Nanomedicine Research Center

Taipei, Taiwan

Nanomedicine Research Center

Taipei, Taiwan
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Khurana B.,Nanomedicine Research Center | Goyal A.K.,Nanomedicine Research Center | Budhiraja A.,Nanomedicine Research Center | Arora D.,Nanomedicine Research Center | Vyas S.P.,Dr Hari Singh Gour University
Current Gene Therapy | Year: 2010

Small interfering RNAs (siRNA) are one of the most recent additions used to silence gene expression. At present, siRNA is the most extensively used gene-silencing technique over other nucleic-acid based approaches to treat diseases including cancer, hepatitis, respiratory disease, cardiovascular diseases, neuronal disease and autoimmune disease. However, systemic delivery of siRNA remains to be the biggest challenge to be overcome. Various strategies have been developed to deliver siRNA efficiently into target cell such as chemical modification of siRNA, physical strategies, viral and non viral-vectors mediated delivery. Among all the approaches non viral vectors including lipoplexes, polyplexes and inorganic nanoparticles were found to be most successful which have been reviewed in this article. Further therapeutic applications of RNAi have also been briefly reviewed. © 2010 Bentham Science Publishers Ltd.

Khan W.,Hebrew University of Jerusalem | Hosseinkhani H.,National Taiwan University of Science and Technology | Ickowicz D.,Hebrew University of Jerusalem | Hong P.-D.,National Taiwan University of Science and Technology | And 2 more authors.
Acta Biomaterialia | Year: 2012

Gene delivery is a promising technique that involves in vitro or in vivo introduction of exogenous genes into cells for experimental and therapeutic purposes. Successful gene delivery depends on the development of effective and safe delivery vectors. Two main delivery systems, viral and non-viral gene carriers, are currently deployed for gene therapy. While most current gene therapy clinical trials are based on viral approaches, non-viral gene medicines have also emerged as potentially safe and effective for the treatment of a wide variety of genetic and acquired diseases. Non-viral technologies consist of plasmid-based expression systems containing a gene associated with the synthetic gene delivery vector. Polysaccharides compile a large family of heterogenic sequences of monomers with various applications and several advantages as gene delivery agents. This chapter, compiles the recent progress in polysaccharide based gene delivery, it also provides an overview and recent developments of polysaccharide employed for in vitro and in vivo delivery of therapeutically important nucleotides, e.g. plasmid DNA and small interfering RNA. © 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Hosseinkhani H.,National Taiwan University of Science and Technology | Hong P.-D.,National Taiwan University of Science and Technology | Yu D.-S.,Nanomedicine Research Center | Chen Y.-R.,National Yang Ming University | And 3 more authors.
International Journal of Nanomedicine | Year: 2012

This study aims to develop a three-dimensional in vitro culture system to genetically engineer mesenchymal stem cells (MSC) to express bone morphogenic protein-2. We employed nanofabrication technologies borrowed from the spinning industry, such as electrospinning, to mass-produce identical building blocks in a variety of shapes and sizes to fabricate electrospun nanofiber sheets comprised of composites of poly (glycolic acid) and collagen. Homogenous nanoparticles of cationic biodegradable natural polymer were formed by simple mixing of an aqueous solution of plasmid DNA encoded bone morphogenic protein-2 with the same volume of cationic polysaccharide, dextran-spermine. Rat bone marrow MSC were cultured on electrospun nanofiber sheets comprised of composites of poly (glycolic acid) and collagen prior to the incorporation of the nanoparticles into the nanofiber sheets. Bone morphogenic protein-2 was significantly detected in MSC cultured on nanofiber sheets incorporated with nanoparticles after 2 days compared with MSC cultured on nanofiber sheets incorporated with naked plasmid DNA. We conclude that the incorporation of nanoparticles into nanofiber sheets is a very promising strategy to genetically engineer MSC and can be used for further applications in regenerative medicine therapy. © 2012 Hosseinkhani et al, publisher and licensee Dove Medical Press Ltd.

Hosseinkhani H.,National Taiwan University of Science and Technology | Chen Y.-R.,National Yang Ming University | He W.,National Taiwan University of Science and Technology | Hong P.-D.,National Taiwan University of Science and Technology | And 2 more authors.
Journal of Nanoparticle Research | Year: 2013

This study aims to engineer novel targeted delivery system composed of magnetic DNA nanoparticles to be effective as an efficient targeted gene therapy vehicle for tumor therapy. A polysaccharide, dextran, was chosen as the vector of plasmid DNA-encoded NK4 that acts as an HGF-antagonist and anti-angiogenic regulator for inhibitions of tumor growth, invasion, and metastasis. Spermine (Sm) was chemically introduced to the hydroxyl groups of dextran to obtain dextran-Sm. When Fe2+ solution was added to the mixture of dextran-Sm and a plasmid DNA, homogenous DNA nanoparticles were formed via chemical metal coordination bonding with average size of 230 nm. Characterization of DNA nanoparticles was performed via dynamic light scattering measurement, electrophoretic light scattering measurement, as well as transmission electron microscope. DNA nanoparticles effectively condensed plasmid DNA into nanoparticles and enhanced the stability of DNA, while significantly improved transfection efficiency in vitro and tumor accumulation in vivo. In addition, magnetic DNA nanoparticles exhibited high efficiency in antitumor therapy with regards to tumor growth as well as survival of animals evaluated in the presence of external magnetic field. We conclude that the magnetic properties of these DNA nanoparticles would enhance the tracking of non-viral gene delivery systems when administrated in vivo in a test model. These findings suggest that DNA nanoparticles effectively deliver DNA to tumor and thereby inhibiting tumor growth. © 2013 Springer Science+Business Media Dordrecht.

Hosseinkhani H.,National Taiwan University of Science and Technology | Hiraoka Y.,Nitta Gelatin Inc. | Li C.-H.,Graduate Institute of Dental Medicine | Chen Y.-R.,Tzu Chi Hospital | And 3 more authors.
ACS Chemical Neuroscience | Year: 2013

Engineering the cellular microenvironment has great potential to create a platform technology toward engineering of tissue and organs. This study aims to engineer a neural microenvironment through fabrication of three-dimensional (3D) engineered collagen matrixes mimicking in-vivo-like conditions. Collagen was chemically modified with a pentapeptide epitope consisting of isoleucine-lysine-valine-alanine-valine (IKVAV) to mimic laminin structure supports of the neural extracellular matrix (ECM). Three-dimensional collagen matrixes with and without IKVAV peptide modification were fabricated by freeze-drying technology and chemical cross-linking with glutaraldehyde. Structural information of 3D collagen matrixes indicated interconnected pores structure with an average pore size of 180 μm. Our results indicated that culture of dorsal root ganglion (DRG) cells in 3D collagen matrix was greatly influenced by 3D culture method and significantly enhanced with engineered collagen matrix conjugated with IKVAV peptide. It may be concluded that an appropriate 3D culture of neurons enables DRG to positively improve the cellular fate toward further acceleration in tissue regeneration. © 2013 American Chemical Society.

Sharma S.,Nanomedicine Research Center | Kumar P.,Nanomedicine Research Center | Jaiswal A.,CSIR - Central Electrochemical Research Institute | Dube A.,CSIR - Central Electrochemical Research Institute | Gupta S.,Nanomedicine Research Center
Journal of Biomedical Nanotechnology | Year: 2011

Visceral leishmaniasis (VL) is the most severe of all the forms of leishmaniasis and usually lethal if untreated. The present work aimed to develop and characterize doxorubicin loaded mannan conjugated microparticles against experimental visceral leishmaniasis for selective and targeted delivery of doxorubicin to the macrophages of liver and spleen for the effective chemotherapy of VL. Macrophage targeting using doxorubicin loaded PLGA-microparticles would certainly improve the chemotherapy with reduced side effects against VL. Copyright © 2011 American Scientific Publishers All rights reserved.

Hosseinkhani H.,National Taiwan University of Science and Technology | Hong P.-D.,National Taiwan University of Science and Technology | Yu D.-S.,Nanomedicine Research Center
Chemical Reviews | Year: 2013

A chemical review considers the self-assembled systems and the latest developments for their potential applications in regenerative medicine. Self-assembly is a native process, which can be classified into two types, such as static and dynamic. Static selfassembly contributes to systems that are at global or local equilibrium and do not dissipate energy. Dynamic self-assembly occurs when formation of an ordered state of equilibrium requires dissipation of energy. Interactions responsible for formation of structures or patterns between components occur only if the system dissipates energy lead to dynamic self-assembly. Self-assembly also takes place at molecular, mesoscopic, and macroscopic scales. Self-assembly has been classified as molecular and nanoscale self-assemblies and meso- and macroscopic self-assemblies on the basis of this criteria.

Sharma A.,National Physical Laboratory India | Sharma A.,Nanomedicine Research Center | Gupta A.,National Physical Laboratory India | Rath G.,Nanomedicine Research Center | And 3 more authors.
Journal of Materials Chemistry B | Year: 2013

The intention of the present investigation was to develop an oral formulation for an anti-diabetic drug that not only could deliver it in the active form but also provide a sustained and controlled release profile. A biodegradable poly(vinyl alcohol) (PVA) and sodium alginate (NaAlg) electrospun composite nanofiber based transmucosal patch was developed and the anti-diabetic drug insulin was loaded in it by active loading. The drug entrapment in the composite nanofibers during the processing was confirmed by scanning electron microscopy, atomic force microscopy, X-ray diffraction and Fourier transform infrared spectroscopy. The in vivo studies were carried on male Wistar rats by the sublingual route. The mucoadhesive strength results confirmed that the drug loaded PVA-NaAlg nanofiber patch had the highest strength among the PVA, PVA-NaAlg and drug loaded PVA-NaAlg samples, due to its higher water holding capacity. The in vitro activity provided a sustained and controlled release pattern of the drug from the nanofiber patch. In vivo activity validated the fact that insulin was delivered in its active state and showed appreciable results when compared to the commercial formulation. The insulin release follows first order kinetics followed by an initial burst release necessary to produce the desired therapeutic activity. Furthermore an encapsulation efficacy of 99% of the experimental formulation provides sufficient indication that the composite nanofibers serve as an ideal carrier for the delivery of insulin via the sublingual route. Thus the present investigation gives impetus to work in the direction of delivering anti-diabetic drugs (proteins and peptides) via the oral route using electrospun composite nanofiber transmucosal patches. © 2013 The Royal Society of Chemistry.

Aggarwal S.,Nanomedicine Research Center | Yadav S.,Nanomedicine Research Center | Gupta S.,Nanomedicine Research Center
Journal of Biomedical Nanotechnology | Year: 2011

The present study aimed to prepare and characterize anti EGFR monoclonal antibody (mab) conjugated Gemcitabine loaded PLGA nanoparticles for their selective delivery to pancreatic cells and evaluation of the systems in vitro. It was observed that direct covalent coupling of antibodies to glutaraldehyde activated nanoparticles is an appropriate method to achieve cell-type specific drug carrier systems based on polymeric nanoparticles that have potential to be applied for targeted chemotherapy in EGFR positive cancer. Copyright © 2011 American Scientific Publishers All rights reserved.

Bhardwaj A.,Nanomedicine Research Center
Artificial cells, nanomedicine, and biotechnology (Print) | Year: 2013

Tuberculosis (TB) remains one of the oldest and deadliest diseases in the current scenario. The intracellular organism Mycobacterium tuberculosis, which mainly resides in mononuclear phagocytes, is responsible for tuberculosis in humans. A few therapies are available for the treatment of tuberculosis but they have many hurdles. To overcome these hurdles, a combination of chemotherapeutic agent-loaded vesicular systems have been prepared to overcome tuberculosis. To investigate the role of novel drug delivery systems for the treatment of pulmonary tuberculosis, ligand appended liposomals have been developed. In the present study, drug-loaded, ligand-appended liposomes and their DPI (Dry Powder Inhaler) forms have been prepared and characterized using various in vitro and in vivo parameters. The prepared ligand-appended liposomal formulation showed good entrapment efficiency, prolonged drug release, improved recovery of drugs from the target site, and proved to be more suitable for use as DPI, justifying their potential for improved drug delivery. Thus we tried our best by our project to reduce the national burden of tuberculosis, which is still a global health challenge.

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