Wu X.,Nanjing University of Science and Technology |
Shu H.,Nanjing University of Science and Technology |
Zhou B.,NanjingUniversity of Science & Technology |
Geng Y.,NanjingUniversity of Science & Technology |
And 2 more authors.
Sensors and Actuators, B: Chemical
A new rhodamine B-based fluorescent probe (Probe-M), namely N-(2-(3′,6′-bis(diethylamino)-3-oxospiro[isoindoline-1,9′-xanthen]-2-yl)ethyl)-3,5-bis(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl) benzamide, was designed and synthesized to develop a fluorescent sensor for glutathione (GSH). Probe-M displayed excellent selectivity and sensitivity for GSH over many other amino acids in an EtOH/HEPES buffer solution. The application of GSH in cellular imaging experiments indicated that the probe has appreciable cell permeability and is highly responsive to changes in the GSH level. As a result, Probe M can serve as a fluorescent probe for detecting GSH in living cells. © 2016 Elsevier B.V. Source