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Collins J.M.,NanoInk | Nettikadan S.,NanoInk
Analytical Biochemistry | Year: 2011

Tip-based direct protein printing is a relatively new technique that is useful for controlling the cellular microenvironment with subcellular resolution. Coculture studies have been useful for mimicking the in vivo environment and studying effects on stem or progenitor cell function. However, there are many experimental variables that cannot be properly controlled and may lead to confounding results. Here we demonstrate a technique that allows spatial control of multiple cell types at single cell levels on a substrate. Specifically, 3T3 fibroblasts and C2C12 myoblasts and their respective binding dynamics with fibronectin and laminin demonstrate the single cell coculture concept. © 2011 Elsevier Inc. All rights reserved. Source

Hung S.-C.,NanoInk | Nafday O.A.,NanoInk | Haaheim J.R.,NanoInk | Ren F.,University of Florida | And 2 more authors.
Journal of Physical Chemistry C | Year: 2010

We report the first demonstration of subμm, sub-50-μΩ·cm conductive traces directly written by Dip Pen Nanolithography (DPN). We achieved subμm Ag lines with 28.8 μΩ·cm average resistivity after direct-write printing from a silver nanoparticle-based ink suspension and annealing at 150°C for 10 min. This compares to Ag bulk resistivity of 1.63 μΩ·cm, where the difference is within the range of previously reported variations in conductivity of Ag-based inks due to annealing conditions and larger width scales. We leveraged DPN's ability to directly place materials at specific locations in order to fabricate and characterize these conductive silver (Ag) traces on electrode patterns and multiple substrates (SiO 2, Kapton, mica). The low viscosity of the AgNP ink solution allowed write speeds up to 1600 μm/s, almost 4 orders of magnitude higher than typical thiol-on-gold DPN writing speeds. This direct-write methodology paves the way for site-specific deposition of metallic materials for use in applications such as circuit repair, sensor element functionalization, failure analysis, gas sensing, and printable electronics. © 2010 American Chemical Society. Source

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