works through a proton transfer between a photoacid generator and a pH-sensitive supramolecular nanogate. The activation of the nanomachine enabled the successful release of rhodamine B in buffered solutions and the delivery of doxorubicin in breast cancer cells (MCF-7) upon blue irradiation. This journal is © The Royal Society of Chemistry.


PubMed | Charles Gerhardt Institute, CNRS Institute of Molecular Sciences, Montpellier University, CNRS Neel Institute and 2 more.
Type: | Journal: Frontiers in molecular biosciences | Year: 2016

Three dimensional sub-micron resolution has made two-photon nanomedicine a very promising medical tool for cancer treatment since current techniques cause significant side effects for lack of spatial selectivity. Two-photon-excited (TPE) photodynamic therapy (PDT) has been achieved via mesoporous nanoscaffolds, but the efficiency of the treatment could still be improved. Herein, we demonstrate the enhancement of the treatment efficiency via gold-mesoporous organosilica nanocomposites for TPE-PDT in cancer cells when compared to mesoporous organosilica particles. We performed the first comparative study of the influence of the shape and spatial position of gold nanoparticles (AuNPs) with mesoporous silica nanoparticles (MSN) functionalized with thiol groups and doped with a two-photon electron donor (2PS). The resulting multifunctional nanocarriers displayed TPE-fluorescence and were imaged inside cells. Furthermore, mesoporous organosilica NPs decorated gold nanospheres (AuNSs) induced 63 percent of selective killing on MCF-7 breast cancer cells. This study thus provides insights for the design of more effective multifunctional two-photon-sensitive nanocomposites via AuNPs for biomedical applications.

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