Nanjing Stomatological Hospital

Nanjing, China

Nanjing Stomatological Hospital

Nanjing, China
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Bai L.-M.,China Pharmaceutical University | Huang X.-F.,Nanjing Stomatological Hospital | Xu H.-M.,China Pharmaceutical University
Chinese Pharmacological Bulletin | Year: 2014

Aim: To investigate the expression of CD31, CD105, HIF-1α and VEGF in xenografts tumor of HT-29 tumor cells during different stages of growth. Methods: HT-29 tumor cells were transplanted into nude mice, the tumor was removed when the tumor volumes reached <100 mm3, 100-300 mm 3 and >300 mm3 respectively. Immunochemical method was adopted to detect the expression of CD31, CD105, HIF-1α and VEGF. Results: HT-29 xenografts tumor volumes <100 mm3, 100-300 mm3, and >300 mm3 showed expressions with CD31-MVD at 37.40±4.17, 18.80±1.72 and 14.20±2.23 respectively; CD105-MVD at 22.80±3.54, 15.60±1.35 and 10.20±2.48; positive expression rate of VEGF was 26.20%±0.83%, 40.73%±6.29% and 13.41%±1.20% respectively; while positive expression rate of HIF-1α was 3.20%±2.97%, 11.89%±1.94% and 80.62%±3.47% respectively. On the other hand, for different volumes group, CD31-MVD, CD105-MVD, VEGF and HIF-1α expression ratios had significant differences (P<0.01). Conclusions: The expression of MVD and vascular-related factors within the tumor caused by HT-29 xenografts tumor in nude mice at different growth stages was varied. There was a certain correlation between tumor volume and MVD, VEGF, HIF-1α.

Zou D.,Tongji University | Zou D.,Shanghai JiaoTong University | Zou D.,Anhui Medical University | Zhang Z.,Shanghai JiaoTong University | And 14 more authors.
Biomaterials | Year: 2011

Tissue engineering combined with gene therapy represents a promising approach for bone regeneration. The Hypoxia-inducible factor-1α (HIF-1α) gene is a pivotal regulator of vascular reactivity and angiogenesis. Our recent study has showed that HIF-1α could promote osteogenesis of bone mesenchymal stem cells (BMSCs) using a gene point mutant technique. To optimize the function of HIF-1α on inducing stem cells, another constitutively active form of HIF-1α (CA5) was constructed with truncation mutant method and its therapeutic potential on critical-sized bone defects was evaluated with calcium-magnesium phosphate cement (CMPC) scaffold in a rat model. BMSCs were treated with Lenti (lentivirus) -CA5, Lenti-WT (wild-type HIF-1α), and Lenti-LacZ. These genetically modified BMSCs were then combined with CMPC scaffolds to repair critical-sized calvarial defects in rats. The results showed that the overexpression of HIF-1α obviously enhanced the mRNA and protein expression of osteogenic markers in vitro and robust new bone formation with the higher local bone mineral density (BMD) was found in vivo in the CA5 and WT groups. Furthermore, CA5 showed significantly greater stability and osteogenic activity in BMSCs compared with WT. These data suggest that BMSCs transduced with truncation mutanted HIF-1α gene can promote the overexpression of osteogenic markers. CMPC could serve as a potential substrate for HIF-1α gene modified tissue engineered bone to repair critical sized bony defects. © 2011 Elsevier Ltd.

Zou D.,Tongji University | Han W.,Nanjing Stomatological Hospital | You S.,Tongji University | Ye D.,Shanghai JiaoTong University | And 7 more authors.
Cell Proliferation | Year: 2011

Objectives: Hypoxia-inducible factor 1α (HIF-1α) is a pivotal regulator of hypoxic and ischaemic vascular responses that drives transcriptional activation of hundreds of genes involved in vascular reactivity, angiogenesis and arteriogenesis. Previous reports based on gene knockout technology have demonstrated that HIF-1α can promote osteogenesis. However, this protein is easily degraded in a normoxic state, which makes in vitro studies of HIF-1α-induced mesenchymal stem cell (MSC) osteogenesis difficult. For better understanding of HIF-1α promoting osteogenesis, the role of HIF-1α-induced MSC osteogenesis in the normoxic state has been investigated here. Materials and methods: HIF-1α was made to overexpress using a lentiviral vector, and its effects on bone marrow-derived mesenchymal stem cell (BMSC) osteogenesis were investigated. Real-time quantitative and western blotting (to assess expression levels of angiogenic and osteogenic related genes regulated by Lenti-HIF-1α), alkaline phosphatase (ALP) and alizarin red-S staining analyses, were performed. Results: In HIF-1α gene-transfected BMSCs, expression levels of angiogenic, cartilaginous and osteogenic genes were all increased significantly compared to Lenti LacZ-transfected cells, at both mRNA and protein levels. ALP activity and alizarin red-S staining were significantly enhanced in HIF-1α transduced cells compared to control cells, on day 21. Conclusions: These results indicate that Lenti-HIF-1α can induce BMSC overexpression levels of angiogenic and osteogenic genes in vitro in the normoxic state. Further study will be focused on whether HIF-1α can also improve bone repair in vivo. © 2011 Blackwell Publishing Ltd.

Zou D.,Tongji University | Zou D.,Shanghai JiaoTong University | Zou D.,Anhui Medical University | Zhang Z.,Shanghai JiaoTong University | And 14 more authors.
Stem Cells | Year: 2011

The processes of angiogenesis and bone formation are coupled both temporally and spatially during bone repair. Bone marrow-derived mesenchymal stem cells (BMSCs) have been effectively used to heal critical-size bone defects. Enhancing their ability to undergo angiogenic and osteogenic differentiation will enhance their potential use in bone regeneration. Hypoxia-inducible factor-1α (HIF-1α) has recently been identified as a major regulator of angiogenic-osteogenic coupling. In this study, we tested the hypothesis that HIF-1α gene therapy could be used to promote the repair of critical-sized bone defects. Using lentivirus-mediated delivery of wild-type (HIF) or constitutively active HIF-1α (cHIF), we found that in cultured BMSCs in vitro, HIF and cHIF significantly enhanced osteogenic and angiogenic mRNA and protein expression when compared with the LacZ group. We found that HIF-1α-overexpressing BMSCs dramatically improved the repair of critical-sized calvarial defects, including increased bone volume, bone mineral density, blood vessel number, and blood vessel area in vivo. These data confirm the essential role of HIF-1α modified BMSCs in angiogenesis and osteogenesis in vitro and in vivo. © AlphaMed Press.

Zou D.,Tongji University | Zou D.,Shanghai JiaoTong University | Zou D.,Anhui Medical University | Zhang Z.,Shanghai JiaoTong University | And 17 more authors.
Biomaterials | Year: 2012

The successful clinical outcome of the implanted tissue-engineered bone is dependent on the establishment of a functional vascular network. A gene-enhanced tissue engineering represents a promising approach for vascularization. Our previous study indicated that hypoxia-inducible factor-1α (HIF-1α) can up-regulate the expression of vascular endothelial growth factor (VEGF) and stromal-derived factor 1 (SDF-1) in bone mesenchymal stem cells (BMSCs). The angiogenesis is a co-ordinated process that requires the participation of multiple angiogenic factors. To further explore the angiogenic effect of HIF-1α mediated stem cells, in this study, we systematically evaluated the function of HIF-1α in enhancing BMSCs angiogenesis invitro and invivo. A constitutively active form of HIF-1α (CA5) was inserted into a lentivirus vector and transduced into BMSCs, and its effect on vascularization and vascular remodeling was further evaluated in a rat critical-sized calvarial defects model with a gelatin sponge (GS) scaffold. The expression of the key angiogenic factors including VEGF, SDF-1, basic fibroblast growth factor (bFGF), placental growth factor (PLGF), angiopoietin 1 (ANGPT1), and stem cell factor (SCF) at both mRNAs and proteins levels in BMSCs were significantly enhanced by HIF-1α overexpression compared to the invitro control group. In addition, HIF-1α-over expressing BMSCs showed dramatically improved blood vessel formation in the tissue-engineered bone as analyzed by photography of specimen, micro-CT, and histology. These data confirm the important role of HIF-1α in angiogenesis in tissue-engineered bone. Improved understanding of the mechanisms of angiogenesis may offer exciting therapeutic opportunities for vascularization, vascular remodeling, and bone defect repair using tissue engineering strategies in the future. © 2011 Elsevier Ltd.

Wang Z.-Y.,Nanjing Stomatological Hospital | Shi P.-H.,Nanjing Stomatological Hospital | Huang X.-F.,Nanjing Stomatological Hospital | Hua Z.-C.,Nanjing University | And 3 more authors.
International Journal of Oral and Maxillofacial Surgery | Year: 2010

Vascular endothelial growth factor (VEGF) may cause functional deficiency in dendritic cells (DCs) in vitro. The roles of peripheral blood dendritic cells (PBDCs) and VEGF in patients with oral squamous cell carcinoma (OSCC) are not well understood. The authors analysed the correlation between VEGF and PBDC in 81 OSCC patients. They assessed the effect of VEGF on DC function in vitro. VEGF levels were significantly increased in OSCC patients compared with control subjects (P < 0.05), but PBDC levels were significantly lower (P < 0.05). VEGF expression in TNM I-II (67%) and T1-T2 (74%) was significantly lower, compared with TNM III-IV (88%, P < 0.05) and T3-T4 (89%, P < 0.05). Increased VEGF expression in primary tumours was significantly correlated with elevated serum VEGF levels, but reduced PBDC levels. In vitro cultured DC exposed to VEGF showed significantly decreased expression of functional proteins, enhanced endocytosis activity, and elicited weaker proliferation of T cells, compared with that of free VEGF (P < 0.01). These findings suggest that decreased PBDC and elevated VEGF occur in OSCC patients. Higher VEGF levels may affect precursor cells, resulting in decreased numbers of functional DC. © 2010 International Association of Oral and Maxillofacial Surgeons.

Yuan T.,Nanjing University of Aeronautics and Astronautics | Liao W.,Nanjing University of Aeronautics and Astronautics | Dai N.,Nanjing University of Aeronautics and Astronautics | Cheng X.,Nanjing University of Aeronautics and Astronautics | Yu Q.,Nanjing Stomatological Hospital
International Journal of Biomedical Imaging | Year: 2010

An integrated single-tooth modeling scheme is proposed for the 3D dental model acquired by optical digitizers. The cores of the modeling scheme are fusion regions extraction, single tooth shape restoration, and single tooth separation. According to the valley shape-like characters of the fusion regions between two adjoining teeth, the regions of the 3D dental model are analyzed and classified based on the minimum curvatures of the surface. The single tooth shape is restored according to the bioinformation along the hole boundary, which is generated after the fusion region being removed. By using the extracted boundary from the blending regions between the teeth and soft tissues as reference, the teeth can be separated from the 3D dental model one by one correctly. Experimental results show that the proposed method can achieve satisfying modeling results with high-degree approximation of the real tooth and meet the requirements of clinical oral medicine. Copyright © 2010 Tianran Yuan et al.

Jiang M.,University of Sichuan | Yang Z.,Nanjing Stomatological Hospital | Feng B.,University of Sichuan | You M.,University of Sichuan | Wang H.,University of Sichuan
Journal of Voice | Year: 2015

Objective Velopharyngeal closure is an important physiological process contributing to the normal function of speech and swallowing. The aim of this study was to analyze the influence of sound intensity on velopharyngeal function in normal individuals. Materials and methods Lateral cephalograms of 38 volunteers obtained at rest and during phonation of vowel /i:/ at both high and low sound intensity were carefully analyzed. The digital sound level meter was used to evaluate and record the sound intensity of the phonation process. The angular and linear parameters on the lateral cephalograms were then measured to reveal the correlation between sound intensity and velopharyngeal closure. Results All the angular parameter values measured in the study were significantly greater in high sound intensity condition. As for linear parameters, all values were found to be significantly larger at high sound intensity, except for the effective velopharyngeal length and the vertical velopharyngeal length. A multiple linear regression model was set up to describe the correlation between the sound intensity, the effective velopharyngeal length, and velopharyngeal closure. With the increase of sound intensity and the decrease of the effective velopharyngeal length, the width of velopharyngeal closure is enlarged. Conclusions As one of the characteristic features of sound wave, the sound intensity was found to affect the objectively measured parameters of velopharyngeal closure on lateral cephalograms. © 2015 The Voice Foundation.

Yuan T.,Nanjing University of Aeronautics and Astronautics | Liao W.,Nanjing University of Aeronautics and Astronautics | Cheng X.,Nanjing University of Aeronautics and Astronautics | Dai N.,Nanjing University of Aeronautics and Astronautics | Yu Q.,Nanjing Stomatological Hospital
Jisuanji Fuzhu Sheji Yu Tuxingxue Xuebao/Journal of Computer-Aided Design and Computer Graphics | Year: 2010

A comprehensive tooth shape modeling scheme is presented to reconstruct the missing part of each tooth due to teeth adhesion on the 3D dental model. The adhesion region between two adjacent teeth on the 3D dental model is first detected by exploiting the minimum principal curvature information. Then, the adhesion region is cut off and the tooth profile opening caused by the removal of the adhesion region is filled with triangular patches adopting a local optimization scheme, it is then refined to form an intermediate restoration surface patch. The final restoration result is obtained by minimizing the surface energy to reflect the bio-characteristics of the single tooth. Experimental results demonstrate that the missing profile shape of a single tooth on the 3D dental model can be restored faithfully, thus meeting the requirements of dentistry, oral surgery & medicine.

PubMed | Nanjing Stomatological Hospital
Type: Journal Article | Journal: Journal of prosthodontics : official journal of the American College of Prosthodontists | Year: 2015

Wear, extraction, or fracture of all or part of a mandibular first molar can lead to the supraeruption of the opposing maxillary molar, resulting in occlusal interference and lack of restoration space. This report describes a method to gain sufficient vertical space for permanent restoration. A direct composite resin restoration was placed on the occlusal surface of a lower molar, intentionally making the interim restoration high and intruding the maxillary molar. After 6 weeks, the extruded tooth returned to the desired position, and functional occlusion was restored, enabling a ceramic restoration on the mandibular molar. No marked adverse sensory reaction was reported in this therapeutic process, and no deleterious signs were detected in the teeth, periodontium, or temporomandibular joints. The simple treatment type was effective, noninvasive, and time saving, while also preserving maximum tooth structures.

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