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San Diego, CA, United States

Samberg M.E.,North Carolina State University | Samberg M.E.,University of North Carolina at Chapel Hill | Oldenburg S.J.,Nanocomposix, Inc. | Monteiro-Riviere N.A.,North Carolina State University | Monteiro-Riviere N.A.,University of North Carolina at Chapel Hill
Environmental Health Perspectives | Year: 2010

INTRODUCTION: Products using the antimicrobial properties of silver nanoparticles (Ag-nps) may be found in health and consumer products that routinely contact skin. OBJECTIVES: This study was designed to assess the potential cytotoxicity of Ag-nps in human epidermal keratinocytes (HEKs) and their inflammatory and penetrating potential into porcine skin in vivo. MATERIALS AND METHODS: We used eight different Ag-nps in this study [unwashed/uncoated (20, 50, and 80 nm particle diameter), washed/uncoated (20, 50, and 80 nm), and carbon-coated (25 and 35 nm)]. Skin was dosed topically for 14 consecutive days. HEK viability was assessed by MTT, alamarBlue (aB), and CellTiter 96 AQueous One (96AQ). Release of the pro inflammatory mediators interleukin (IL)-1β, IL-6, IL-8, IL-10, and tumor necrosis factor-α (TNF-α) were measured. RESULTS: The effect of the unwashed Ag-nps on HEK viability after a 24-hr exposure indicated a significant dose-dependent decrease (p < 0.05) at 0.34 μg/mL with aB and 96AQ and at 1.7 μg/mL with MTT. However, both the washed Ag-nps and carbon-coated Ag-nps showed no significant decrease in viability at any concentration assessed by any of the three assays. For each of the unwashed Ag-nps, we noted a significant increase (p < 0.05) in IL-1β, IL-6, IL-8, and TNF-α concentrations. We observed localization of all Ag-nps in cyto plasmic vacuoles of HEKs. Macroscopic observations showed no gross irritation in porcine skin, whereas microscopic and ultrastructural observations showed areas of focal inflammation and localization of Ag-nps on the surface and in the upper stratum corneum layers of the skin. CONCLUSION: This study provides a better understanding Ag-nps safety in vitro as well as in vivo and a basis for occupational and risk assessment. Ag-nps are non toxic when dosed in washed Ag-nps solutions or carbon coated. Source


Grant
Agency: Department of Defense | Branch: Defense Health Program | Program: SBIR | Phase: Phase I | Award Amount: 150.00K | Year: 2015

Lyme disease is the most common tick-borne disease in North America and one of the fast-growing infectious diseases in the United States. An inexpensive, portable and accurate diagnostic device for the detection of Borrelia bacteria in the deer tick will be developed. The diagnostic device will consist of an efficient extractor for disrupting the hard exoskeleton on the tick, an ultra-sensitive lateral flow based detection assay and a cell phone reader attachment for quantifying and geotagging the test strip results. The detection kit will have a small form factor, will be simple to use and will require minimal power.


Patent
Nanocomposix, Inc. | Date: 2012-07-19

A pyrophoric sheet that comprises oxidizable iron, non-combustible fibers, stiction-reducing coating where the sheet has a water content<2%.


Patent
Nanocomposix, Inc. | Date: 2015-12-18

Embodiments of the present invention relate to methods for preparing high optical density solutions of nanoplates, such as silver nanoplates or silver platelet nanoparticles, and to nanoparticles, solutions and substrates prepared by said methods. The process can include the addition of stabilizing agents (e.g., chemical or biological agents bound or otherwise linked to the nanoparticle surface) that stabilize the nanoparticle before, during, and/or after concentration, thereby allowing for the production of a stable, high optical density solution of silver nanoplates. The process can also include increasing the concentration of silver nanoplates within the solution, and thus increasing the solution optical density.


Patent
Nanocomposix, Inc. and Sienna Labs Inc. | Date: 2015-04-08

Embodiments of the present invention relate to methods for preparing high optical density solutions of nanoparticle, such as nanoplates, silver nanoplates or silver platelet nanoparticles, and to the solutions and substrates prepared by the methods. The process can include the addition of stabilizing agents (e.g., chemical or biological agents bound or otherwise linked to the nanoparticle surface) that stabilize the nanoparticle before, during, and/or after concentration, thereby allowing for the production of a stable, high optical density solution of silver nanoplates. The process can also include increasing the concentration of silver nanoplates within the solution, and thus increasing the solution optical density.

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